Influence of selected stool concentration techniques on the effectiveness of PCR examination in Giardia intestinalis diagnostics

• Autorzy: Stojecki K. , Sroka J. , Karamon J. , Kusyk P. , Cencek T.
• Języki publikacji: EN

Abstrakty

EN

Giardia intestinalis is a widespread parasitic protozoa which has great significance as a public health threat. Molecular diagnostics of stool sample can be unreliable because of the presence of inhibitors of enzymatic reactions. The aim of this study was to determine the effectiveness of selected pre-treatment methods of fecal samples for further PCR-based diagnostics of G. intestinalis, and the effect of each component of pre-treatment solutions on PCR reactions. Seven stool concentration techniques were compared. The results showed that the most efficient concentration method for stool sample preparation for detection of G. intestinalis by PCR is centrifugal flotation with Percoll (with saturated NaNO₃ as the flotation solution). This method is relatively inexpensive, less labor-intensive, and suitable for epidemiological monitoring and clinical investigations.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2014
• Tom: 17
• Numer: 1
• Opis fizyczny: p.19-25,ref.

Twórcy

autor

Stojecki K.

• Department of Parasitology and Invasive Diseases, National Veterinary Research Institute in Pulawy, Al.Partyzantow 57, 24-100 Pulawy, Poland

autor

Sroka J.

• Department of Parasitology and Invasive Diseases, National Veterinary Research Institute in Pulawy, Al.Partyzantow 57, 24-100 Pulawy, Poland

autor

Karamon J.

• Department of Parasitology and Invasive Diseases, National Veterinary Research Institute in Pulawy, Al.Partyzantow 57, 24-100 Pulawy, Poland

autor

Kusyk P.

• Department of Parasitology and Invasive Diseases, National Veterinary Research Institute in Pulawy, Al.Partyzantow 57, 24-100 Pulawy, Poland

autor

Cencek T.

• Department of Parasitology and Invasive Diseases, National Veterinary Research Institute in Pulawy, Al.Partyzantow 57, 24-100 Pulawy, Poland

