Transmission, ELISA and SDS-PAGE results of some Maize streak virus isolates from different parts of Nigeria

• Autorzy: Oluwafemi S , Thottappilly G. , Alegbejo M.D.

Warianty tytułu

PL

Przenoszenie sie oraz wyniki testow ELISA i SDS-PAGE wybranych izolatow Maize streak virus pochodzacych z roznych regionow Nigerii

• Języki publikacji: EN

Abstrakty

EN

Field surveys were undertaken in 1997-1999 across five ecological zones in Nigeria to collect isolates of Maize streak virus (MSV), genus Mastrevirus. Apart from maize (Zea mays L.), 15 other grass species were found with MSV symptoms in Nigeria. These hosts showed two types of symptoms viz: mild (with or without mottle) or severe (typical symptoms in maize). When Cicadulina storeyi China was used to attempt transmission of these isolates of MSV to seedlings a susceptible maize hybrid CML 254 X CML 247, six isolates were not transmissible to maize. Seven isolates that were transmissible to maize produced mild symptoms. The viral agents causing typical or severe streak symptoms in Axonopus compressus (Sw.) P. Beauv., Brachiaria distichophylla (Tm.) Stapf, Dactyloctenium aegyptium (Linn.) P. Beauv. and Setaria barbata (Lam.) Kunth produced symptoms that were typical of MSV in farmers fields, when transmitted to maize. Out of 33 plant species that seedlings were challenged with MSV, only eight proved susceptible. Four of them showed mild symptoms while the other four showed severe symptoms of MSV. Only three isolates collected during the surveys did not react with a MSV polyclonal antiserum produced in mice in Double Antibody Sandwich-Enzyme-Linked Immunosorbent Assay (DAS-ELISA). These isolates were found in Andropogon gayanus Kunth (from Ka- duna), Thelepogon elegans Roth ex Toem & Schult (from Kadawa) and Rottboellia cochinchinensis (Lour.) Clayton (from Jos) exhibited mild streak/mottle symptoms. Specific monoclonal antibodies, raised against MSV, reacted with 12 out of 25 samples tested. The DAS-ELISA data also showed significant variation in concentration of the virus in the different plant hosts. The relationship dendogram through SDS-PAGE among eight purified virus isolates show 55-90% variation. At 0.55 coefficient of similarity, the dendogram divided the samples into two groups while at 0.9 coefficient of similarity, the 8 isolates were identified as distinct genetic entities.

PL

W latach 1997-1999 podjęto badania w pięciu strefach ekologicznych Nigerii w celu zebrania izolatów Maize streak virus z rodzaju Mastervirus. Oprócz kukurydzy (Zea mays L.) stwierdzono 15 innych gatunków traw z symptomami infekcji MSV. Badane rośliny żywicielskie miały dwa rodzaje objawów, mianowicie łagodne (z plamami lub bez) albo ostre (typowe objawy, jak w przypadku kukurydzy). Kiedy w próbie przeniesienia izolatów MSV na sadzonki podatnej hybrydy kukurydzy CML 254 X CML 247 użyto Cicadulina storeyi China, sześć z nich nie przeniosło się. Siedem izolatów, które się przenosiły spowodowało wystąpienie łagodnych objawów. Czynniki wirusowe powodujące typowe lub ostre objawy na Axonopus compressus (Sw.) P. Beauv., Brachiaria distichophylla (Tm.) Stapf, Dadyloctenium aegyptinum (Linn.) P. Beauv. i Setaria barbata (Lam.) Kunth spowodowały wystąpienie objawów typowych dla infekcji MSV na polach farmerów kukurydzy, na które zawleczono wirusa. Spośród 33 gatunków roślin, których sadzonki infekowano MSV, tylko osiem było podatnych. Cztery z nich miały łagodne objawy, natomiast cztery pozostałe ostre. Jedynie trzy uzyskane izolaty nie reagowały podczas testu DAS-ELISA (Double Antibody Sandwich - Enzyme Linked Immunosorbent Assay) z poliklonalnym antyserum wyhodowanym na myszach. Izolaty te zebrano z Andropogon gayanus Kunth (z Kaduny), Thelepogon elegans Roth ex Toem & Schult (z Kadawy) i Rottboellia cochinchinensis (Lour.) Clayton (z Jos); rośliny wykazywały łagodne objawy (pasemka/plamki). Zastosowane specyficzne przeciwciała monoklonalne skierowane przeciwko MSV reagowały z 12 próbkami z 25 badanych. Dane DAS-ELISA wykazały również znaczne zróżnicowanie stężeń wirusa w różnych roślinach żywicielskich. Drzewo pokrewieństwa wynikające z SDS-PAGE pomiędzy ośmioma oczyszczonymi izolatami wirusa wykazało 55-90% zróżnicowania. Przy współczynniku prawdopodobieństwa 0,55 dendrogram dzieli próbki na dwie grupy, natomiast przy współczynniku 0,9 osiem powyższych izolatów stanowiło odrębne jednostki genetyczne.

Słowa kluczowe

EN

plant disease; Mastrevirus; isolate; ELISA test; sodium dodecyl sulphate-polyacrylamide gel electrophoresis; transmission; Maize streak virus; Nigeria

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2007
• Tom: 47
• Numer: 2
• Opis fizyczny: p.197-212,fig.,ref.

