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Czasopismo

2016 | 75 | 2 |

Tytuł artykułu

Morphological characteristics of the sinus node on postmortem tissue

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The sinus node is an intensively researched structure in terms of anatomical, histological, electrophysiological, molecular and genetic approach. For postmortem diagnosis it is still difficult to investigate due to a still reduced accessibility. In this study we tried and succeed to apply molecular biology techniques on postmortem tissues in order to widen the range of postmortem forensic investigation and provide information related to the diagnostic of cardiac arrhythmia. We described the stages of this investigation, with dissection, preservation and analysis that included classical histology, immunohistochemistry, confocal microscope, microdissection, RIN testing, mRNA expression obtaining a precise morphofunctional location of the sinus node. (Folia Morphol 2016; 75, 2: 216–223)

Słowa kluczowe

Wydawca

-

Czasopismo

Rocznik

Tom

75

Numer

2

Opis fizyczny

p.216-223,fig.,ref.

Twórcy

autor
  • Prosecture Department, National Institute of Legal Medicine, Vitan-Barzesti 9, sector 4, 042122 – Bucharest, Romania
autor
  • University of Manchester, Manchester, United Kingdom
autor
  • University of Manchester, Manchester, United Kingdom
autor
  • Prosecture Department, National Institute of Legal Medicine, Bucharest, Romania
  • University of Manchester, Manchester, United Kingdom

