Effect of genetic modification on the health status of transgenic pigs produced with the human α-1,2-fucosyltransferase gene

• Autorzy: Wieczorek J. , Slomski R. , Kowalski W. , Smorag Z.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to evaluate the influence of transgenesis on the animal health status, morbidity rate and course of diseases in a herd of transgenic pigs during 6 years of observation. It was evaluated 378 potentially transgenic pigs with the human α-1,2 fucosyltransferase gene, born after artificial insemination. One of the parents was transgenic and the other non-transgenic. Molecular verification of transgenesis was performed in all piglets. Piglets with positive molecular verification were the experimental group (n = 150), and the other, non-transgenic piglets were the control group (n = 228). In the transgenic group, was diagnosed 43 sick animals (28.7%). Their mortality rate was high: out of the 43 sick animals, 24 (55.81%) died of disease, which accounted for 16.0% of the total population of transgenic animals. Recovery was observed in 19 out of the 43 sick animals (44.19%). Out of 228 non-transgenic animals, 55 suffered from disease (24.12%). Twenty-seven of them (49.9%) died of disease, which constituted 11.5% of the whole non-transgenic population. Twenty-eight animals (50.9%) recovered. Transgenesis had no negative influence on the health, morbidity or mortality of animals. A comparison of transgenic and non-transgenic siblings in the same litters reveals that the observed differences are due to reasons other than transgenesis. The most important potential cause is extensive inbreeding.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2011
• Tom: 67
• Numer: 07
• Opis fizyczny: p.462-466,ref.

Twórcy

autor

Wieczorek J.

• Department of Animal Reproduction Biotechnology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice

autor

Slomski R.

autor

Kowalski W.

autor

Smorag Z.

Bibliografia

• 1.Bao W. B., Wu S. L., Musa H. H., Zhu G. Q., Chen G. H.: Genetic variation at the alpha-1-fucosyltransferase (FUT1) gene in Asian wild boar and Chinese and Western commercial pig breeds. J. Anim. Breed Genet. 2008, 125, 427-430.
• 2.Coddens A., Verdonck F., Mulinge M., Goyvaerts E., Miry C., Goddeeris B., Duchateau L., Cox E.: The possibility of positive selection for both F18(+) Escherichia coli and stress resistant pigs opens new perspectives for pig breeding. Vet. Microbiol. 2008, 126(1-3), 210-215.
• 3.Coddens A., Verdonck F., Tiels P., Rasschaert K., Goddeeris B. M., Cox E.: The age-dependent expression of the F18+ E. coli receptor on porcine gut epithelial cells is positively correlated with the presence of histo-blood group antigens. Microbiology 2007, 122(3-4), 332-341.
• 4.Costache M., Cailleau A., Fernandez-Mateos P., Oriol R., Mollicone R.: Advances in molecular genetics of á-2- and á-3/4-fucosyltransferases. Transfusion Clinique et Biologique 1997, 4(4), 367-382.
• 5.Greger M.: Trait selection and welfare of genetically engineered animals in agriculture. J. Anim. Sci. 2010, 88, 811-814.
• 6.Janowski H., Szweda W., Janowski T. E.: Szczegółowa patologia i terapia chorób świń. Wydawnictwo ART, Olsztyn 1997, pp. 655.
• 7.Jura J., Słomski R., Smorąg Z., Gajda B., Wieczorek J., Lipiński D., Kalak R., Juzwa W., Zeyland J.: Production of transgenic pigs suitable for xenotransplantation with the use of standard DNA microinjection. Ann. Anim. Sci. 2004, 4(2), 321-327.
• 8.Jura J., Smorąg Z., Gajda B., Wieczorek J., Lipiński D., Słomski R.: Uzyskanie transgenicznych świń metodą mikroiniekcji egzogennego DNA. Biotechnologiczne i medyczne podstawy ksenotransplantacji. Część II. Biotechnologiczne podstawy ksenotransplantacji. Z. Smor¹g, R. S³omski, L. Cierpka (ed.), OWN, Poznań 2006, 121-133.
• 9.Klukowska J., Urbaniak B., Świtoński M.: High frequency of M307a mutation at FUT1 locus, causing resistance to oedema disease, in an autochthonous Polish pig breed, the Zlotnicka Spotted. J. Anim. Breed. Genet. 1999, 116(6), 519-524.
• 10.Nagy B., Fekete P. Z.: Enterotoxigenic Escherichia coli in veterinary medicine. International Journal of Medical Microbiology 2005, 295(6-7), 443-454.
• 11.Niemann H., Kues W. A.: Application of transgenesis in livestock for agriculture and biomedicine. Anim. Reprod. Sci. 2003, 79, 291-317.
• 12.Pursel V. G., Pinkert, K. F. Miller, D. J. Bolt, R. G. Campbell, R. D. Palmiter, R. L. Brinster, and R. E. Hammer: Genetic engineering of livestock. Science 1989, 244, 1281-1288.
• 13.Redwan el-R. M.: Animal-derived pharmaceutical proteins. J. Immunoassay Immunochem. 2009, 30(3), 262-290.
• 14.Robl J. M., Wang Z., Kasinathan P., Kuroiwa Y.: Transgenic animal production and animal biotechnology. Theriogenology 2007, 67(1), 127-133.
• 15.Thompson P. B., Hannah W.: Food and Agricultural Biotechnology: A Summary and Analysis of Ethical Concerns. Adv Biochem Engin/Biotechnol. 2008, 111, 229-264.
• 16.Zyśk A.: GMO wciąż budzi obawy. Środowisko 2009, 13/14, 24-25.

Uwagi

Rekord w opracowaniu