Effect of antibiotics on polymorphonuclear leukocyte functions and myeloperoxidase activity, glutathione and malondialdehyde levels in allergic asthma

• Autorzy: Rayaman P. , Rayaman E. , Cevikbas A. , Demirtunc R. , Sehirli A.O. , Alagoz S.G. , Gurer U.S.
• Języki publikacji: EN

Abstrakty

EN

We investigated the effect of ciprofloxacin, rifampicine and doxycycline on myeloperoxidase (MPO) activity, glutathione (GSH) and malondialdehyde (MDA) levels in allergic asthma patients and healthy volunteers. Polymorphonuclear leukocytes(PMNs) were isolated with ficoll-hypaque gradient centrifugation method. MPO activity was assayed with modified o-dianisidine, GSH by Ellman’s and MDA levels by Beuge’s method. PMN functions and MDA levels of patients significantly decreased when compared with healthy volunteers. Ciprofloxacin significantly increased PMN functions, MPO activity and MDA levels of both groups. We have demonstrated that ciprofloxacin has beneficial effects on MPO activity and PMN functions in allergic asthma patients and healthy volunteers.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2015
• Tom: 64
• Numer: 1
• Opis fizyczny: p.69-72,ref.

Twórcy

autor

Rayaman P.

• Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Marmara University, Haydarpasa-Istanbul, Turkey

autor

Rayaman E.

• Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Marmara University, Haydarpasa-Istanbul, Turkey

autor

Cevikbas A.

• Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Marmara University, Haydarpasa-Istanbul, Turkey

autor

Demirtunc R.

• Department of Internal Medicine, Haydarpasa Numune Training and Research Hospital, Istanbul, Turkey

autor

Sehirli A.O.

• Department of Pharmacology, Faculty of Pharmacy, Marmara University, Haydarpasa-Istanbul, Turkey

autor

Alagoz S.G.

• Department of Pneumonology, Haydarpasa Numune Training and Research Hospital, Istanbul, Turkey

autor

Gurer U.S.

• Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Marmara University, Haydarpasa-Istanbul, Turkey

Bibliografia

• Alexander J.W., D.B. Windhorst and R.A. Good. 1968. Improved tests for the evaluation of neutrophil function in human disease. J. Lab. Clin. Med. 72(1): 36–48.
• Badur S. 1991. Deleterious effects of antibiotics on immune system (in Turkish ). Klimik Derg. 4(3): 105–108.
• Barbior B.M. and H.J. Cohen. 1981. Measurement of Neutrophil Function: Phagocytosis, Degranulation, the Respiratory Burst and Bacterial Killing. 1st ed., pp. 1–38. In: Cline M.J.(Ed). Methods in Hematology, Leukocyte Function Churchill Livingstone, New York. Beier J., K.M. Beeh and D. Semmler. 2004. Increased concentrations of glutathione in induced sputum of patients with mild or moderate allergic asthma. Ann. Allergy. Asthma. Immunol. 92(4): 459–463.
• Beuge J.A. and S.D. Aust. 1978. Microsomal lipid peroxidation, Meth. Enzymol. 52: 302–311.
• Beutler E. 1975. Glutathione in Red Blood Cell Metabolism. pp.112–114. In: A Manual of Biochemical Methods. Grune&Stratton, New York.
• Boner A.L., D.G. Peroni and G.L. Piacentini. 1993. Influence of allergen avoidance at high altitude on serum markers of eosinophil activation in children with allergic asthma. Clin. Exp. Allergy. 23(12): 1021–1026.
• Daşdelen N., Ü.S. Gürer and A. Çevikbaş. 1999. In vitro investigation of antibiotic combinations that have inhibitory and immunomodulatory effects on human PMN function (in Turkish ). Türk Mikrob. Cem. Derg. 29: 17–22.
• Gemmell C.G. 1993. Antibiotics and neutrophil function-potential immunomodulating activities. J. Antimicrob. Chemother. Feb; 31 Suppl. B: 23–33.
• Gürer Ü.S., S. Büyüköztürk and Ş. Palandüz. 2005. The effects of allergen-specific immunotherapy on polymorphonuclear leukocyte functions in patients with seasonal allergic rhinits. Int. Immunopharmacol. 5: 661–666.
• Gürer Ü.S., P. Göçer and E. Erçağ. 2006. Effects of some antibiotics on polymorphonuclear leukocyte functions of elderly patients in vitro before and after zinc suplementation. Int. Immunopharmacol. 6: 808–816.
• Işık H., A. Cevikbaş and U.S. Gürer. 2010.Potential adjuvant effects of Nigella sativa seeds to improve specific immunotherapy in allergic rhinitis patients. Med. Princ. Pract. 19(3): 206–11.
• Kämpe M., I. Stolt, M. Lampinen and M. Carlson. 2011. Patients with allergic rhinitis and allergic asthma share the same pattern of eosinophil and neutrophil degranulation after allergen challenge. Clin. Mol. Allergy. 9(3):1–10.
• Kurutas E.B., A. Cetinkaya and E. Bulbuloglu. 2005. Effects of antioxidant therapy on leukocyte myeloperoxidase and Cu/Zn-Superoxide dismutase and plasma malondialdehyde levels in experimental colitis. Mediators. Inflamm. 14(6): 390–394.
• Lowry O.H., N.J. Rosebrough and A. Farr. 1951.Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193(1): 265–275.
• Okur E., F. Inanc and M.A. Kilic. 2006. Malondialdehyde level and adenosine deaminase activity in nasal polyps. Otolaryngol. Head. Neck. Surg. 134(1):37–40.
• Öztop A., A. Demir and N. Saydam. 2002 .Relationship between serum gluthatione peroxidase, superoxide dysmutase, malonyldialdehyde levels and severety in allergic asthma (in Turkish). Sol. Hastalıkları 13: 239–245.
• Richardson M.D., G. Scott and G.S. Shankland. 1992. Effect of ciloftragin on phagocytosis and intracellular killing of Candida albicans by human neutrophilis. Eur. J. Clin. Microbiol. Infect. Dis. 11(1): 22–26.
• Shurtz-Swirski R., S. Sela, A.T. Herskovits, S.M. Shasha, G. Shapiro, L. Nasser and B. Kristal. 2001. Involvement of peripheral polymorphonuclear leukocytes in oxidative stress and inflammation in type 2 diabetic patients. Diabetes Care 24(1): 104–110.
• Szczepaniak W., W. Medrala and A. Wolańczyk-Medrala. 2003. Neutrophil myeloperoxidase release by selected extrinsic allergens. Pol. Merkur. Lekarski 14(79): 31–35.