Targeting tams-1 gene results in underestimation of Theileria annulata infection in diseased cattle in Egypt

• Autorzy: Ghoneim A.M. , El-Fayomy A. O.
• Języki publikacji: EN

Abstrakty

EN

Tropical theileriosis is considered one of the most economically important tick borne diseases that cause significant mortality and morbidity to livestock. In the context of epidemiological studies on livestock in Egypt, this report investigated the spread of Theileria annulata among diseased farm cows (Bos indicus) over one year. Blood samples collected from 130 cows were investigated by routine staining and 64 samples were investigated by PCR assay using two different probes targeting tams-1 gene. Microscopy revealed the infection of 33.8% of animals with Theileria while PCR detected infection in 51% of animals with one primer pair and the other primer pair detected the infection in 31% of animals. Combined PCR results indicated the infection of 68.8% of animals with T. annulata. Seasonal fluctuation of parasite infection was evident with the highest infection percentage and prevalence recorded during summer based on both microscopy and PCR data. For the first time, the current study reports the presence of two T. annulata isolates based on tams-1 gene partial sequence in Egypt. Targeting polymorphic genes for parasite detection may result in underestimation of infection and target gene diversity has to be considered. The high infection with these pathogens in the clinically ill cows necessitates implementing serious programs to minimize their economic burden on the Egyptian farming industry.

Słowa kluczowe

EN

tams-1 gene; polymerase chain reaction; theileriosis; infection; Theileria annulata; cattle; Bos indicus; Egypt

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2014
• Tom: 59
• Numer: 1
• Opis fizyczny: p.85-90,fig.,ref.

Twórcy

autor

Ghoneim A.M.

• Department of Zoology, Faculty of Science, Damietta University, New Damietta, Damietta, Egypt

autor

El-Fayomy A. O.

• Department of Zoology, Faculty of Science, Damietta University, New Damietta, Damietta, Egypt

