Effect of the culture medium and biotic stimulation on taxane production in Taxus globosa Schltdl in vitro cultures

• Autorzy: Tapia N. , Zamilpa A. , Bonfill M. , Ventura E. , Cruz-Vega D. , Del Villar A. , Cruz-Sosa F. , Osuna L.
• Języki publikacji: EN

Abstrakty

EN

Taxus globosa is the only species of the Taxus genus that grows in Mexico. In this study, callus cultures from leaves and young shoots of T. globosa were established in Gamborg’s B5 medium supplemented with 2,4-dichlorophenoxiacetic acid (2 mg/L), kinetin (0.5 mg/L) and gibberellic acid (0.25 mg/L). Callus growth and taxane production were evaluated using two culture media: Woody Plant Medium and Gamborg’s B5 supplemented with picloram (2 mg/L), kinetin (0.1 mg/L) and gibberellic acid (0.5 mg/L). The effect of the inoculum size (50, 100 and 150 g FW/L) and culture media (Woody Plant Medium and Gamborg’s B5) with and without the presence of methyl jasmonate (100 μM) on T. globosa cell suspensions was assessed. Taxane analysis revealed that the calli in Gamborg’s B5 produced taxol (50 μg/g DW), baccatin III, 10-deacetyl baccatin III and 10-deacetyl taxol. Woody Plant Medium also induced the production of taxol, although to a lesser extent. The optimum inoculum size was 50 g FW/L. In cell suspension cultures, both media had a significant effect on taxane production when supplemented with methyl jasmonate. In Woody Plant Medium, at day 14, a total concentration of 197.999 μg/L of taxol, 160.622 μg/L of baccatin III, 633.724 μg/L of 10-deacetyl baccatin III and 229.611 μg/L 10-deacetyl taxol were obtained, with total excretion of baccatin III and 10-deacetyl taxol to the culture medium. In Gamborg’s B5, cephalomanine was obtained at a concentration of 91.428 μg/L without elicitation, and all taxanes were excreted to the medium to a variable extent.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 12
• Opis fizyczny: p.3447-3455,fig.,ref.

Twórcy

autor

Tapia N.

• Division de Ciencias Biologicas y de la Salud, Departamento de Biotecnologıa, Universidad Autonoma Metropolitana-Iztapalapa, 09340 Mexico, DF, Mexico
• Centro de Investigacion Biomedica del Sur. Instituto Mexicano del Seguro Social (IMSS), Argentina 1, Col. Centro, C.P. 62790 Xochitepec, Morelos, Mexico

autor

Zamilpa A.

• Centro de Investigacion Biomedica del Sur. Instituto Mexicano del Seguro Social (IMSS), Argentina 1, Col. Centro, C.P. 62790 Xochitepec, Morelos, Mexico

autor

Bonfill M.

• Facultad de Farmacia, Universidad de Barcelona, Barcelona, Spain

autor

Ventura E.

• Facultad de Farmacia, Universidad de Barcelona, Barcelona, Spain

autor

Cruz-Vega D.

• Centro de Investigacion Biomedica del Noreste. Instituto Mexicano del Seguro Social, Monterrey, Nuevo Leon, Mexico

autor

Del Villar A.

• Facultad de Farmacia, Universidad de Barcelona, Barcelona, Spain

autor

Cruz-Sosa F.

• Division de Ciencias Biologicas y de la Salud, Departamento de Biotecnologıa, Universidad Auto´noma Metropolitana-Iztapalapa, 09340 Mexico, DF, Mexico

autor

Osuna L.

• Centro de Investigacion Biomedica del Sur. Instituto Mexicano del Seguro Social (IMSS), Argentina 1, Col. Centro, C.P. 62790 Xochitepec, Morelos, Mexico

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