Expression and immunological evaluation of elongation factor Tu of Streptococcus suis serotype 2

• Autorzy: Xia X.J. , Wang L. , Cheng L.K. , Shen Z.Q. , Li S.G. , Wang J.L.
• Języki publikacji: EN

Abstrakty

EN

Streptococcus suis serotype 2 (SS2) is considered as a major pathogen that causes sepsis and meningitis in piglets and humans, but knowledge of its antigenic proteins remains limited so far. The surface-related proteins of pathogens often play significant roles in bacterium-host interactions and infection. Here, we obtained the elongation factor Tu (EF-Tu) gene of Streptococcus suis and constructed the recombinant expression plasmid successfully. The target recombinant plasmid was then expressed in Escherichia coli and the immuno-protection of the recombinant protein was subsequently evaluated as well. The EF-Tu gene of Streptococcus suis is 1197 bp in length, encodes 398 amino acids. The target recombinant EF-Tu (rEF-Tu) protein can recognize the antiserum of Streptococcus suis and can provoke obvious humoral immune responses in rabbits and conferred protection to rabbits against Streptococcus suis ear-vein challenge, implying that the EF-Tu may be used as an attractive candidate antigen for a component of subunit vaccine.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2017
• Tom: 20
• Numer: 2
• Opis fizyczny: p.277-284,fig.,ref.

Twórcy

autor

Xia X.J.

• College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, NO. 90, Hualan road, Xinxiang, Henan, 453003, P.R.China

autor

Wang L.

• College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, NO. 90, Hualan road, Xinxiang, Henan, 453003, P.R.China

autor

Cheng L.K.

• Shandong Binzhou Animal Science and Veterinary Medicine Academy, No.169, Huanghe 2th road, Lvdu High-Tech Park, Binzhou, Shandong, 256600, China

autor

Shen Z.Q.

• Shandong Binzhou Animal Science and Veterinary Medicine Academy, No.169, Huanghe 2th road, Lvdu High-Tech Park, Binzhou, Shandong, 256600, China

autor

Li S.G.

• Shandong Binzhou Animal Science and Veterinary Medicine Academy, No.169, Huanghe 2th road, Lvdu High-Tech Park, Binzhou, Shandong, 256600, China

autor

Wang J.L.

• Shandong Binzhou Animal Science and Veterinary Medicine Academy, No.169, Huanghe 2th road, Lvdu High-Tech Park, Binzhou, Shandong, 256600, China

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Identyfikatory

DOI

10.1515/pjvs-2017-0033