Phylogenetic analysis of steroid 21-hydroxylase (CYP21) of the wolf and selected breeds of dogs in connection with the role of this enzyme in the pathogenesis of many diseases in the Canidae family

• Autorzy: Kosowska B. , Strzala T. , Brzezinska K. , Grabowski K. , Moska M. , Dobosz T. , Lebioda A.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to determine the nucleotide sequence of the CYP21 gene in the wolf and representatives of five breeds of dogs (selected according to a classification by Parker et al, 2004) in connection with the key role of the product of this gene in the genesis of many diseases in dogs. Nuclear DNA of dogs was obtained from peripheral blood, and the wolf’s DNA was isolated from muscle tissue. The amplification of the 21HS gene was carried out in 10 fragments under standardized PCR conditions. The reaction products were sequenced. The sequence of amino acids in the protein was determined on the basis of the nucleotide sequence. The sequences obtained in our study and those retrieved from the GeneBank database were compared with the Mafft program (15) and subjected to phylogenetic analysis with the MrBayes 3.2 program (35). We detected a total of nine SNP mutations in introns and exons. Furthermore, a deletion of two nucleotides, that differentiates the breeds, was detected in the promoter region. Only two differences between the dogs and the wolf were found in SNP: one in an exon and one in an intron. Genetic distance was determined between the selected breeds of dogs and between the wolf and the dogs of each breed. In addition, we estimated the evolutionary distances between amino acid sequences of the dog/wolf and homologous 21HS sequences of eight different vertebrate species obtained from GeneBank. It was shown that, among mammals, the amino acid sequence of the dog/wolf is the most similar to the sequence of the pig, and the least similar to that of the human. The sequences determined in this study may provide a reference point for the research on the CYP21 gene structure and expression in various tissues of dogs for therapeutic purposes.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2012
• Tom: 68
• Numer: 09
• Opis fizyczny: p.550-556,fig.,ref.

Twórcy

autor

Kosowska B.

• Workshop of Cell Molecular Biology, Department of Genetics, Wroclaw University of Environmental and Life Sciences, ul. Kozuchowska 7, 51-631 Wroclaw, Poland

autor

Strzala T.

autor

Brzezinska K.

autor

Grabowski K.

autor

Moska M.

autor

Dobosz T.

autor

Lebioda A.

Bibliografia

• 1.Barg E., Tokarska M., Wikiera B., Kosowska B.: Congenital Adrenal Hyperplasia - Advances in Diagnosis. Adv. Clin. Exp. Med. 2003, 12, 507-515.
• 2.Cameron P. U., Tabarias H. A., Pulendran B., Robinson W., Dawkins R. L.: Conservation of the central MHC genome: PFGE mapping and RFLP analysis of complement, HSP70, and TNF genes in the goat. Immunogenetics 1990, 31, 253-264.
• 3.Cerundolo R., Lloyd D. H., Vaessen M. M., Mol J. A., Kooistra H. S., Rijnberk A.: Alopecia in pomeranians and miniature poodles in association with high urinary corticoid: Creatinine ratios and resistance to glucocorticoid feedback. Vet. Record. 2007, 160, 393-397.
• 4.Chung B. C., Matteson K. J., Miller W. L.: Structure of a bovine gene for P-450c21 (steroid 21-hydroxylase) defines a novel cytochrome P-450 gene family. Proc. Natl. Acad. Sci. USA 1986, 4, 211-219.
• 5.Corneli P. S.: Complete Mitochondrial Genomes and Eutherian Evolution. J. Mammal. Evol. 2002, 9, 281-305.
• 6.Crawford R. J., Hammond V. E., Connel J. M., Coghlan J. P.: The structure and activity of two cytochrome P450c21 proteins encoded in the ovine adrenal cortex. J. Biol. Chem. 1992, 267, 16212-16218.
• 7.Darriba D., Taboada G. L., Doallo R., Posada D.: ProtTest 3: fast selection of best-fit models of protein evolution. Bioinformatics 2011, 27, 1164-1165.
• 8.Frank L. A.: Growth hormone-responsive alopecia in dogs. J. Am. Vet. Med. Assoc. 2005, 226, 1494-1497.
• 9.Frank L. A., Hnilica K. A., Oliver J. W.: Adrenal steroid hormone concentrations in dogs with hair cycle arrest (Alopecia X) before and during treatment with melatonin and mitotane. Vet. Dermatol. 2004, 15, 278-284.
• 10.Galac S., Kool M. M., Naan E. C., Daminet S., Mol J. A., Kooistra H. S.: Expression of the ACTH receptor, steroidogenic acute regulatory protein, and steroidogenic enzymes in canine cortisol-secreting adrenocortical tumors. Domest. Anim. Endocrinol. 2010, 39, 259-267.
• 11.García-Moreno J., Matocq M. D., Roy M. S., Geffen E., Wayne R. K.: Relationships and genetic purity of the endangered Mexican wolf based on analysis of microsatellite loci. Conserv. Biol. 1996, 10, 376-389.
• 12.Hall T. A.: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids. Symp. Ser. 1999, 41, 95-98.
• 13.Higashi Y., Yoshioka H., Yamane M., Gotoh O., Fujii-Kuriyama Y.: Complete nucleotide sequence of two steroid-hydroxylase genes tandemly arranged in human chromosome: a pseudogene and a genuine gene. Proc. Natl. Acad. Sci. USA 1986, 83, 2841-2845.
• 14.Huang H. P., Lien J. H., Chang P. H.: Effect of Castration on Hair Re-growth in Pomeranians with Hair Cycle Arrest (Alopecia X). JVCS 2009, 1, 17-19.
• 15.Katoh K., Toh H.: Parallelization of the MAFFT multiple sequence alignment program. Bioinformatics 2010, 26, 1899-1900.
• 16.Kawaguchi H., Golubic M., Figueroa F., Klein J.: Organization of the chimpanzee C4-CYP21 region: implications for the evolution of human genes. Eur. J. Immunol. 1990, 20, 739-745.
• 17.Kawaguchi H., Klein J.: Organization of C4 and Cyp21 loci in gorilla and orangutan. Hum. Immunol. 1992, 33, 153-162.
• 18.Kayes-Wandover K. M., White P. C.: Steroidogenic enzyme gene expression in the human heart. J. Clin. Endocrinol. Metab. 2000, 85, 2519-2525.
• 19.Klein S. C., Peterson M. E.: Canine hypoadrenocorticism: part I. Can. Vet. J. 2010, 51, 63-69.
• 20.Lajic S., Eidsmo L., Holst M.: Steroid 21-hydroxylase in the kidney: demonstration of levels of messenger RNA which correlate with the level of activity. J. Steroid Biochem. Mol. Biol. 1995, 52, 181-186.
• 21.Lessard C. J., Ice J. A., Adrianto I., Wiley G. B., Kelly J. A., Gaffney P. M., Montgomery C. G., Moser K. L.: The genomics of autoimmune disease in the era of genome-wide association studies and beyond. Autoimmun. Rev. 2012, 11, 267-275.
• 22.Lindblad-Toh K., Wade C. M., Mikkelsen T. S., Karlsson E. K., Jaffe D. B., Kamal M., Clamp M., Chang J. L., Kulbokas E. J. 3rd, Zody M.C., et al.: Genome sequence, comparative analysis and haplotype structure of the domestic dog. Nature 2005, 438, 803-819.
• 23.Mausberg E. M., Drögemüller C., Dolf G., Rüfenacht S., Welle M., Leeb T.: Exclusion of patched homolog 2 (PTCH2) as a candidate gene for alopecia X in Pomeranians and Keeshonden. Vet. Rec. 2008, 163, 121-123.
