High efficiency method to obtain supercoiled DNA with a commercial plasmid purification kit

• Autorzy: Carbone A. , Fioretti F. M. , Fucci L. , Ausio J. , Piscopo M.
• Języki publikacji: EN

Abstrakty

EN

Supercoiled state corresponds to the active form for plasmid applications. The relaxed circular form of plasmids is often inactive or poorly active. To obtain significant amounts of almost fully supercoiled DNA, we modified the standard protocol of a commercially; available Qiagen plasmid purification kit. Our changes led to isolation of almost 100% of the plasmids in the supercoiled state. The modified protocol was used to purify different plasmids with consistent results. The purified plasmids maintain supercoiled state for about two months. The modified protocol is very advantageous because it allows easy DNA production with high degree of supercoiled form at low cost.

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2012
• Tom: 59
• Numer: 2
• Opis fizyczny: p.275-278,fig.,ref.

Twórcy

autor

Carbone A.

• Department of Structural and Functional Biology, University of Naples Federico II, Naples, Italy

autor

Fioretti F. M.

autor

Fucci L.

autor

Ausio J.

autor

Piscopo M.

Bibliografia

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Uwagi

PL

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