Semi-specific PCR for the evaluation of diversity among cultivars of wheat and triticale

• Autorzy: Gawel M. , Wisniewska I. , Rafalski A.
• Języki publikacji: EN

Abstrakty

EN

The usefulness of a semispecific PCR system for the evaluation of genetic diversity among selected cultivars of wheat (Triticum aestivum L.) and triticale (x Triticosecale Wittm.) was investigated. A comparison was made of the diversity among cultivars of both species as revealed by primers targetting the semi-conservative sequences of the intron-exon junction. The main purpose of the study was the selection of the most informative primers from among the exon targetting (ET) and intron targetting (IT) primers of 12 to 18 bases in length. For triticale, the most satisfactory results were obtained using ET and IT primers of 18 bases in length. In wheat, complex and polymorphic banding patterns were generated by primers belonging to both groups, regardless of their length.

Słowa kluczowe

EN

genetic diversity; plant cultivar; wheat; Triticum aestivum; Triticosecale; triticale; bread wheat; polymerase chain reaction

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2002
• Tom: 07
• Numer: 2A
• Opis fizyczny: p.577-582,fig.,ref.

Twórcy

autor

Gawel M.

• Department of Plant Biochemistry and Physiology, Plant Breeding and Acclimatization Institute, Radzikow, 05-870 Blonie, Poland

autor

Wisniewska I.

• Department of Plant Biochemistry and Physiology, Plant Breeding and Acclimatization Institute, Radzikow, 05-870 Blonie, Poland

autor

Rafalski A.

• Department of Plant Biochemistry and Physiology, Plant Breeding and Acclimatization Institute, Radzikow, 05-870 Blonie, Poland

Bibliografia

• 1. Devos, K. M. and Gale, M. D. The use of random amplified polymorphic DNA markers in wheat. Theor. Appl. Genet. 84 (1992) 567-572.
• 2. Brown, J. W. A catalogue of splice junction and putative branch point sequences from plant introns. Nucleic Acids Res. 14 (1986) 9549-9559.
• 3. Rafalski, A., Gidzińska, M. and Wiśniewska, I. PCR-based system for evaluation of relationship among maize inbreds. in: Genetics, Biotechnology and Breeding of Maize and Sorghum, (Tsaftaris, A. S. Ed), Royal Soc. Chem., Cambridge, U. K. 1997, 106-111.
• 4. Davis, L. G., Dibner, M. D. and Battey, J. F. Rapid DNA preparation. in: Basic Methods in Molecular Biology, 1st edition, Elsevier Sci. Publ., New York, 1986, 42-43.
• 5. Rafalski, A., Wiśniewska, I., Adamczyk, J., Gaweł, M. and Królikowski,
• Z. Molecular analysis of genetic diversity among maize inbred lines (in Polish). Biul. IHAR 217 (2001) 127-137.
• 6. Gupta, P. K., Varshney, R. K., Sharma, P. C. and Ramesh, B. Molecular markers and their application in wheat breeding. Plant Breed. 118 (1999) 369-390.
• 7. Li, G. and Quiros, C. F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor. Appl. Genet. 103 (2001) 455-461.