EN
Under current regulations, it is possible to export processed animal proteins originating from tissues of animals other than ruminants. In order to ensure appropriate control, studies were undertaken to adopt a real-time PCR method for the analysis of processed products of animal origin. The material consisted of raw by-products of beef, pork and poultry. In the assays, mitochondrial DNA was used. The modified method is capable of detecting ruminant DNA at a level of 0.025%. The results of validation studies demonstrate that the real-time PCR method can be used in routine tests to identify ruminant DNA in raw animal by-products.