Ecorehabilitation and biochemical studies of Ceropegia bulbosa Roxb.: a threatened medicinal succulent

• Autorzy: Dhir R. , Shekhawat G.S.
• Języki publikacji: EN

Abstrakty

EN

In the present endeavour, a propagation protocol has been standardized for two varieties of threatened medicinal succulent, Ceropegia bulbosa Roxb. (varieties bulbosa and lushii). The manuscript focuses on morphogenic response of shoot tips when cultured on MS media augmented with growth regulators. Maximum shoot length and highest number of shoots were observed when 6-benzyladenine (8.88 μM) and α-naphthalene acetic acid were used in combination. During multiplication, antioxidant enzymes have shown variation in their activity. Total antioxidant activity and phenolic content were higher in the in vitro plants. Chlorophyll and protein content was higher in in vivo plants while proline was higher in in vitro plants. Superoxide dismutase activity was higher in naturally grown plants while, activities of catalase, glutathione reductase and peroxidase were elevated in the in vitro plants. Isolated cerpegin (pyridine alkaloid) was characterized using thin layer chromatography and attenuated total reflectance spectroscopy.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 06
• Opis fizyczny: p.1335-1343,fig.,ref.

Twórcy

autor

Dhir R.

• Department of Bioscience and Biotechnology, Banasthali University, 304022 Rajasthan, India

autor

Shekhawat G.S.

• Department of Botany, Jai Narain Vyas University, Jodhpur, 342001 Rajasthan, India

