Isolation of 2-phenylethanol biosynthesis related genes and their relationship with 2-phenylethanol accumulation in Rosa rugosa

• Autorzy: Feng L. , Wang M. , Wang J. , Zang S. , Xia W. , Sheng L.
• Języki publikacji: EN

Abstrakty

EN

Rosa rugosa is a famous traditional flower in China used not only as a landscape plant but also as a timehonored and valuable aromatic plant. The natural aromatic substance 2-phenylethanol is the major indispensable ingredient of rose flower and rose essential oil. This study adopted R. rugosa ‘Tanghong’ as a test material to isolate 2-phenylethanol biosynthesis related genes RrAADC and RrPAR. The temporal and spatial expression patterns of the two genes in different flower developmental stages and floral organ parts were measured, and the relationship of 2-phenylethanol accumulation to RrAADC and RrPAR expression in R. rugosa was determined. The content of 2-phenylethanol in R. rugosa gradually increased with the degree of flower opening and peaked at the withering stage. The expression level of RrAADC gradually decreased with the degree of flower opening. Meanwhile, the expression level of RrPAR gradually increased from the budding stage to the half opening stage, rapidly decreased at the full opening stage, and slightly increased again toward the withering stage. The content of 2-phenylethanol was the highest in the petals, followed by that in the stamens and pistils. However, this compound was not detected in other parts of the fully opened flower. The expression level of RrAADC peaked in the stamens but was relatively low in the other parts. The expression level of RrPAR was the highest in the stamens and calyxes, followed by that in the pistils and receptacles, and the lowest in the petals and stalks. These results suggest that RrAADC and RrPAR coordinately regulate the biological synthesis of 2-phenylethanol in R. rugosa.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 12
• Opis fizyczny: Article: 256 [9 p.], fig.,ref.

Twórcy

autor

Feng L.

• College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, People’s Republic of China

autor

Wang M.

• College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, People’s Republic of China

autor

Wang J.

• College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, People’s Republic of China

autor

Zang S.

• College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, People’s Republic of China

autor

Xia W.

• College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, People’s Republic of China

autor

Sheng L.

• College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, People’s Republic of China