Bibliografia

• Adam RD (2001) Biology of Giardia lamblia. Clin Microbiol Rev 14: 447-475.
• Allen AV, Ridley DS (1970) Further observations on the formol-ether concentration technique for faecal parasites. J Clin Pathol 23: 545-546.
• Al-Soud WA, Rådström P (1998) Capacity of nine thermostable DNA polymerases to mediate DNA amplification in the presence of PCR-inhibiting samples. Appl Environ Microbiol 64: 3748-3753.
• Al-Soud WA, Rådström P (2000) Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat. J Clin Microbiol 38: 4463-4470.
• Ash LR, Orihel TC, Savioli L (1994) Bench aids for the diagnosis of intestinal parasites, 1st ed., World Health Organization, Geneva.
• Bajer A, Bednarska M, Paziewska A, Romanowski J, Siński E (2008). Semi-aquatic animals as a source of water contamination with Cryptosporidium and Giardia. Wiadomości Parazytologiczne 54: 315-318.
• Berkman DS, Lescano AG, Gilman RH, Lopez SL, Black MM (2002) Effects of stunting, diarrhoeal disease, and parasitic infection during infancy on cognition in late childhood: a follow-up study. Lancet 359: 564-571.
• Bukhari Z, Smith HV (1995) Effect of three concentration techniques on viability of Cryptosporidium parvum oocysts recovered from bovine feces. J Clin Microbiol 33: 2592-2595.
• Clavel A, Arnal A, Sanchez E, Varea M, Quilez J, Ramirez I, Castillo FJ (1996) Comparison of 2 centrifugation procedures in the formalin-ethyl acetate stool concentration technique for the detection of Cryptosporidium oocysts. Int J Parasitol 26: 671-672.
• Favre N, Rudin W (1996) Salt-dependent performance variation of DNA polymerases in co-amplification PCR. Biotechniques 21: 28-30.
• Feng Y, Xiao L (2011) Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. Clin Microbiol Rev 24: 110-140.
• Fülleborn F (1920) Die Anreicherungen der Helmintheneier mit Kochsalzlösung. Dtsch Med Wochenschr 46: 714-715.
• Guy RA, Payment P, Krull UJ, Horgen PA (2003) Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage. Appl Environ Microbiol 69: 5178-5185.
• Karamon J, Ziomko I, Cencek T, Sroka J (2008) Modified flotation method with the use of Percoll for the detection of Isospora suis oocysts in suckling piglet faeces. Vet Parasitol 156: 324-328.
• Lalle M, Jimenez-Cardosa E, Cacció SM, Pozio E (2005) Genotyping of Giardia duodenalis from humans and dogs from Mexico using a β-giardin nested polymerase chain reaction assay. J Parasitol 91: 203-205.
• Lane S, Lloyd D (2002) Current trends in research into the waterborne parasite Giardia. Crit Rev Microbiol 28: 123-147.
• Lebbad M, Mattsson JG, Christensson B, Ljungström B, Backhans A, Andersson JO, Sva¨rd SG (2010) From mouse to moose: multilocus genotyping of Giardia isolates from various animal species. Vet Parasitol 168: 231-239.
• Monteiro L, Bonnemaison D, Vekris A, Petry KG, Bonnet J, Vidal R, Cabrita J, Mégraud F (1997) Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model. J Clin Microbiol 35: 995-998.
• Nantavisai K, Mungthin M, Tan-ariya P, Rangsin R, Naaglor T, Leelayoova S (2007) Evaluation of the sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalis in stool specimens. J Clin Microbiol 45: 581-583.
• Nichols RA, Moore JE, Smith HV (2006) A rapid method for extracting oocyst DNA from Cryptosporidium-positive human faeces for outbreak investigations. J Microbiol Methods 65: 512-524.
• Parija SC, Srinivasa H (1999) Viewpoint: the neglect of stool microscopy for intestinal parasites and possible solutions. Trop Med Int Health 4: 522-524.
• Pertoft H, Laurent TC, Låås T, Kågedal L (1978) Density gradients prepared from colloidal silica particles coated by polyvinylpyrrolidone (Percoll). Anal Biochem 88: 271-282.
• Platts-Mills JA, Operario DJ, Houpt ER (2012) Molecular diagnosis of diarrhea: current status and future potential. Curr Infect Dis Rep 14: 41-46.
• Plutzer J, Ongerth J, Karanis P (2010) Giardia taxonomy, phylogeny and epidemiology: Facts and open questions. Int J Hyg Environ Health 213: 321-333.
• Read CM, Monis PT, Thompson RC (2004) Discrimination of all genotypes of Giardia duodenalis at the glutamate dehydrogenase locus using PCR-RFLP. Infect Genet Evol 4: 125-130.
• Robertson LJ, Hanevik K, Escobedo AA, Mzrch K, Langeland N (2010) Giardiasis-why do the symptoms sometimes never stop? Trends Parasitol 26: 75-82.
• Schrader C, Schielke A, Ellerbroek L, Johne R (2012) PCR inhibitors-occurrence, properties and removal. J Appl Microbiol 113: 1014-1026.
• Schuurman T, Lankamp P, Van Belkum A, Kooistra-Smid M, Van Zwet A (2007) Comparison of microscopy, real-time PCR and a rapid immunoassay for the detection of Giardia lamblia in human stool specimens. Clin Microbiol Infect 13: 1186-1191.
• Solarczyk P, Werner A, Majewska AC (2010) Genotype analysis of Giardia duodenalis isolates obtained from humans in West-central Poland. Wiadomości Parazytologiczne 56: 171-177.
• Stauffer SH, Birkenheuer AJ, Levy MG, Marr H, Gookin JL (2008) Evaluation of four DNA extraction methods for the detection of Tritrichomonas foetus in feline stool specimens by polymerase chain reaction. J Vet Diagn Invest 20: 639-641.
• Verweij JJ, Schinkel J, Laeijendecker D, van Rooyen MA, van Lieshout L, Polderman AM (2003) Real-time PCR for the detection of Giardia lamblia. Mol Cell Probes 17: 223-225.
• Weber R, Bryan RT, Juranek DD (1992) Improved stool concentration procedure for detection of Cryptosporidium oocysts in fecal specimens. J Clin Microbiol 30: 2869- -2873.
• Webster KA, Smith HV, Giles M, Dawson L, Robertson LJ (1996) Detection of Cryptosporidium parvum oocysts in faeces: Comparison of conventional coproscopical methods and the polymerase chain reaction. Vet Parasitol 61: 5-13.
• Willis HH (1921) A simple levitation method for the detection of hookworm ova. Med J Aust 8: 375-376.
• Zygner W, Jaros D, Skowrońska M, Bogdanowicz-Kamirska M, Wedrychowicz H (2006) Prevalence of Giardia intestinalis in domestic dogs in Warsaw. Wiadomości Parazytologiczne 52: 311-315.

Identyfikatory

DOI

10.2478/pjvs-2014-0003