Influence of different optical stimulation parameters on strength of depolarization in ChR2-transfected neurons

• Autorzy: Skladowska A. , Bekisz M. , Majkowska M. , Lempicka M. , Kublik E.
• Języki publikacji: EN

Abstrakty

EN

INTRODUCTION: To use optogenetics in well control manner it is necessary to characterize the relation between the light power and resulting opening of light gated channels. AIM(S): To this end we tested the dependence of membrane depolarization on the parameters of light stimulation in channelrhodopsin-transfected neurones in rat’s central nucleus of amygdala (CeA). METHOD(S): Under general anesthesia rats were were injected with AAV-hSyn-ChR2-EYFP viral vector introducing ChR2 to CeA. During in vitro patch-clamp recording on brain slices, we measured the membrane depolarization evoked by a blue light emitted from LED source. Cells were stimulated with trains of light impulses with varying: 1) light power; 2) duration of light impulse; 3) frequency of light impulses. RESULTS: 1) Train of 2 ms light impulses delivered at 20 Hz, a driving current varying from 0.1 to 1 A: relation between light power and membrane depolarization can be approximated by a logarithmic function: 2.2 ln(x)+13 (at the resting potential kept at −50 mV) and 8 ln(x)+28 (at −60 mV). The dependency of the latency of first action potential on the light intensity can be approximated by a power function: 1.2 ×^(−1.3); 2) Train of light impulses of varying duration (range 2–20 ms) at 20 Hz, current 1 A and resting membrane potential kept at −50 mV: the relation between light impulse duration and resulting membrane depolarization can be approximated by a logarithmic function 3 ln(x)+4; 3) Train of 2 ms light impulses with a current set at 1 A and a frequency varying in a range 20–200 Hz (resting potential kept at −50 mV): relation between light impulse frequency and resulting membrane depolarization can be approximated by a logarithmic function: 2.5 ln(x)+3.5. CONCLUSIONS: Our results offers the guideline allowing to estimate expected depolarizing effects of light stimulation on the ChR2-transfected neuronal population in central nucleus of amygdala in rats. FINANCIAL SUPPORT: Supported by Polish National Science Centre grant 2013/08/W/NZ4/00691.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2017
• Tom: 77
• Numer: Suppl.1
• Opis fizyczny: p.136

Twórcy

autor

Skladowska A.

• Department of Neurophysiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Bekisz M.

• Department of Neurophysiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Majkowska M.

• Department of Neurophysiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Lempicka M.

• Department of Neurophysiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland
• Faculty of Physics, Warsaw University of Technology, Warsaw, Poland

autor

Kublik E.

• Department of Neurophysiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland