Morphological analysis of testicles in cats with disorders of sex development

• Autorzy: Dzimira S. , Nizanski W. , Madej J.A.
• Języki publikacji: EN

Abstrakty

EN

Disorders of sex development (DSD) are rare in cats. They can be caused by chromosomal aberrations, gene mutations or other undefined factors. The aim of the present study was to compare the histological structure and immunohistochemical reactivity of testes in cats with DSD and in healthy cats. The research material consisted of the gonads of four cats – phenotypic males with an incorrect structure of the reproductive system. The control group consisted of the testes of four healthy cats – routinely castrated phenotypical males. The material was fixed with formalin and embedded in paraffin; the sections were stained with hematoxylin and eosin. The immunohistochemical investigation were performed using monoclonal and polyclonal antibodies directed against desmin, vimentin, actin of smooth muscles, S100 protein and MCM3 protein. The results obtained allow concluding that the testes of cats with DSD differed in certain respects, mainly in the number of blood vessels, from the normal testes. Moreover, the results of immunohistochemical examination indicate that in the testes of cats with DSD the number of supporting cells is lower, the amount of interstitial cells is comparable and spermatogenesis is correct es compared to those determined in the control gonads. The number of blood vessels in cats with DSD is reduced by about 30%. It confirms the recommendations for castration of these animals in order to eliminate the potential inheritance of sex development disorders.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2017
• Tom: 20
• Numer: 1
• Opis fizyczny: p.123-131,fig.,ref.

Twórcy

autor

Dzimira S.

• Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wroclaw, Poland

autor

Nizanski W.

• Department of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Plac Grunwaldzki 49, 50-366 Wroclaw, Poland

autor

Madej J.A.

• Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, C.K. Norwida 31, 50-375 Wroclaw, Poland

Bibliografia

• Banco B, Giudice C, Veronesi MC, Gerosa E, Grieco V (2010) An immunohistochemical study of normal and neoplastic canine sertoli cells. J Comp Pathol 143: 239-247.
• Bredal WP, Thoresen SI, Kvellestad A, Lindblad K (1997) Male pseudohermaphroditism in a cat. J Small Anim Pract 38: 21-24.
• Christensen BW (2012). Disorders of sexual development in dogs and cats. Vet Clin North Am Small Anim Pract 42: 515-526.
• Dzimira S, Nizanski W, Ochota M, Madej JA (2015) Histopathological pattern of gonads in cases of sex abnormalities in dogs: an attempt of morphological evaluation involving potential for neoplasia. Pathol Res Pract 211: 772-775.
• Dybdahl Thomsen P, Byskov AG, Basse A (1987) Fertility in two cats with X-chromosome mosaicism and unilateral ovarian dysgenesis. J Reprod Fertil 80: 43-47.
• Haimoto H, Hosoda S, Kato K (1987) Differential distribution of immunoreactive S100-alpha and S100-beta proteins in normal nonnervous human tissues. Lab Invest 57: 489-498.
• Jonhston SD, Buoen LC, Madl JE, Weber AF, Smith FO (1983) X-chromosome monosomy (37,XO) in a Burmese cat with gonadal dysgenesis. J Am Vet Med Assoc 182: 986-989.
• King GJ, Johnson EH (2000) Hypospadias in a Himalayan cat. J Small Anim Pract 41: 508-510.
• Laeman T, Rowland R, Long SE (1999)Male tortoiseshell cats in the United Kingdom. Vet Rec 144: 9-12.
• Meyers-Wallen VN, Wilson JD, Griffin JE, Fisher S, Moorhead PH, Goldschmidt MH, Haskins ME, Patterson DF (1989) Testicular feminization in a cat. J Am Vet Med Assoc 195: 631-634.
• Meyers-Wallen VN (2006) Genetics, genomics and molecular biology of sex determination in small animals. Theriogenology 66: 1655-1658.
• Meyers-Wallen VN (2012) Gonadal and sex differentiation abnormalities of dogs and cats. Sex Dev 6: 46-60.
• Millis DL, Hauptman JG, Johnson CA (1992) Cryptorchidism and monorchidism in cats: 25 cases (1980-1989). J Am Vet Med Assoc 200: 1128-1130.
• Nowacka-Woszuk J, Szczerbal I, Salamon S, Kociucka B, Jackowiak H, Prozorowska E, Slaska B, Rozanska D, Orzelski M, Ochota M, Dzimira S, Lipiec M, Nizanski W, Switonski M (2014) Testicular disorder of sex development in four cats with a male karyotype (38,XY; SRY-positive). Animal Reprod Sci 151: 42-48.
• Poth T, Breuer W, Walter B, Hecht W, Hermanns W (2010) Disorders of sex development in the dog – Adoption of a new nomenclature and reclassification of reported cases. Anim Reprod Sci 121: 197-207.
• ReynoldsBS, Pain A, Meynaud-Collard P,Nowacka-Woszuk J, Szczerbal I, Switonski M, Chastant-Maillard S (2014) Partial urorectal septum malformation sequence in a kitten with disorder of sexual development. J Feline Med Surg 16: 1016-1019.
• Schlafer DH, Valentine B, Fahnestock G, Froenicke L, Grahn RA, Lyons LA, Meyers-Wallen VN (2011) A case of SRY-positive 38,XY true hermaphroditism (XY sex reversal) in a cat. Vet Pathol 48: 817-822.
• Schulman J, Levine SH (1989) Pyometra involving uterusmasculinus in a cat. J Am Vet Med Assoc 194: 690-691.
• Siemieniuch MJ, Wocławek-Potocka I (2007) Morphological features of the seminiferous epithelium in cat (Felis catus, L.1758) testes. J Reprod Dev 53: 1125-1130.
• Switonski M, Szczerbal I, Nizanski W, Kociucka B, Bartz M, Dzimira S, Mikolajewska N (2011) Robertsonian translocation in a sex reversal dog (XX, SRY negative) may indicate that the causative mutation for this intersexuality syndrome resides on canine chromosome 23 (CFA23). Sex Dev 5: 141-146.
• Szczerbal I, Stachowiak M, Dzimira S, Sliwa, K, Switonski M, (2015) The first case of 38,XX (SRY-positive) disorder of sex development in a cat. Mol Cytogenet 8: 22.
• Wilhelm D, Yang JX, Thomas P (2013) Mammalian sex determination and gonad development. Curr Top Dev Biol 106: 89-121.
• Yates D, Hayes G, Heffernan M, Beynon R (2003) Incidence of cryptorchidism in dogs and cats. Vet Rec 152: 502-504.

Identyfikatory

DOI

10.1515/pjvs-2017-0016