Bibliografia

• Abbasi BH, Khan M, Guo B, Bokhari SA, Khan MA (2011) Efficient regeneration and antioxidative enzyme activities in Brassica rapa var. turnip. Plant Cell Tiss Organ Cult 105:337–344
• Adibatti NA, Thirugnanasambantham P, Kulothungan C, Viswanathan S, Kameswaran L, Balakrishna K, Sukumar E (1991) A Pyridine alkaloid from Ceropegia juncea. Phytochemistry 30:2449–24501
• Aebi H (1974) Catalases. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, Verlag Chemie, pp 673–684
• Ahmad N, Javed SB, Khan I, Anis M (2013) Rapid plant regeneration and analysis of genetic fidelity in micropropagated plants of Vitex trifolia: an important medicinal plant. Acta Physiol Plant 35:2493–2500
• Ananthan R, Latha M, Ramkumar KM, Pari L, Baskar C, Narmatha BV (2004) Modulatory effects of Gymnema montanum leaf extract on alloxan induced oxidative stress in wistar rats. Nutrition 20:280–285
• Arnon DL (1949) A copper enzymes in isolated chloroplasts: polypheno oxidase in Beta vulgaris. Plant Physiol 24:1–15
• Avetisyan A, Karapetyan L (2010) Convenient synthesis of Cerpegin. Synth Commun 40(17):2607–2609
• Bates LS, Wadern RP, Teare ID (1973) Rapid estimation of free proline for water stress determination. Plant Soil 39:205–207
• Batkova P, Pospisilova J, Synkova H (2008) Production of reactive oxygen species and development of antioxidative systems during in vitro growth and ex vitro transfer. Biol Plant 52:413–422
• Beena MR, Martin KP, Kirti PB, Hariharan M (2003) Rapid in vitro propagation of medicinally important Ceropegia candelabrum. Plant Cell Tiss Organ Cult 72:285–289
• Benson EE, Lynch PT, Jones J (1992) Variation in free-radical damage in rice cell-suspensions with different embryogenic potentials. Planta 188:296–305
• Beuchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an acrylamide gels. Anal Biochem 44:276–287
• Borthakur A, Das SC, Kalita MC, Sen P (2011) In vitro plant regeneration from apical buds of Albizzia odoratissima (L.f.) Benth. Adv App Sci Res 2(5):457–464
• Britto SJ, Natarajan E, Arockiasamy DI (2003) In vitro flowering and shoot multiplication from nodal explants of Ceropegia bulbosa Roxb. var. bulbosa. Taiwania 48(2):106–111
• Chance B, Maehly AC (1995) Assay of catalases and peroxidises. Methods Enzymol 2:764–775
• Dhaka N, Kothari SL (2005) Micropropagation of Eliptica alba (L.) Hassk-An important medicinal plant. In Vitro Cell Dev Biol Plant 41:658–661
• Dhir R, Shekhawa GS (2013) Production, storability and morphogenic response of alginate encapsulated axillary meristems and genetic fidelity evaluation of in vitro regenerated Ceropegia bulbosa: a pharmaceutically important threatened plant species. Ind Crops Prod 47:139–144
• Dhir R, Shekhawat GS (2012) Critical review on Tecomella undulata: a medicinally potent endangered plant species of Indian Thar Deseart. Int J Curr Res 4(6):36–44
• Dutta Gupta S, Datta S (2003) Antioxidant enzyme activities during in vitro morphogenesis of gladiolus and the effect of application of antioxidants on plant regeneration. Biol Plant 47:179–183
• Faisal M, Anis M (2007) Regeneration of plants from alginate encapsulated shoots of Tylophora indica (Burm.f.) Merrill, an endangered medicinal plant. J Hortic Sci Biotechnol 82:351–354
• Giri L, Jugran A, Rawat S, Dhyani P, Andola H, Bhatt ID, Rawal RS, Dhar U (2012) In vitro propagation, genetic and phytochemical assessment of Habenaria edgeworthii: an important Astavarga plant. Acta Physiol Plant 34(3):869–875
• Gupta SD, Ibaraki Y, (2006) Evaluation of photosynthetic capacity in micropropagated plants by image analysis. In: Gupta and Ibaraki (ed) Plant tissue culture engineering, Springer, Netherlands, pp 15–29
• Hong H, Comins DL (1996) A three-step synthesis of cerpegin. J Org Chem 61:391–392
• Huang CL, Hsieh MT, Hsieh WC, Sagare AP, Tsay HS (2000) In vitro propagation of Limonium wrightii (Hance) Ktze. (Plumbaginaceae), an ethnomedicinal plant from shoot tip, leaf and inflorescence-node explants. In Vitro Cell Dev Biol Plant 36:220–224
• Jain M, Bhandari A, Bhandari A, Patel P (2012) Characterization and in vitro antiurolithiatic activity of cerpegin alkaloid from Ceropegia bulbosa var. Lushii root. Int J Drug Dev Res 4(4):154–160
• Jana S, Shekhawat GS (2012) In vitro regeneration of Anethum graveolens, antioxidative enzymes during organogenesis and RAPD analysis for clonal fidelity. Biol Plant 56(1):9–14
• Khan MA, Pradhan D (2012) Antiurolithc activity of Ceropegia bulbosa extract in rats. Der Pharmacia Sinica 3(1):148–152
• Kirtikar KR, Basu BD (1975) Indian medicinal plants. Bishen Singh Mahendra Pal Singh, Dehradun
• Kumaran A, Karunakaran JR (2007) In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. Food Sci Technol 40(2):344–352
• Kwon YI, Vattem DA, Shetty K (2006) Clonal herbs of lamiaceae species against diabetes and hypertension. Asia Pac J Clin Nut 15:107–118
• Lowry OH, Rosenberg NJ, Farr AL, Randall RJ (1951) Protein measurement with folin phenol reagent. J Biol Chem 193:265–275
• Mabberley DJ (1978) The plant book. Cambridge University Press, Cambridge
• Malik SK, Chaudhury R, Kalia RK (2005) Rapid in vitro multiplication and conservation of Garcinia indica: a tropical medicinal tree species. Sci Hortic 106:539–553
• Marquele FD, Di Mambro VM, Georgetti SR, Casagrande R, Yara ML, Valim YML, Fonseca MJV (2005) Assessment of the antioxidant activities of Brazilian extracts of propolis alone and in topical pharmaceutical formulations. J Pharm Biomed Anal 39:455–462
