The release of sulfur compounds during degradation of feather keratin by two Bacillus strains

• Autorzy: Laba W. , Choinska A. , Rodziewicz A.

Warianty tytułu

PL

Uwalnianie związków siarki podczas degradacji piór przez dwa szczepy Bacillus

• Języki publikacji: EN

Abstrakty

EN

Keratinolytic bacteria Bacillus polymyxa B20 and B. cereus B5esz during cultures in medium with chicken feathers as a sole nutrient source, accumulated various amounts of sulfur compounds at different oxidation level, including thiols, thiosulfate, sulfite and sulfate. The main difference observed between the two tested strains was higher release of sulfate by the former and elevated concentration of thiols by the latter. Additionally, the activity of glutathione reductase, that could potentially play a role in keratinolysis was confirmed, mainly in the cell homogenate fraction, rather than extracellular. Keratinases in crude culture fluids exhibited activity towards soluble keratin preparation, as well as native feather keratin. Application of 2-mercaptoethanol and sulfite, agents that potentially could take part in keratin sulfitolysis, led to a conclusion that they could play a role in keratin degradation, other than activation of extracellular enzymes.

PL

Keratynolityczne bakterie Bacilus polymyxa B20 i B. cereus B5esz podczas wzrostu w podłożu z piórami, jako głównym źródłem składników odżywczych, akumulowały w środowisku znaczną ilość związków siarki na różnym stopniu utlenienia, w tym związki tiolowe, tiosiarczany, siarczyny i siarczany. Podstawową różnicą zaobserwowaną pomiędzy dwoma testowanymi mikroorganizmami było wyższe stężenie siarczanów uwalnianych do podłoża przez bakterie B. polymyxa oraz zdolność bakterii B. cereus do akumulacji w podłożu zredukowanych związków tiolowych. Dodatkowo, aktywność reduktazy glutationowej, mogącej potencjalnie brać udział w procesie keratynolizy, została potwierdzona głównie w homogenacie komórek, w przeciwieństwie do frakcji pozakomórkowej. Keratynazy obecne w płynie pohodowlanym wykazywały aktywność zarówno wobec keratyny rozpuszczalnej, jak i keratyny natywnej. Zastosowanie w mieszaninie reakcyjnej 2-merkaptoetanolu oraz siarczynu pozwoliło zwiększyć aktywność badanych keratynaz poprzez intensyfikację sulfitolizy, natomiast nie na drodze aktywacji tych enzymów.

Słowa kluczowe

EN

sulphur compound; degradation; feather keratin; Bacillus strain; disulphide reductase

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Biotechnologia
• Rocznik: 2013
• Tom: 12
• Numer: 2
• Opis fizyczny: p.29-40,fig.,ref.

Twórcy

autor

Laba W.

• Department of Biotechnology ans Food Microbiology, Wrocław University of Environmental and Life Sciences, Chelmonskiego 37/41, 51-630 Wroclaw, Poland

autor

Choinska A.

• Department of Biotechnology ans Food Microbiology, Wrocław University of Environmental and Life Sciences, Chelmonskiego 37/41, 51-630 Wroclaw, Poland

autor

Rodziewicz A.

• Department of Biotechnology ans Food Microbiology, Wrocław University of Environmental and Life Sciences, Chelmonskiego 37/41, 51-630 Wroclaw, Poland

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