Biotransformation of hydroquinone and 4-hydroxybenzoic acid in Schisandra chinensis (Chinese magnolia vine) in vitro cultures

• Autorzy: Szopa A. , Kwiecien I. , Ekiert H.
• Języki publikacji: EN

Abstrakty

EN

Optimization of the process of biotransformation of hydroquinone into its β-D-glucoside – arbutin, was performed in agitated shoot cultures of Schisandra chinensis. The optimisation involved testing various concentrations of the precursor and different ways of administering it. Arbutin was accumulated mainly in the in vitro cultured biomass (85.2–98.6%). By optimizing the process, a 2.26-fold increase in the overall product content was obtained. The highest amount (17.8 mg·g–1 DW) was found after administering 384 mg·l–1 hydroquinone in a dose divided into two portions. An experiment with the biotransformation of 4-hydro- xybenzoic acid did not produce arbutin but a mixture of two products of glucosylation of the precursor – hydroxybenzoic acid 4-O-β-glucopyranoside and 4-hydroxybenzoic acid β-glucopyranosyl ester. The identity of all biotransformation products was confirmed by 1H-NMR analysis. The results for the production of arbutin by the biotransformation of hydroquinone are of potential practical importance. On the other hand, the fact of confirming the presence of two glucosylation products has a great cognitive value.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2017
• Tom: 16
• Numer: 6
• Opis fizyczny: p.57-66,fig.,ref.

Twórcy

autor

Szopa A.

• Department of Pharmaceutical Botany, Collegium Medicum, Jagiellonian University, Medyczna 9, 30-688 Krakow, Poland

autor

Kwiecien I.

• Department of Pharmaceutical Botany, Collegium Medicum, Jagiellonian University, Medyczna 9, 30-688 Krakow, Poland

autor

Ekiert H.

• Department of Pharmaceutical Botany, Collegium Medicum, Jagiellonian University, Medyczna 9, 30-688 Krakow, Poland

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Identyfikatory

DOI

10.24326/asphc.2017.6.5