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Czasopismo

2012 | 3 |

Tytuł artykułu

Wpływ beta-glukanu (Leiber ®Beta-S) na nieswoiste humoralne mechanizmy obronne u narybku karpia (Cyprinus carpio)

Warianty tytułu

EN
Influence of ß-glucan (Leiber ®Beta-S) on nonspecific humoral defense mechanisms in fingerling carp, Cyprinus carpio L.

Języki publikacji

PL

Abstrakty

EN
Nutritional support is important for optimum health in fish as it provides the building blocks of innate defense mechanisms and thus protection against infectious diseases. In the present study, we determined the influence of β-glucan (Leiber ®Beta-S) delivered with food on the nonspecific humoral defense mechanisms in fingerling carp. The fish weighed 15-17 g. Four tanks in a recirculation system were stocked with 100 fish each. The water temperature was 20-22°C. The fish were fed daily with commercial pellets containing β-glucan (Leiber ®Beta-S) at doses of 100, 200, and 400 mg per kg of pellets. The feed was prepared by the carp protocol used at the Inland Fisheries Institute. The control group was fed glucan-free pellets. On days 14 and 30 of the experiment, blood was drawn from 10 fish from each group and the nonspecific humoral defense mechanism parameters were examined. The results showed that β -glucan (Leiber ®Beta-S) at doses of 100, 200, and 400 mg per kg of pellets increased the myeloperoxidase activity in blood leukocytes and lysozyme activity in the serum. Total protein and immunoglobulin (Ig) levels were also increased in the serum. The optimal stimulating effect was observed following 30 days of application at a dose of 200 mg of β-glucan (Leiber ®Beta-S) on kg of pellets in fingerling carp.

Wydawca

-

Czasopismo

Rocznik

Numer

3

Opis fizyczny

s.14-17,rys.,bibliogr.

Twórcy

autor
  • Zakład Patologii i Immunologii Ryb, Instytut Rybactwa Śródlądowego w Olsztynie, Olsztyn
autor
autor
autor
autor

Bibliografia

  • Bricknell I., Dalmo R.A. 2005 - The use of immunostimulats in fish larval aquaculture - Fish Shellfish Immunol. 19: 457-472.
  • Brown G„ Gordon S. 2003 - Fungal β-Glucans and mammalian immunity - Immunity 19: 311-315.
  • Chen J., Seviour R. 2007 - Medicinal importance of fungal β -(1,3-1,6)-glucans - Mycological Research 111: 635-652.
  • Lull C., Wichers H.J., Savelkoul H.F.J. 2005 - Antiinflamatory and immunomodulating properties of fungal metabolites - Mediators of Inflammation 2: 63-80.
  • Małaczewska J., Wójcik R., Jung L., Siwicki A.K. 2010 - Effect of Biolex β-HP on selected parameters of specific and non-specific humoral and cellular immunity in rats - Bull. Vet. Inst. Pulawy 54: 75-80.
  • Sadula J., Kogan G., Kacurkowa M., Machova E. 1999 - Microbial 1,3-D-glucans, their preparation, physico-chemical characterization and immunomodulatory activity - Carbohydrate Polymers 8: 247-253.
  • Sakai M. 1999 - Current research status of fish immunostimulants - Aquaculture 172: 63-92.
  • Silke C.J., Rohn S., Kroch L.W., Kurz T. 2007 - In vitro potential antioxidant activity of -1,3-1,6-D-glucan and protein fractions from Saccharomyces cerevisiae cell walls - Agric. Food. Chem. 55: 4710-4716.
  • Siwicki A.K. 2011 - Important infectious diseases of common carp (Cyprinus carpio L.) - Training Network on Protective Immune Modulation in Warm Water Fish by Feeding Glucans, PAN Gołysz: 11-15.
  • Siwicki A.K., Anderson D.P., Antychowicz J. 1993a - Nonspecific defence mechanisms assay in fish I. Phagocytic index, adherence and phagocytic ability of neutrophils (NBT test) and myeloperoxidase activity test - Fish Disease Diagnosis and Preventions Methods. Wyd. IRS Olsztyn: 95-103.
  • Siwicki A.K., Anderson D.P. 1993b - Nonspecific defence mechanisms assay in fish II. Potential killing activity of neutrophils and monocytes, lysozyme activity in serum and organs and total immunoglobulin (Ig) level in serum - Fish Disease Diagnosis and Preventions Methods. Wyd. IRS Olsztyn: 105-111.
  • Siwicki A.K., Kazuń K., Lepa A., Kazuń B. 2011 - Influence of 1,3-1,6-D-glucan (Leiber Beta-S) in diets on the effectiveness of anti-Enteric Redmouth Disease (AquaVac ERM) vaccine in rainbow trout (Oncorhynchus mykiss) - Centr. Eur. J. Immunol. 36: 212-214.
  • Siwicki A.K., Lepa A., Terech-Majewska E., Kazuń K., Kazuń B., Głąbski E. 2012 - Flerpeswirus karpia -3 (CyHV-3): aktualny stan wiedzy oraz wpływ CyHV-3 na nieswoiste humoralne mechanizmy obronne karpia (Cyprinus carpio L.) - Komun. Ryb. 2: 11-16.
  • Siwicki A.K., Morand M., Klein P. 1998 - Modulation of nonspecific defence mechanisms and protection against diseases in fish - Acta Vet. Brno 67: 323-328.
  • Siwicki A.K., Zakęś Z., Trapkowska S., Terech-Majewska E., Czerniak S., Głąbski E., Kazuń K. 2003 - Nonspecific cellular and humoral defence mechanisms in pikeperch (Sander lucioperca) grown in a intensive system of culture - Arch. Pol. Fish. 11: 207-212.
  • Siwicki A.K., Zakęś Z., Terech-Majewska E., Kazuń K„ Lepa A., Głąbski E. 2009 - Dietary Macrogard reduces Aeromanas hydrophila mortality in tench (Tinca tinca) through the activation of cellular and humoral defence mechanisms - Rev. Fish Biol. Fisheries DOI 10.1007/sl 1160-009-9133-2.
  • Tsipali E., Whaley S., Kalbfleisch J., Ensley H.E., Browder W., Williams D.L. 2001 - Glucans exhibit weak antioxidant activity, but stimulate macrophage free radical activity - Free Rad. Biol .Med. 30: 393-402.
  • Wójcik R., Janowska E., Mataczewska J., Siwicki A.K. 2009 - Effect of β-1,3/1,6-D-glucan on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in rats - Bull. Vet. Inst. Pulawy 53: 241-246.
  • Wójcik R. 2010 - Effect of Biolex Beta-HP on phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in rats intoxicated by cyclophosphamide - Pol. J. Vet. Sciences 13: 181-188.
  • Yadav M., Schorey J. 2006 - The beta-glucan receptor dectin-1 functions together with TLR2 to mediate macrophage activation by mycobacteria - Blood 108: 3168-3176.

Typ dokumentu

Bibliografia

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Identyfikator YADDA

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