Photosynthetic down-regulation in N2 -fixing alfalfa under elevated CO2 alters rubisco content and decreases nodule metabolism via nitrogenase and tricarboxylic acid cycle

• Autorzy: Erice G. , Sanz-Saez A. , Aroca R. , Ruiz-Lozano J.M. , Avice J.-C. , Irigoyen J.J. , Sanchez-Diaz M. , Aranjuelo I.
• Języki publikacji: EN

Abstrakty

EN

Although responsiveness of N₂-fixing plants to elevated CO₂ conditions have been analyzed in previous studies, important uncertainties remain in relation to the effect enhanced CO₂ in nodule proteomic profile and its implication in leaf responsiveness. The aim of our study was to deepen our understanding of the relationship between leaf and nodule metabolism of N₂-fixing alfalfa plants after longterm exposure to elevated CO₂. After 30-day exposure to elevated CO₂, plants showed photosynthetic down-regulation with reductions in the light-saturated rate of CO₂ assimilation (Asat) and the maximum rate of rubisco carboxylation (Vcmax). Under elevated CO₂ conditions, the rubisco availability limited potential photosynthesis by around 12 %, which represented the majority of the observed fall in Vcmax. Photosynthetic down-regulation has been associated with decreased N availability even if those plants are capable to assimilate N₂. Diminishment in shoot N demand (as reflected by the lower rubisco and leaf N content) suggests that the lower aboveground N requirements affected negatively nodule performance. In this condition, specific nodule activity was reduced due to an effect on nodule metabolism that manifested as a lower amount of nitrogenase reductase. Moreover, the nodule proteomic approach also revealed that nodule functioning was altered simultaneously in various enzyme quantity apart from nitrogenase. At elevated CO₂, the tricarboxylic acid cycle was also altered with a reduced amount of isocitrate synthase protein. The nodule proteome analysis also revealed the relaxation of the antioxidant system as shown by a decline in the amount of catalase and isoflavone reductase protein.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 10
• Opis fizyczny: p.2607-2617,fig.,ref.

Twórcy

autor

Erice G.

• Departamento de Biología Vegetal, Seccion Biología Vegetal, Unidad Asociada al CSIC, EEAD, Zaragoza e ICVV, Logrono, Facultades de Ciencias y Farmacia, Universidad de Navarra, c/Irunlarrea 1, Pamplona, Navarra, Spain

autor

Sanz-Saez A.

• Departament de Biologia Vegetal, Facultat de Biologia, Universidad de Barcelona, Av. Diagonal, 645, 08028, Barcelona, Spain

autor

Aroca R.

• Departamento de Microbiología del Suelo y Sistemas Simbioticos, Estacion Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008, Granada, Spain

autor

Ruiz-Lozano J.M.

• Departamento de Microbiología del Suelo y Sistemas Simbioticos, Estacion Experimental del Zaidín (CSIC), Profesor Albareda 1, 18008, Granada, Spain

autor

Avice J.-C.

• INRA, UMR INRA-UCBN 950 Ecophysiologie Végétale, Agronomie & nutritions N.C.S., Institut de Biologie Fondamentale et Appliquee, IFR 146 ICORE, Esplanade de la Paix, 14000, Caen, France

autor

Irigoyen J.J.

• Departamento de Biología Vegetal, Seccion Biología Vegetal, Unidad Asociada al CSIC, EEAD, Zaragoza e ICVV, Logrono, Facultades de Ciencias y Farmacia, Universidad de Navarra, c/Irunlarrea 1, Pamplona, Navarra, Spain

autor

Sanchez-Diaz M.

• Departamento de Biología Vegetal, Seccion Biología Vegetal, Unidad Asociada al CSIC, EEAD, Zaragoza e ICVV, Logrono, Facultades de Ciencias y Farmacia, Universidad de Navarra, c/Irunlarrea 1, Pamplona, Navarra, Spain

autor

Aranjuelo I.

• Instituto de Agrobiotecnología, Universidad Publica de Navarra-CSIC-Gobierno de Navarra, Campus de Arrosadía, 31192, Mutilva Baja, Spain

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Identyfikatory

DOI

10.1007/s11738-014-1631-8