Organ-specific effects of the auxin herbicide 2,4-D on the oxidative stress and senescence-related parameters of the stems of pea plants

• Autorzy: McCarthy-Suarez I. , Gomez M. , del Rio L. A. , Palma J. M.
• Języki publikacji: EN

Abstrakty

EN

Oxidative stress and senescence have been shown to participate in the toxicity mechanism of auxin herbicides in the leaves and roots of sensitive plants. However, their role in stem toxicity has not been studied yet. In this work, we report the effect of foliar or root applications of the auxin herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) on the parameters of oxidative stress and senescence of stems of pea (Pisum sativum L.) plants. Contrary to their effect on the pea leaves, in the stems 2,4-D applications did not cause oxidative stress, as shown by the parameters of lipid peroxidation, protein carbonyls, and total and protein thiols. Moreover, they inhibited the superoxide radical (O₂⁻)-producing xanthine oxidase (XOD) activity and stimulated the antioxidant activities of catalase (CAT), guaiacol peroxidase (GPOX), ascorbate peroxidase (APX), glutathione reductase (GR), glutathione S-transferase (GST) and Krebs cycle NAD⁺-isocitrate dehydrogenase (IDH). Applications of 2,4-D also did not induce senescence in the pea stems, as shown by the increase of proteins, the lack of stimulation of proteolytic activity, and the inhibition of senescence-related isocitrate lyase (ICL) activity. However, they stimulated the H₂O₂-producing acyl-CoA oxidase (ACOX) activity of fatty acid beta oxidation. Results suggest that oxidative stress and senescence are not involved in the mechanism of toxicity of 2,4-D in the stems of pea plants, and that these phenomena are not whole-plant toxicity mechanisms for auxin herbicides in susceptible plants. Results also suggest that the effect of 2,4-D on the oxidative metabolism of pea plants might be organ-specific.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 6
• Opis fizyczny: p.2239-2247,fig.,ref.

Twórcy

autor

McCarthy-Suarez I.

• Departamento de Bioquimica, Biologia Celular y Molecular de Plants, Estacion Experimental del Zaidin, CSIC, Apartado 419, 18080 Granada, Spain
• Departamento de Biologica del Desarrollo, Instituto de Biologia Molecular y Celular de Plantas, CSIC-Universidad Politecnica de Valencia, c/Ingeniero Fausto Elio s/n, 46022 Valencia, Spain

autor

Gomez M.

• Departamento de Bioquimica, Biologia Celular y Molecular de Plants, Estacion Experimental del Zaidin, CSIC, Apartado 419, 18080 Granada, Spain

autor

del Rio L. A.

• Departamento de Bioquimica, Biologia Celular y Molecular de Plants, Estacion Experimental del Zaidin, CSIC, Apartado 419, 18080 Granada, Spain

autor

Palma J. M.

• Departamento de Bioquimica, Biologia Celular y Molecular de Plants, Estacion Experimental del Zaidin, CSIC, Apartado 419, 18080 Granada, Spain

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