In vivo effect of 2-deoxy-D-glucose on glucose-6-phosphate dehydrogenase activity in the cytosol of liver, heart and skeletal muscle of rats

• Autorzy: Torlinska T. , Ozegowski S. , Paluszak J. , Hryniewiecki T.
• Języki publikacji: EN

Abstrakty

EN

Torlińska T., Ożegowski S., Paluszak J., and Hryniewiecki T.: In vivo effect of 2-deoxy-D-glucose on glucose-6-phosphate dehydrogenase activity in the cytosol of liver, heart and skeletal muscle of rats. Acta Physiol. Pol. 1990, 41 (6): 137-143. 2-deoxy-D-glucose (2-DG), the unmetabolizable analogue of glucose induces a series of metabolic, hormonal and behavioral responses, causing cellular glucoprivation. According to in vitro studies, 2-DG inhibits phosphofructokinase in cultured human cells. The present investigations deal with changes in the cytosolic glucocse-6-phosphate dehydrogenase activity following in vivo 2-DG administration. A single dose of 2-DG (600 mg/kg) has no influence on the activity of glucose-6-phosphate dehydrogenase in the cytosol of liver, heart and skeletal muscle of the rat. The concommitant increase in serum glucose, lactate and FFA concentrations observed in the study indicates indirectly a stimulation of adrenergic system. After three days of successive administration of 2-DG to rats, dehydrogenase activity decreased in the liver by approx 57% and in the skeletal muscle by approx 82% in comparison with control animals. Moreover the in vivo effect of 2-DG was found to be fully reversible, probably when the total amount of the inhibitor was excreted.

Słowa kluczowe

EN

in vivo effect; 2-deoxy-D-glucose; glucose-6-phosphate; dehydrogenase activity; cytosol; liver; heart; skeletal muscle; rat

• Wydawca: -
• Czasopismo: Acta Physiologica Polonica
• Rocznik: 1990
• Tom: 41
• Numer: 7
• Opis fizyczny: p.138-145,fig.,ref.

Twórcy

autor

Torlinska T.

• Chair and Department of Physiology, Poznan Academy of Medicine, Swiecickiego 6, 60-781 Poznan, Poland

autor

Ozegowski S.

• Chair and Department of Physiology, Poznan Academy of Medicine, Swiecickiego 6, 60-781 Poznan, Poland

autor

Paluszak J.

• Chair and Department of Physiology, Poznan Academy of Medicine, Swiecickiego 6, 60-781 Poznan, Poland

autor

Hryniewiecki T.

• Chair and Department of Physiology, Poznan Academy of Medicine, Swiecickiego 6, 60-781 Poznan, Poland

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