GP5 protein - based ELISA for the detection of PRRSV antibodies

• Autorzy: Wang Y. , Guo J. , Qiuao S. , Li Q. , Yang J. , Jin Q. , Zhang G.
• Języki publikacji: EN

Abstrakty

EN

Porcine reproductive and respiratory syndrome virus (PRRSV) is an important swine pathogen, causing huge economic losses each year worldwide. Immunization with vaccines containing the glycoprotein 5 (GP5) of PRRSV is the main measure to induce neutralizing antibodies and control the disease. Here, we developed a GP5 protein-based ELISA for detecting antibodies against PRRSV. The overall yield of purified GP5 in E. coli flask culture was more than 45 mg/L cell culture. Western blot and IFA indicated that the GP5 protein was highly immunogenic. After optimization and validation with IDEXX PRRS using 566 clinical sera, the DSN, DSP, and accuracy of GP5-ELISA were 81.39%, 75.96%, and 80.39%, respectively. Besides, GP5-ELISA is highly specific, showing no cross-reactions with sera against other important swine pathogens. Hence, GP5 is a good diagnostic antigen and the GP5 protein-based ELISA has the potential to be used in the field

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2016
• Tom: 19
• Numer: 3
• Opis fizyczny: p.495-501,fig.,ref.

Twórcy

autor

Wang Y.

• School of Public Health, Xinxiang Medical University, Xinxiang, 453003, P.R. China
• Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R. China

autor

Guo J.

• Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R. China

autor

Qiuao S.

• Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R. China

autor

Li Q.

• Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R. China

autor

Yang J.

• Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R. China

autor

Jin Q.

• Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R. China

autor

Zhang G.

• College of Veterinary Medicine and Animal Science, Henan Agricultural University, Zhengzhou 450002, P.R. China

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Identyfikatory

ISBN

10.1515/pjvs-2016-0062