• Meratan AA, Ghaffari SM, Niknam V (2009) In vitro organogenesis and antioxidant enzymes activity in Acanthophyllum sordidum. Biol Plant 53:5–10
• Mishra J, Rawat B, Agnihotri RK, Chandra A, Nautiyal S (2012) In vitro propagation, genetic and secondary metabolite analysis of Aconitum violaceum Jacq. Acta physiol Plant 35(8):2589–2599
• Mitrovic A, Janosevic D, Budimir S, Pristov JB (2012) Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis. Biol Plant 56(2):357–361
• Molassiotis AN, Dimassi K, Diamantidis G, Therios I (2004) Changes in peroxidases and catalase activity during in vitro rooting. Biol Plant 48:1–5
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
• Nadkarni KM (1976) Indian materia medica. Popular Prakashan, Bombay
• Nakanishi K (1962) Infrared absorption spectroscopy. Holden-Day, San Francisco
• Obert B, Benson EE, Millam S, Pretovb A, Bremner DH (2005) Moderation of morphogenetic and oxidative stress responses in flax in vitro cultures by hydroxynonenal and desferrioxamine. J Plant Physiol 162:537–547
• Patil VM (1998) Micropropagation of Ceropegia spp. In Vitro Cell Dev Biol Plant 34:240–243
• Phulwaria M, Shekhawat NS, Rathore JS, Singh RP (2013) An efficient in vitro regeneration and ex vitro rooting of Ceropegia bulbosa Roxb.—A threatened and pharmaceutical important plant of Indian Thar Desert. Ind Crops Prod 42:25–29
• Piovan A, Caniato R, Mariella Cappelletti E, Filippini R (2010) Organogenesis from shoot segments and via callus of endangered Kosteletzkya pentacarpos (L.) Ledeb. Plant Cell Tiss Organ Cult 100:309–315
• Raja B, Pugalendi KV (2010) Evaluation of antioxidant activity of Melothria maderaspatana in vitro. Cent Eur J Biol 5(2):224–230
• Rathore MS, Chikara J, Shekhawat NS (2011) Plantlet regeneration from callus cultures of selected genotype of Aloe vera L.—An ancient plant for modern herbal industries. Appl Biochem Biotechnol 163:860–868
• Ravishankara MN, Shrivastava N, Padh H, Rajani M (2002) Evaluation of antioxidant properties of root bark of Hemidesmus indicus R. Br. (Anantmul). Phytomedicine 9:153–160
• Roy S, Sehgal R, Padhy BM, Kumar VL (2005) Antioxidant and protective effect of latex of Calotropis procera against alloxan-induced diabetes in rats. J Ethnopharmacol 102:470–473
• Sahai A, Shahzad A (2013) High frequency in vitro regeneration system for conservation of Coleus forskohlii: a threatened medicinal herb. Acta Physiol Plant 35:473–481
• Sahai A, Shahzad A, Anis M (2010) High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica (Burm.f.) Merr., an endangered plant species. Turk J Bot 34:11–20
• Sarasan V, Cripps R, Ramsay MM, Atherton C, McMichen M, Prendergast G, Rowntree JK (2006) Conservation in vitro of threatened plants—progress in the past decade. In Vitro Cell Dev Biol Plant 42:206–214
• Schaedle M, Bassham JA (1997) Chloroplast glutathione reductase. Plant Physiol 59:1011–1012
• Sen A, Batra A (2013) The study of in vitro and in vivo antioxidant activity and total phenolic content of Phyllanthus amarus schum. & thonn.: a medicinally important plant. Int. J Pharm Pharm Sci 5(3):942–947
• Shekhawat MS, Shekhawat NS (2011) Micropropagation of Arnebia hispidissima (Lehm). DC. and production of alkannin from callus and cell suspension culture. Acta Physiol Plant 33: 1445–1450
• Shekhawat GS, Mathur S, Batra A (2009) Role of phytohormones and nitrogen in somatic embryogenesis induction in cell culture derived from leaflets of Azadirachta indica. Biol Plant 53(4):707–710
• Skálová D, Navrátilová B, Richterová L, Knitl M, Sochor M, Vašut RJ (2012) Biotechnological methods of in vitro propagation in willows (Salix spp.). Cent Eur J Biol 7(5):931–940
• Sukumar E, Hamsaveni GR, Bhima RR (1996) Pharmacological actions of cerpegin, a novel pyridine alkaloid from Ceropegia juncea. Fitoterapia 66:403–406
• Swarna J, Ravindhran R (2012) In vitro propagation and assessment of genetic integrity of Talinum triangulare (Jacq.) Willd: a valuable medicinal herb. Acta Physiol plant 34:1987–1996
• Thakar J, Bhargava S (1999) Seasonal variation in antioxidant enzymes and the sprouting response of Gmelina arborea Roxb. nodal sectors cultured in vitro. Plant Cell Tiss Organ Cult 59:181–187
• Tripathi M, Kumari N (2010) Micropropagation of a tropical fruit tree Spondias mangifera Willd. through direct organogenesis. Acta Physiol Plant 32:1011–1015
• Villemin D, Cheikh N, Liao L, Bar N, Lohier JF, Sopkova J, Choukchou-Braham N, Mostefa-Kara B (2012) Two versatile routes towards cerpegin and analogues: applications of a one pot reaction to new analogues of cerpegin. Tetrahedron 68: 4906–4918
• Walter SK, Gillet J (1998) 1997 IUCN Red list of threatened plants. World conservation monitring centre, Cambridge, UK
• Wang J, Gao W, Zhang L, Huang L (2013) Establishment and quality assessment of tissue cultures in Glycyrrhiza uralensis Fisch. Appl Biochem Biotechnol 169:588–594
• Yang YL, Zhang YY, Lu J, Zhang H, Liu Y, Jiang Y, Shi RX (2012) Exogenous H₂O₂ increased catalase and peroxidase activities and proline content in Nitraria tangutorum callus. Biol Plant 56(2):330–336
• Zayova E, Stancheva I, Geneva M, Petrova M, Vasilevska-Ivanova R (2012) Morphological evaluation and antioxidant activity of in vitro- and in vivo-derived Echinacea purpurea plants. Cent Eur J Biol 7(4):698–707

Identyfikatory

DOI

10.1007/s11738-014-1512-1