Controlling of HUCB-NSC culture by micropatterned biofunctionized surfaces

• Autorzy: Zychowicz M. , Buzanska L. , Ruiz A. , Coecke S. , Rossi F. , Domanska-Janik K.
• Języki publikacji: EN

Abstrakty

EN

Stem cells have potential to maintain in organism by self-renewal division and ability to multilineage differentiation. Cell-cell contacts, paracrine signals and extracellular matrix proteins occurring in the neurogenic niches are main determinants of neural stem cells fate and dynamic of their differentiation. Aim of this study is to investigate whether could nano/micro-patterned, biofunctionalized surface guides human cord blood derived neural stem cell to growth and differentiation. Methods: To investigate the infl uence of the cell plating density we used the microcontact printed patterns of adhesive substrate (poly-L-lysine) on cell-repellent poly-ethylene glycol (PEG) substrate. Two different geometries of the patterning have been applied: to test proliferative response the cells were seeded in different densities on the pattern with separated pitches, while to verify their ability to differentiation the culture medium was supplemented with cAMP and cells seeded on the surface patterned with interconnecting lines. After 2, 4 and 7 days the cells were fi xed and immunostained for Ki67 (proliferation marker) and beta III tubulin/ S100β markers for neuronal/astrocytic lineage. Results: The low cell density of HUCB-NSC (104 cell/cm2 ) and the presence of neuromorphogenes (cAMP) supports neural stem cell differentiation, while enhanced initial cell density promotes the growth rate (increase of the cell number falling on biofunctionalized unit per 100 μm2 surface). Conclusions: Micropatterned platforms with biofunctionalized surface can be used for screening of the plethora of extracellular signals directing neural stem cell to growth and differentiation.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 1
• Opis fizyczny: p.105

Twórcy

autor

Zychowicz M.

• NeroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland

autor

Buzanska L.

• NeroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland
• Joint Research CentreC, IHCP, European Commmission, Ispra, Italy

autor

Ruiz A.

• Joint Research CentreC, IHCP, European Commmission, Ispra, Italy

autor

Coecke S.

• Joint Research CentreC, IHCP, European Commmission, Ispra, Italy

autor

Rossi F.

• Joint Research CentreC, IHCP, European Commmission, Ispra, Italy

autor

Domanska-Janik K.

• NeroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland