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2012 | 54 | 2 |

Tytuł artykułu

Indirect organogenesis of faba bean (Vicia faba L. minor)

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Using four Polish Vicia faba L. minor cultivars (Bronto, Dino, Tibo, Nadwiślański) we obtained callus from epicotyl fragments collected from 7- and 14-day-old seedlings and from cotyledonary nodes of immature seeds. Callus induction efficiency varied from 81% to 97% depending on the origin of the explant. Shoots regenerated only from the cotyledonary nodes of all tested cultivars. On average, 50% of the explants grown on MS medium containing 1.0 mg dm-3 NAA, 0.5 mg dm-3 BAP, 0.25 mg dm-3 GA3, 1.0 g dm-3 casein hydrolysate, 750 mg dm-3 inositol, 3% sucrose and 0.4% agar were able to regenerate shoots. The number of calluses regenerating shoots was highest from explants collected from fruiting nodes 6 to 9. Decapitation of donor plants increased the percentage of calluses regenerating shoots. On half-strength MS medium with 2 mg dm-3 NAA and on 1/2 MS alone, 11% of the shoots rooted; on 1/2 MS with 1 g dm-3 AC, 8.0% rooted. The regenerants were transferred to Perlite with Hoagland medium and acclimated. Ten percent of the regenerated plants survived the acclimation process, flowered and produced seeds.

Wydawca

-

Rocznik

Tom

54

Numer

2

Opis fizyczny

p.102-108,fig.,ref.

Twórcy

autor
  • The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Cracow, Poland
autor

Bibliografia

  • BAHGAT S, SHABBAN OA, EL-SHIHY O, LIGHTFOOT DA, and ELSHEMY HA. 2008. Establishment of the regeneration systemfor Vicia faba L. Current Issues of MolecularBiology 11 (suppl. 1): 47–54.
  • CIONINI PS, BENNICI A, and D'AMATO F. 1978. Nuclear cytology of callus induction and development in vitro. I. Callusfrom Vicia faba cotyledons. Protoplasma 96: 101–112.DUC G. 1997. Faba bean (Vicia faba L.). Field Crops Research53: 99–109.
  • FAKHRAI H, FAKHRAI F, and EVANS PK. 1989. In vitro culture and plant regeneration in Vicia faba subsp. equina (var. Spring Blaze). Journal of Experimental Botany 40: 813–817.
  • GRANT ME, and FULLER KW. 1968. Tissue culture of root cells of Vicia faba. Journal of Experimental Botany 19: 667–680.
  • GRIGA M. 1988. The study of differences in regeneration potential of various Vicia faba L. genotypes in meristem-tip culture in vitro. Rostlinna Vyroba 34 (6): 613–626.
  • GRIGA M, and KLENOTICOVA H. 1994. Plant regeneration in callus cultures of faba bean (Vicia faba L.). RostlinnaVyroba 40 (8): 697–709.
  • GRIGA M, KUBALAKOVA M, and TEJKLOVA E. 1987. Somatic embryogenesis in Vicia faba L. Plant Cell, Tissue andOrgan Culture 9: 167–171.
  • HAMDY MA, and HATTORI K. 2006a. Regeneration of (Vicia faba L.) cultivars from mature seed cotyledons. AsianJournal of Plant Sciences 5 (4): 623–629.
  • HAMDY MA, and HATTORI K. 2006b. In vitro micropropagation of (Vicia faba L.) cultivars 'Waza soramame' and 'Cairo241' by nodal explants proliferation and somaticembryogenesis. Biotechnology 5(1): 32–37.
  • HAMDY MA, and HATTORI K. 2007. Histological observation on plant regeneration in faba bean cotyledon (Vicia faba L.) cultured in vitro. Asian Journal of Plant Sciences 6 (5):723–731.
  • HOAGLAND DR, and ARNON DI. 1938. A water culture method for growing plants without soil. Circular, UniversityCalifornia, Agricultural Experimental Station No. 347. JELASKA S, PEVALEK B, PAPES D, and DEVIDE Z. 1981.Developmental aspects of long-term callus culture ofVicia faba L. Protoplasma 105: 285–292.
  • KHALAFALLA MM, and HATTORI K. 1999. A combination of thidiazuron and benzyladenine promotes multiple shoot productionfrom cotyledonary node explants of faba bean(Vicia faba L.). Plant Growth Regulation 27: 145–148.
  • MURASHIGE T, and SKOOG F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures.Physiologia Plantarum 15: 473–497.
  • MITCHELL JP, and GILDOW FE. 1975. The initiation and maintenance of Vicia faba tissue cultures. PhysiologiaPlantarum 34: 250–253.
  • PEVALEK B, JELASKA S, PAPES D, and DEVIDE Z. 1980. Growth regulators requirement for the initiation of Vicia fabacallus tissue. Acta Botanica Croatia 39: 51–57.
  • RÖPER W. 1981. Callus formation from protoplasts derived from cell suspension cultures of Vicia faba L. Journal ofPlant Breeding 101: 75–78.
  • SELVA E, STOUFFS M, and BRIQUET M. 1989. In vitro propagation of Vicia faba L. by micro-cutting and multiple shootinduction. Plant Cell, Tissue and Organ Culture 18:167–179.
  • SHIMIZU-SATO S, TANAKA M, and MORI H. 2009. Auxin-cytokinin interactions in the control of shoot branching. PlantMolecular Biology 69: 429–435.
  • TEGEDER M, GEBHARDT D, SCHIEDER O, and PICKARDT T. 1995. Thidiazuron- induced plant regeneration from protoplastsof Vicia faba cv. Mythos. Plant Cell Reports 15:164–169.
  • VANKETESWARAN S. 1962. Tissue culture studies on Vicia faba L. I. Establishment of culture. Phytomorphology 12:300–306.

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