Bibliografia

  • 1. Anderson KR, Ho SY, Anderson RH (1979) Location and vascular supply of sinus node in human heart. Br Heart J, 41: 28–32. doi: 10.1136/hrt.41.1.28.
  • 2. Bauer M, Gramlich I, Polzin S, Patzelt D (2003) Quantification of mRNA degradation as possible indicator of postmortem interval: a pilot study. Legal Med, 5: 220–227. doi: 10.1016/j.legalmed.2003.08.001.
  • 3. Bonow RO, Mann DL, Zipes DP, Libby P (2011) Braunwald’s Heart Disease: A Textbook of Cardiovascular Medicine, 2-Volume Set. Saunders, Philadelphia.
  • 4. Boyett MR, Inada S, Yoo S, Li J, Liu J, Tellez J, Greener ID, Honjo H, Billeter R, Lei M, Zhang H, Efimov IR, Dobrzynski H (2006) Connexins in the sinoatrial and atrioventricular nodes. Adv Cardiol, 42: 175–197. doi: 10.1159/000092569
  • 5. Chandler N, Aslanidi O, Buckley D, Inada S, Birchall S, Atkinson A, Kirk D, Monfredi O, Molenaar P, Anderson R, Sharma V, Sigg D, Zhang H, Boyett M, Dobrzynski H (2011) Computer three-dimensional anatomical reconstruction of the human sinus node and a novel paranodal area. Anat Rec, 294: 970–979. doi: 10.1002/ar.21379.
  • 6. Chandler NJ, Greener ID, Tellez JO, Inada S, Musa H, Molenaar P, Difrancesco D, Baruscotti M, Longhi R, Anderson RH, Billeter R, Sharma V, Sigg DC, Boyett MR, Dobrzynski H (2009) Molecular architecture of the human sinus node insights into the function of the cardiac pacemaker. Circulation, 119: 1562–1575. doi: 10.1161/circulationaha.108.804369.
  • 7. Christoffels VM, Smits GJ, Kispert A, Moorman AF (2010) Development of the pacemaker tissues of the heart. Circ Res, 106: 240–254. doi: 10.1161/circresaha.109.205419.
  • 8. Dobrzynski H, Anderson RH, Atkinson A, Borbas Z, D’Souza A, Fraser JF, Inada S, Logantha SJ, Monfredi O, Morris GM, Moorman AF, Nikolaidou T, Schneider H, Szuts V, Temple IP, Yanni J, Boyett MR (2013) Structure, function and clinical relevance of the cardiac conduction system, including the atrioventricular ring and outflow tract tissues. Pharmacol Ther, 139: 260–288. doi: 10.1016/j.pharmthera.2013.04.010.
  • 9. Dobrzynski H, Boyett MR, Anderson RH (2007) New insights into pacemaker activity promoting understanding of sick sinus syndrome. Circulation, 115: 1921–1932. doi: 10.1161/circulationaha.106.61601.
  • 10. González-Herrera L, Valenzuela A, Marchal JA, Lorente JA, Villanueva E (2013) Studies on RNA integrity and gene expression in human myocardial tissue, pericardial fluid and blood, and its postmortem stability. Forensic Sci Int, 232: 218–228. doi: 10.1016/j.forsciint.2013.08.001.
  • 11. Heinrich M, Lutz-Bonengel S, Matt K, Schmidt U (2007) Real-time PCR detection of five different “endogenous control gene” transcripts in forensic autopsy material. Forensic Sci Int Genet, 1:163–169. doi: 10.1016/j.fsigen.2007.01.002.
  • 12. Heinrich M, Matt K, Lutz-Bonengel S, Schmidt U (2007) Successful RNA extraction from various human postmortem tissues. Int J Legal Med, 121: 136–142. doi: 10.1007/s00414-006-0131-9.
  • 13. James TN (1970) Cardiac conduction system: fetal and postnatal development. Am J Cardiol, 25: 213–226. doi: 10.1016/0002-9149(70)90581-3.
  • 14. Keith A, Flack M (1907) The form and nature of the muscular connections between the primary divisions of the vertebrate heart. J Anatom Physiology, 41 (Part 3): 172.
  • 15. Ludwig J (2010) Handbook of autopsy practice. Humana Press Totowa, NJ. http://dx.doi.org/10.1007/978-1-59259-286-9.
  • 16. Michaud K, Romain N, Taroni F, Horisberger B, Mangin P (2002) Evaluation of a simplified method of the conduction system analysis in 110 forensic cases. Forensic Scien Int, ce 130: 13–24. http://dx.doi.org/10.1016/s0379-0738(02)00269-4.
  • 17. Monfredi O, Dobrzynski H, Mondal T, Boyett MR, Morris GM (2010) The anatomy and physiology of the sinoatrial node: a contemporary review. Pacing Clin Electrophysiol, 33: 1392–1406. doi: 10.1111/j.1540-8159.2010.02838.x.
  • 18. Oliva A, Brugada R, D’Aloja E, Boschi I, Partemi S, Brugada J, Pascali VL (2011) State of the art in forensic investigation of sudden cardiac death. Am J Forensic Med Pathol, 32: 1–16. doi: 10.1097/PAF.0b013e3181c2dc96.
  • 19. Partemi S, Berne PM, Batlle M, Berruezo A, Mont L, Riuró H, Ortiz JT, Roig E, Pascali VL, Brugada R, Brugada J, Oliva A (2010) Analysis of mRNA from human heart tissue and putative applications in forensic molecular pathology. Forensic Scien Int, 203: 99–105. http://dx.doi.org/10.1016/j.forsciint.2010.07.005.
  • 20. Partemi S, Coll Vidal M, Campuzano O, Pezzella M, Paravidino R, Pascali VL, Tassinari CA, Striano S, Zara F, Brugada R, Striano P, Oliva A (2013) Importance of post-mortem genetic testing in SUDEP patients. Forensic Scien Int, Genetics Supplement Series, 4: e354–e355. http://dx.doi.org/10.1016/j.fsigss.2013.10.180.
  • 21. Preece P, Cairns NJ (2003) Quantifying mRNA in postmortem human brain: influence of gender, age at death, postmortem interval, brain pH, agonal state and inter-lobe mRNA variance. Mol Brain Res, 118: 60–71. doi: 10.1016/s0169-328x(03)00337-1.
  • 22. Vennemann M, Koppelkamm A (2010) Postmortem mRNA profiling II: Practical considerations. Forensic Scien Int, 203: 76–82. doi: 10.1016/j.forsciint.2010.07.007.
  • 23. Vilallonga JR (2003) Anatomical variations of the coronary arteries: I. The most frequent variations. Eur J Anat, 7: 29–42.
  • 24. Yasojima K, McGeer E, McGeer P (2001) High stability of mRNAs postmortem and protocols for their assessment by RT-PCR. Brain Res Protocols, 8: 212–218. doi: 10.1016/s1385-299x(01)00119-2.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-c8df4e49-646f-4c20-a218-c3dea6e51b67
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