• 24.Mausberg E. M., Drögemüller C., Leeb T., Dolf G., Rüfenacht S., Welle M.: Evaluation of the CTSL2 gene as a candidate gene for alopecia X in Pomeranians and Keeshonden. Anim. Biotechnol. 2007, 18, 291-296.
• 25.Mausberg E. M., Drögemuller C., Rüfenacht S., Welle M., Roosje P., Suter M., Leeb T.: Inherited alopecia X in Pomeranians. Dtsch. Tierärztl. Wochenschr. 2007, 114, 129-134.
• 26.Mitchell A. L., Pearce S. H.: Autoimmune Addison disease: pathophysiology and genetic complexity. Nat. Rev. Endocrinol. 2012, 8, 306-316.
• 27.Nebert D. W., Russell D. W.: Clinical importance of the cytochromes P450. Lancet 2002, 360, 1155-6112.
• 28.Notredame C., Higgins D. G., Heringa J.: T-Coffee: A novel method for fast and accurate multiple sequence alignment. J. Mol. Biol. 2000, 302, 205-217.
• 29.Novartis Animal Health.: Addison's Disease: Uncommon or Underdiagnosed? J. Vet. Med. 2003, 50, supplement.
• 30.Nylander J.: MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University 2004.
• 31.Pang S., Yang X., Wang M., Tissot R., Nino M., Manaligod J., Bullock L. P., Mason J. I.: Inherited congenital adrenal hyperplasia in the rabbit: absent cholesterol side-chain cleavage cytochrome P450 gene expression. Endocrinology 1992, 31, 181-186.
• 32.Parker H. G., Kim L. V., Sutter N. B., Carlson S., Lorentzen T. D., Malek T. B., Johnson G. S., DeFrance H. B., Ostrander E. A., Kruglyak L.: Genetic structure of the purebred domestic dog. Science 2004, 304, 1160-1164.
• 33.Rajput N., Gautam V., Sahni Y. P., Jain S.: Cytochrome P450: A Novel Approach In Cancer Therapy. J. Vet. Pharmacol. Tox. 2009, Vol. 8, Issue 1-2/1-8.
• 34.Rogoff D., Gomez-Sanchez C. E., Foecking M. F., Wortsman J., Slominski A.: Steroidogenesis in the human skin: 21-hydroxylation in cultured keratinocytes. J. Steroid. Biochem. Mol. Biol. 2001, 78, 77-81.
• 35.Ronquist F., Huelsenbeck J. P.: MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 2003, 19, 1572-1574.
• 36.Schaer M.: Acute Adrenocortical Insufficiency. Proc. of 30th World Congress of the World Small Animal Veterinary Association 2005.
• 37.Schmeitzel L. P., Lothrop C. D., Rosenkrantz W. S.: Congenital adrenal hyperplasia-like syndrome. Kirk’s Current Veterinary Therapy XII. Philadelphia: Saunders W. B. 1995, 600-604.
• 38.Speiser P. W., Dupont B., Rubinstein P., Piazza A., Kastelan A., New M. I.: High frequency of nonclassical steroid 21-hydroxylase deficiency. Am. J. Hum. Genet. 1985, 37, 650-667.
• 39.Stafford D.: The Great Mimic: Canine Addison's Disease. Vet. Tech. 1999, 20, 490-496.
• 40.Takada K., Kitamura H., Takiguchi M., Saito M., Hashimoto A.: Cloning of canine 21-hydroxylase gene and its polymorphic analysis as a candidate gene for congenital adrenal hyperplasia-like syndrome in Pomeranians. Res. Vet. Sci. 2002, 73, 159-163.
• 41.Tsuda K., Kikkawa Y., Yonekawa H., Tanabe Y.: Extensive interbreeding occurred among multiple matriarchal ancestors during the domestication of dogs: evidence from inter- and intraspecies polymorphisms in the D-loop region of mitochondrial DNA between dogs and wolves. Genes Genet. Syst. 1997, 72, 229-238.
• 42.Vila C., Amorim I. R., Leonard J. A., Posada D., Castroviejo J., Petrucci-Fonseca F., Crandall K. A., Ellegren H., Wayne R. K.: Mitochondrial DNA phylogeography and population history of the grey wolf canis lupus. Mol. Ecol. 1999, 8, 2089-2103.
• 43.Wayne R. K.: Molecular evolution of the dog family. Trends. Genet. 1993, 9, 218-224.
• 44.White P. C., New M. I., Dupont B. O.: Structure of human steroid 21-hydroxylase genes. Proc. Natl. Acad. Sci. USA 1986, 83, 5111-5115.
• 45.Wiebolt J., Koeleman B. P., van Haeften T. W.: Endocrine autoimmune disease: genetics become complex. Eur. J. Clin. Invest. 2010, 40, 1144-1155.
• 46.Yu L., Romero D. G., Gomez-Sanchez C. E., Gomez-Sanchez E. P.: Steroidogenic enzyme gene expression in the human brain. Mol. Cell. Endocrinol. 2002, 190, 9-17.
• 47.Zhou M. Y., Vila M. C., Gomez-Sanchez E. P., Gomez-Sanchez C. E.: Cloning of two alternatively spliced 21-hydroxylase cDNAs from rat adrenal. J. Steroid. Biochem. Mol. Biol. 1997, 62, 277-286.
• 48.Ziaja J., Cholewa K., Mazurek U., Cierpka L.: Molekularne podstawy syntezy aldosteronu i kortyzolu w prawidłowych nadnerczach i w gruczolakach kory nadnerczy. Endokrynol. Pol. 2008, t. 59, nr 4.

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