Heterologous expression of hemagglutinin-neuraminidase protein from Newcastle disease virus strain AF2240 in Centella asiatica

• Autorzy: Lai K.S. , Yusoff K. , Mahmood M.
• Języki publikacji: EN

Abstrakty

EN

We explored the use of the medicinally important plant Centella asiatica for expression of hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) strain AF2240. HN protein is the principal target for subunit vaccine development against NDV. The full-length HN gene was cloned into a plant expression construct driven by the CaMV 35S promoter and C-terminal fusion of green fluorescence protein (GFP) as reporter system. The recombinant expression construct was transformed via particle bombardment into C. asiatica callus. Transformants were screened using GFP and selected on MS medium supplemented with 15 mg/l hygromycin. The ~1.8 kb HN mRNA transcript was detected on the putative transformants using RT-PCR. The presence of HN protein expression was further confirmed through dot blot analysis using anti-NDV chicken serum. Here we report, for the first time, the use of a novel medicinal plant as a new platform for HN protein expression.

Słowa kluczowe

EN

Centella asiatica; antigen expression; edible vaccine; hemagglutinin-neuraminidase; medicinal plant; Newcastle disease virus; heterologous expression

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2012
• Tom: 54
• Numer: 1
• Opis fizyczny: p.142-147,fig.,ref.

Twórcy

autor

Lai K.S.

• Department of Biochemistry, University Putra Malaysia (UPM), 43400 Serdang, Selangor DE, Malaysia

autor

Yusoff K.

autor

Mahmood M.

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