Bibliografia

• Chen XM, Kobayashi H, Sakai M, Hirata H, Asai T, Ohnishi T, Baldermann S, Watanabe N (2011) Functional characterization of rose phenylacetaldehyde reductase (PAR), an enzyme involved in the biosynthesis of the scent compound 2-phenylethanol. J Plant Physiol 168(2):88–95
• Cheng J, Xie JC, Sun BG (2007) Composition and aromatic characteristics of rose essential oil produced in China. China Food Addit 5:66–70
• Feng LG, Sheng LX, Zhao LY, Yu XY, Shao DW, He XD (2008) Changes of the aroma constituents and contents in the course of Rosa rugosa Thunb. flower development. Sci Agr Sin 41(12):4341–4435
• Feng LG, Chen C, Sheng LX, Liu P, Tao J, Su JL, Zhao LY (2010) Comparative analysis of headspace volatiles of Chinese Rosa rugosa. Molecules 15:8390–8399
• Feng LG, Chen C, Li TL, Wang M, Tao J, Zhao DQ, Sheng LX (2014) Flowery odor formation revealed by differential expression of monoterpene biosynthesis genes and monoterpene accumulation in rose (Rosa rugosa Thunb.). Plant Physiol Bioch 75:80–88
• Hayashi S, Yagi K, Ishikawa T, Kawasaki M, Asai T, Picone J, Turnbull C, Hiratake J, Sakata K, Takada M, Ogawa K, Watanabe N (2004) Emission of 2-phenylethanol from its b-Dglucopyranoside and the biogenesis of these compounds from [2H8] L-phenylalaninein rose flowers. Tetrahedron 60(33):7005–7013
• Hirata H, Ohnishi T, Ishida H, Tomida K, Sakai M, Hara M, Watanabe N (2012) Functional characterization of aromatic amino acid aminotransferase involved in 2-phenylethanol biosynthesis in isolated rose petal protoplasts. J Plant Physiol 169(5):444–451
• Hua DL, Xu P (2011) Recent advances in biotechnological production of 2-phenylethanol. Biotechnol Adv 29:654–660
• Li MR (2010) Changes of aroma components and some related enzymes acitivity in Tree Peony petal during florescence and flower. Master Dissertation, Henan University of Science and Technology
• Ma XH, Wang YH, Wei Q, Zhang GJ (2004) Study on processing technology of rose essential oil. Chem Ind For Prod 24:80–84
• Negre F, Kish CM, Boatright J, Underwood B, Shibuya K, Wagner C, Clark DG, Dudareva N (2003) Regulation of methylbenzoate emission after pollination in snapdragon and petunia flowers. Plant Cell 15(12):2992–3006
• Sakai M, Hirata H, Sayama H, Sekiguchi K, Itano H, Asai T, Dohra H, Hara M, Watanabe N (2007) Production of 2-phenylethanol in roses as the dominant floral scent compound from L-phenylalanine by two key enzymes, a PLP-dependent decarboxylase and a phenylacetaldehyde reductase. Biosci Biotechnol Biochem 71(10):2408–2419
• Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nat Protoc 36:1101–1108
• Tieman D, Taylor M, Schauer N, Fernie AR, Hanson AD, Klee HJ (2006) Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde. PNAS 103:8287–8292
• Tieman DM, Loucas HM, Kim JY, Clark DG, Klee HJ (2007) Tomato phenylacetaldehyde reductases catalyze the last step in the synthesis of the aroma volatile 2-phenylethanol. Phytochemistry 68(21):2660–2669
• Verdonk JC, Ric de Vos CH, Verhoeven HA, Haring MA, van Tunen AJ, Schuurink RC (2003) Regulation of floral scent production in petunia revealed by targeted metabolomics. Phytochemistry 62(6):997–1008
• Watanabe S, Hayashi K, Yagi K, Asai T, MacTavish H, Picone J, Turnbull C, Watanabe N (2002) Biogenesis of 2-phenylethanol in rose flowers: incorporation of [2H8] L-phenylalanine into 2-phenylethanol and its b-D-glucopyranoside during the flower opening of Rosa ‘Hoh-Jun’ and Rosa damascene Mill. Biosci Biotechnol Biochem 66(5):943–947
• Wu LE, Liu W, Pu J, Li LX, Li GP (2012) Analysis on the volatile components from Rhododendron parvifolium in Shangri-La County by GC-MS. Chem Res Appl 24(6):921–925
• Xiang L, Chen LQ (2009) Adavances in genetic engineering of floral scent. Sci Agr Sin 42(6):2076–2084
• Xie C, Wang JH, Gong ZL (2008) Analysis on aromatic constitrents of fragrant flower [Chimonanthus praecox (Linn.) Link] in aroma releasing. J Tea Sci 28(4):282–288
• Xuan XQ, Bo YC, Xu RY, Zhang JS (2007) Studies on analysis of flavor constituents of different maturity tobacco leaves. Chinese Agr Sci Bull 23(2):98–102
• Yang ZY, Sakaia M, Sayamab H, Shimenob T, Yamaguchib K, Watanabea N (2009) Elucidation of the biochemical pathway of 2-phenylethanol from shikimic acid using isolated protoplasts of rose flowers. J Plant Physiol 166(8):887–891
• Yang X, Cheng F, Li JM (2010) Research progress on 2-phenylethanol biosynthesis in plants. Acta Hortic Sin 37(10):1690–1694
• Yang X, Li HJ, Chen F, Yang LL, Zheng JC, Li JM (2011) Inquiry on synthesis mechanism of vidal grape characteristic aroma component-2-phenylethanol. J Chinese Inst Food Sci Technol 11(6):193–198
• Yu Z, Yi YF, Wu Y, Yu XJ, Wang P, Ding JK (1994) The comparison of the chemical constituents and the odour of four rose oils. Acta Botanica Yunnaniea 1:75–80
• Yu YL, Wang QY, Yao L (2012) Research on the aroma constituents and contents of Rosa rugosa ‘Purple Branch’. J Shanghai Jiaotong Univ (Agr Sci) 29(2):80–87
• Zhao XB, Tang YQ, Liu TM (2011) Research progress of p-phenylethanol. China Brew 8:1–4
• Zhou W, Zhou XP, Zhao GH, Liu HW, Ding L, Chen LR (2002) Studies of aroma components on essential oil of chinese Kushui rose. Chin J Chromatogr 6:561–564

Identyfikatory

DOI

10.1007/s11738-015-1996-3