Badania immunobiochemiczne surowic białych szczurów zarażonych doświadczalnie Toxoplasma gondii

• Autorzy: Cegielka M. , Uminski J. , Tos-Luty S.

Warianty tytułu

EN

Studies on immunobiochemical sera of white rats with experimental Toxoplasma gondii infection

RU

Immunobiokhimicheskie issledovanija syvorotok belykh krys ehksperimenyal'no inficirobannykh pri pomoshhi Toxoplasma gondii

• Języki publikacji: PL

Abstrakty

EN

White rats (10) were infected with RH Toxoplasma gondii strain administering a Toxoplasma suspension on 7 occasions at 3-day intervals (2 ml of the peritoneal exudate from infected mice, at the dilution of 1:10). The obtained rat sera gave a positive reaction in the complement fixation test with Toxoplasma antigen (1:10-1:160). The electroproteinograms of immune sera (paper electrophoresis, staining according to Grassman-Hannig, photodensitometric measurements) were found to contain albumins and globulins α₁ and α₂, β, γ; as compared with the sera from non-infected rats there were hyperproteinemia and increase in β- and γ- globulins. The inactivation of sera at 56° did not affect the electroproteinogram pattern. Similar results were obtained by electrophoresis of immune sera on gel agar, with the difference that the γ-globulin fraction was extended to γ₁ and γ₂ subfractions. Immunoelectrophoresis (according to Grabar-Williams' and Scheideger's methods) of immune sera, as compared with those of non-infected rat sera, showed more conspicuous precipitation lines in the zones α₂, β and γ as well as a more clear-cut line corresponding to β₂M subfraction. The inactivated sera: gave a smaller number of precipitation lines. Preliminary studies by microdiffusion and agar gel techniques revealed the presence of 5 precipitation lines in the immune sera and only 3 lines in the normal ones. The inactivation of sera diminished the number of precipitation lines to 2 in immune sera, and to 1 in those of non-infected rats. Quantitative determinations of the fractions are under way.

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 1964
• Tom: 10
• Numer: 4-5
• Opis fizyczny: s.308-310

Twórcy

autor

Cegielka M.

• Zakład Antropozoonoz, Instytut Medycyny Pracy i Higieny Wsi w Lublinie, Lublin
• Zakład Parazytologii Wiejskiej, Instytut Medycyny Pracy i Higieny Wsi w Lublinie, Lublin

autor

Uminski J.

• Zakład Antropozoonoz, Instytut Medycyny Pracy i Higieny Wsi w Lublinie, Lublin
• Zakład Parazytologii Wiejskiej, Instytut Medycyny Pracy i Higieny Wsi w Lublinie, Lublin

autor

Tos-Luty S.

• Zakład Antropozoonoz, Instytut Medycyny Pracy i Higieny Wsi w Lublinie, Lublin
• Zakład Parazytologii Wiejskiej, Instytut Medycyny Pracy i Higieny Wsi w Lublinie, Lublin