Bibliografia

• Adel E.M. 2007. Studies on some blood parasites infecting farm animals in Gharbia Governorate. PhD thesis, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
• Baldwin C.L., Black S.J., Brown W.C., Conrad P.A., Goddeeris B.M., Kinuthia S.W., Lalor P.A., MacHugh N.D., Morrison W.I., Morzaria S.P., Naessens J., Newson J. 1988. Bovine T cells, B cells, and null cells are transformed by the protozoan parasite Theileria parva. Infection and Immunity, 56, 462–467.
• Bilgic H.B., Karagenc T., Shiels B., Tait A., Eren H., Wei, W. 2010. Evaluation of cytochrome b as a sensitive target for PCRbased detection of T. annulata carrier animals. Veterinary Parasitology, 174, 341–347. DOI: 10.1016/j.vetpar.2010.08.025.
• Billiouw M., Brandt J., Vercruysse J., Speybroeck N., Marcotty T., Mulumba M., Berkvens D., 2005. Evaluation of the indirect fluorescent antibody test as a diagnostic tool for East Coast fever in eastern Zambia. Veterinary Parasitology, 127, 189–198. DOI: 10.1016/j.vetpar.2004.09.028.
• Bose R., Jorgensen W.K., Dalgliesh R.J., Friedhoff K.T., De Vos A.J. 1995. Current state and future trends in the diagnosis of babesiosis. Veterinary Parasitology, 57, 61–74. DOI: 10.1016/0304-4017(94)03111-9.
• Burridge M.J., Brown C.G., Kimber C.D. 1974. Theileria annulata: cross reactions between a cell culture schizont antigen and antigens of East African Theileria species in the indirect fluorescent antibody test. Experimental Parasitology, 35, 374–380. DOI: 10.1016/0014-4894(74)90043-5.
• Caeiro V. 1999. General review of tick species present in Portugal. Parassitologia 41, 11–15.
• Callow L.L. 1984. Protozoan and rickettsial diseases. In: Australian Bureau of Animal Health, Animal Health in Australia, Vol. 5. Australian Government Publishing Service, Canberra. pp. 121–216.
• Chaussepied M., Langsley G. 2011. Theileria-induced leukocyte transformation: an example of oncogenic addiction? In: (Eds. Kaufmann S.H.E., Rouse B.T., Sacks D.L.) The Immune Response to Infection. ASM Press, Washington, DC, pp. 537–546.
• Coles G.C. 2001. The future of veterinary parasitology. Veterinary Parasitology, 98, 31–390. DOI: 10.1016/S0304-4017(01)00421-6.
• Dobbelaere D., Rottenberg S. 2003. Theileria-induced leukocyte transformation. Current Opinion in Microbiology, 6, 377–382. DOI: 10.1016/S1369-5274(03)00085-7.
• d’Oliveira C., van der Weide M., Habela M.A., Jacquiet P., Jongejan F. 1995. Detection of Theileria annulata in blood samples of carrier cattle by PCR. Journal of Clinical Microbiology, 33, 2665–9.
• Fawcett D.W., Doxsey S., Stagg D.A., Young A.S. 1982. The entry of sporozoites of Theileria parva into bovine lymphocytes in vitro: electron microscopic observations. European Journal of Cell Biology, 27, 10–21.
• Friedhoff K., Bose R. 1994. Recent developments in diagnostics of some tick-borne diseases. In: (Eds. Uilenberg G., Permin A., Hansen J.W.) Use of applicable biotechnological methods for diagnosing haemoparasites. Proceedings of the Expert Consultation, Merida, Mexico, October 4–6, 1993. Food and Agriculture Organization of the United Nations (FAO), Rome, Italy, pp. 46–57.
• Gamal EI-Dien H.Y. 1993. Studies on Theileria protozoan among cattle in Behera Province. M.V.Sc. thesis, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
• Georges K., Loria G.R., Riili S., Greco A., Caracappa S., Jongejan F., Sparagano O. 2001. Detection of haemoparasites in cattle by reverse line blot hybridization with a note on the distribution of ticks in Sicily. Veterinary Parasitology, 99, 273–286.
• Grootenhuis J.G., Young A.S., Dolan T.T., Stagg D.A. 1979. Characteristics of Theileria species (eland) infections in eland and cattle. Research in Veterinary Science, 27, 59–68.
• Gubbels J.M., de Vos A.P., van der W.M., Viseras J., Schouls L.M., de Vries E., Jongejan F. 1999. Simultaneous detection of bovine Theileria and Babesia species by reverse line blot hybridization. Journal of Clinical Microbiology, 37, 1782–1789.
• Gubbels M.J., Katzer F., Hide G., Jongejan F., Shiels B.R. 2000. Generation of a mosaic pattern of diversity in the major merozoite-piroplasm surface antigen of Theileria annulata. Molecular and Biochemical Parasitology, 110, 23–32. DOI: 10.1016/S0166-6851(00)00253-X.
• Homer M.J., Aguilar-Delfin I., Telford S.R. 3rd, Krause P.J., Persing D.H. 2000. Babesiosis. Clinical Microbiology Reviews, 13, 451–469. DOI: 10.1128/CMR.13.3.451-469.2000.
• Jongejan F., Uilenberg G. 1994. Ticks and control methods. Revue Scientifique et Technique, 13, 1201–1226.
• Kiltz H.H., Uilenberg G., Franssen F.F., Perie N.M. 1986. Theileria orientalis occurs in Central Africa. Research in Veterinary Science, 40, 197–200.
• L’Hostis M., Seegers H. 2002. Tick-borne parasitic diseases in cattle: current knowledge and prospective risk analysis related to the ongoing evolution in French cattle farming systems. Veterinary Research, 33, 599–611. DOI: 10.1051/vetres:2002041.
• Milne M.A. 1950. The ecology of the sheep tick, Ixodes ricinus L. Spatial distribution. Parasitology, 40, 35–45. DOI: 10.1017/S0031182000017832.
• Mohamed A.M., Abdel-Rady A., Ahmed L.S., El-Hosary A. 2012. Evaluation of indirect TaSP enzyme-linked immunosorbent assay for diagnosis of tropical theileriosis in cattle (Bos indicus) and water buffaloes (Bubalus bubalis) in Egypt. Veterinary Parasitology, 86, 486–9. DOI: 10.1016/j.vetpar.2011.11.028.
• Moretti A., Mangili V., Salvatori R., Maresca C., Scoccia E., Torina A., Moretta I., Gabrielli S., Tampieri M.P., Pietrobelli M. 2010. Prevalence and diagnosis of Babesia and Theileria infections in horses in Italy: a preliminary study. Veterinary Journal, 184, 346–350. DOI: 10.1016/j.vetpar.2011.11.028.
• Nayel M., El-Dakhly K.M., Aboulaila M., Elsify A., Hassan H., Ibrahim E., Salama A., Yanai T. 2012. The use of different diagnostic tools for Babesia and Theileria parasites in cattle in Menofia, Egypt. Parasitology Research, 111, 1019–1024. DOI: 10.1016/j.tvjl.2009.03.021.
• Preston P.M. 2001. Theilerioses. In: (Ed. Wallingford M.W.) Encyclopedia of arthropod-transmitted infections of man and domesticated animals. CABI, Wallingford, pp. 487–502.
• Santos M, Soares R, Costa P, Amaro A, Inácio J, Gomes J. 2013. Revisiting the Tams1-encoding gene as a species-specific target for the molecular detection of Theileria annulata in bovine blood samples. Ticks and Tick Borne Diseases, 4, 72–77. DOI: 10.1016/j.ttbdis.2012.07.006.
• Silva M.G., Marques P.X., Oliva A. 2010. Detection of Babesia and Theileria species infection in cattle from Portugal using a reverse line blotting method. Veterinary Parasitology, 174, 199–205. DOI: 10.1016/j.vetpar..2010.08.038.
• Spooner R.L., Innes E.A., Glass E.J., Brown C.G. 1989. Theileria annulata and T. parva infect and transform different bovine mononuclear cells. Immunology, 66, 284–288.