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2002 | 07 | 4 |

Tytuł artykułu

Reactive oxygen species upregulate expression of PAI-1 in endothelial cells

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Second messengers involved in the signal transduction pathway leading to induction of the plasminogen activator inhibitor (PAI-1) have not yet been well characterized. This study focuses on the mechanisms of regulation of PAI-1 expression by reactive oxygen species (ROS) in human endothelial cells. Inhibition of the tumor necrosis factor α (TNFα)-induced expression of PAI-1 by antioxidant N-acetyl-L-cysteine (NAC) indicated redox-sensitive mechanisms involved in the signalling pathway. Because TNFα induces PAI-1 production in endothelial cells, and NAC attenuated this response, we attempted to investigate the possible involvement of ROS in the activation of PAI-1 by TNFα. Upregulation of PAI-1 expression in endothelial cells by the stimulation with TNFα (50ng/ml) or H2O2 (10-200µM), observed by measurement of the antigen and mRNA levels, was reversed in the presence of NAC (20mM). The stimulatory effect of ROS was detected also at the level of the PAI-1 promoter in endothelial cells transfected with plasmid p800 LUC containing a PAI-1 promoter fragment (+71 to -800). The PAI-1 promoter activity was increased in the presence of ROS, and was suppressed by up to 75% in the presence of antioxidants [1], On the basis of this study we can conclude that reactive oxygen species play an important role in a cytokine-induced activation of PAI-1 expression, and may act as a signal transduction messenger.

Wydawca

-

Rocznik

Tom

07

Numer

4

Opis fizyczny

p.1065-1071,fig.,ref.

Twórcy

  • Department of Molecular and Medical Biophysics, Medical University of Lodz, Lodz, Poland
autor
  • Department of Biochemistry, Medical University of Lodz, Lodz, Poland
  • Department of Biogenic Amines, Polish Academy of Sciences, Lodz, Poland
autor
  • Department of Molecular and Medical Biophysics, Medical University of Lodz, Lodz, Poland

Bibliografia

  • 1. Świątkowska, M., Pawłowska, Z., Szemraj, J. and Cierniewski, C. S. The mechanism of the regulation of PAI-1 gene expression by reactive oxygen species. Cell. Mol. Biol. Lett. 7 (2002) Suppl. 160.
  • 2. Esmon, C. T. Cell mediated events that control blood coagulation and vascular injury. Annu. Rev. Cell. Biol. 9 (1993) 1-26.
  • 3. Matsubara, T. and Ziff, M. Increased superoxide anion release from human endothelial cells in response to cytokines. J. Immunol. 137 (1986) 3295-3298.
  • 4. Sundaresan, M., Yu, Z. X., Ferrans, V. J., Irani, K. and Finkel, T. Requirement for generation of H2O2 for platelet-derived growth factor signal transduction. Science 270 (1995) 298-301.
  • 5. Berk, B. C. Redox signals that regulate the vascular response to injury. Thromb. Haemost. 82 (1999) 810-817.
  • 6. Suzuki, Y. J., Forman, H. J. and Sevanian, A. Oxidants as stimulators of signal transduction. Free Radic. Biol. Med. 22 (1997) 269-285.
  • 7. Palmer, H. J. and Paulson, K. E. Reactive oxygen species and antioxidants in signal transduction and gene expression. Nutr. Rev. 55 (1997) 353-361.
  • 8. Sen, C. K. and Packer, L. Antioxidant and redox regulation of gene transcription. FASEB J. 10 (1996) 709-720.
  • 9. Soeda, S., Tsunoda, T., Kurokawa, Y. and Shimeno, H. Tumor necrosis factor-alpha-induced release of plasminogen activator inhibitor-1 from human umbilical vein endothelial cells: involvement of intracellular ceramide signaling event. Biochim. Biophys. Acta 1448 (1998) 37-45.
  • 10. Świątkowska, M., Cierniewska-Cieślak, A., Pawłowska, Z. and Ciemiewski, C. S. Dual regulatory effects of nitric oxide on plasminogen activator inhibitor type 1 expression in endothelial cells. Eur. J. Biochem. 267 (2000) 1001-1007.
  • 11. Chomczyński, P. and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162 (1987) 156-159.
  • 12. Świątkowska, M., Pawłowska, Z., Szemraj, J., Drzewoski, J., Watala, C. and Cierniewski, C. S. Cerivastatin, a HMG CoA reductase inhibitor, reduces plasminogen activator inhibitor-1 expression in endothelial cells by down- regulation of cellular signalling and the inhibition of PAI-1 promoter activity. Jap. J. Pharm. 90 (2002) (in press).
  • 13. Rahman, A., Kefer, J., Bando, M., Niles, W. D. and Maik, A. B. E-selectin expression in human endothelial cells by TNF-α-induced oxidant generation and NF-κB activation. Am. J. Physiol. Lung Cell Mol. Physiol. 272 (1998) L533-L544.
  • 14. Meier, B., Radeke, H. H., Selle, S., Younes, M., Sies, H., Resch, K. and Habermehl, G. G. Human fibroblasts release reactive oxygen species in response to interleukin-1 or tumor necrosis factor-α. Biochem. J. 263 (1998) 539-545.
  • 15. Hashimoto, S., Gon, Y., Matsumoto, K., Takeshita, I. and Horie, T. N- acetylcysteine attenuates TNF-alpha-induced p38 MAP kinase activation and p38 MAP kinase-mediated IL-8 production by human pulmonary vascular endothelial cells. Br. J. Pharmacol. 132 (2001) 270-276.
  • 16. Świątkowska, M., Pawłowska, Z., Szemraj, J., Drzewoski, J., Watała, C. and Cierniewski, C. S. Cerivastatin, a HMG CoA reductase inhibitor, reduces plasminogen activator inhibitor-1 expression in endothelial cells by down-regulation of cellular signalling and the inhibition of PAI-1 promoter activity. Jap. J. Pharm. 90 (2002) in press.
  • 17. Dechend, R., Maass, M., Gieffers, J., Dietz, R., Scheidereit, C., Leutz, A. and Gulba, D. C. Chlamydia pneumoniae infection of vascular smooth muscle and endothelial cells activates NF-κB and induces tissue factor and PAI-1 expression. Circulation 100 (1999) 1369-1373.
  • 18. Dawson, S. J., Wiman, B., Hamsten, A., Green, F., Humphries, S. and Henney, A. M. The two allele sequences of a common polymorphism in the promoter of the plasminogen activator inhibitor-1 (PAI-1) gene respond differently to interleukin-1 in HepG2 cells. J. Biol. Chem. 268 (1993) 10739-10745.

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Bibliografia

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