Twórcy

autor

Oluwafemi S

• International Institute of Tropical Agriculture, Oyo Road, P.M.B.5320, Ibadan, Nigeria

autor

Thottappilly G.

autor

Alegbejo M.D.

Bibliografia

• Akobundu I.O., Agyakwa C.W. 1987. A Handbook of West African Weeds. IITA, Ibadan, 521 pp.
• Asanzi M.C., Bosque-Perez N.A., Buddenhagen I.W., Gordon D.T., Nault L.R., 1994. Interactions among maize streak virus disease, leafhopper vector populations and maize cultivars in forest and savanna zones of Nigeria. Plant Pathol. 43: 145-157.
• Bock K.R. 1974. Maize streak virus. Comm. Mycol. Inst. Assoc. Appl. Biol. 133, p. 4.
• Bock K.R., Guthrie E.J., Woods R.D. 1974. Purification of maize streak virus and its relationship to viruses associated with streak diseases of sugarcane and Panicum maximum. Ann Appl. Biol. 77: 289-296.
• Bosque-Perez N.A., Mareck. J.H. 1990. Distribution and species composition of lepidopterous maize borers in southern Nigeria. Bull. Ent. Res. 80:363-368.
• Briddon R.W., Lunness P., Chamberlin L.C.L., Pinner M.S., Brundish H., Markham P.G. 1992. The nucleotide sequence of an infectious insect-transmissible clone of the geminivirus Panicum streak virus. J. Gen. Virol. 73:1041-1047.
• Briddon R.W., Markham P.G. 1995. Use of PCR in the detection and characterization of geminiviruses. Bull. OEPP/EPPO Bull. 25: 315-320.
• Clark M.F., Adams A.N. 1977. Caharacteristics of the microplate method of enzyme-linked immunosorbent assay for detecting of plant viruses. J. Gen. Virol. 34: 475-483.
• Dekker, E.L., Pinner M.S., Markham P.G., van Regenmotel M.H.V. 1998. Characterisation of maize streak virus isolates from different plant species by polyclonal and monoclonal antibodies. J. Gen. Virol. 69: 983-990.
• D'Urzo M.P., Pedalino M., Grillo S., Rao R., Tucci M. 1990. Variability in major seed proteins in different Vigna species, p. 90-100. In: "Cowpea Genetic Resources" (N.Q. Ng, L.M. Monti, eds.). IITA, Ibadan, Nigeria.
• Efron T., Kim S.K., Fajemisin J.M., Mareck J.H., Tang C.Y., Dąbrowski Z.T., Rossel H.W., Thottapplly G. 1989. Breeding for resistance to maize streak virus: a multidisciplinary team approach. Plant Breed. 103:1-36.
• Fajemisin J.M. 1986. Maize diseases in Africa and their role in the varietal improvement process p. 237-250. In: "To feed ourselves. Proc. First East, Central and South African Regional Maize-Workshop" (B. Gelaw, ed.). CIMMYT, Mexico, D.F.
• Fajemisin J.M., Shoyinka S.A. 1976. Maize streak and other maize virus diseases in West Africa, p. 52-61. In: "Proc. International Maize Virus Disease Colloquium and Workshop". Ohio, 16-19 August, 1976. Agric. Res. Dev. Centre Wooster, USA.
• Fajemisin J.M., Cook G.E., F. Okusanya, Shoyinka S.A. 1976. Maize streak virus epiphytotic in Nigeria. PI. Dis. Reptr. 60: 443-447.
• Fennah R.G. 1959. A new species of Cicadulina (Homoptera: Cicadellidae) from East Africa. Ann. And Mag. Nat. History (series 13) 2: 757-758.
• Fuller C. 1901. Mealie variegation. First Rep. Gov. Entomol. Natal, 1899-1901:17-19.
• IITA 1984. International Institute of Tropical Agriculture. Annual Report for 1983. Ibadan, Nigeria: 42-43.
• Laemmli U.K. 1970. Cleavage of structural proteins during the assembly of the head of Bacteriophage T4. Nature 227: 680-685.
• Larsen R.C., Duffus J.E. 1983. A simplified procedure for the purification of curly top virus and the isolation of its monomers and dimer particles. Phytopathology 73: 114-118.
• McClean A.P.D. 1947. Some forms of streak virus occurring in maize, sugarcane and wild grasses. Union. South African Dept. Agric. Sci. Bull. 265: 1-39.
• Mesfin T. Bosque-Perez N.A., Buddenhagen I.W., Thottappilly G., Olojede S.0.1992. Studies of maize streak virus isolates from grass and cereal hosts in Nigeria. Plant Dis. 76: 789-795.
• Okoruwa A.E. 1995. Utilization and Processing of Maize. IITA Research Guide 35, 29 pp.
• Olojede S.0.1987. Transmission and population studies on maize streak virus vectors: Cicadulina spp. (Homoptera: Cicadelidae). M.Sc. Thesis. Ahmadu Bello University, Zaria, 78 pp.
• Peterschmitt M., Reynaud B., Sommermeyer G., Baudin P. 1991. Characterization of maize streak virus isolates using monoclonal and polyclonal antibodies and by transmission to a few hosts. Plant Dis. 75: 27-32.
• Pinner M.S., Markham P.G. 1990. Serotyping and strain identification of maize streak virus isolates. J. Gen. Virol. 71:1635-1640.
• Pinner M.S., Markham P.G., Markham R.H., Dekker E.L. 1988. Characterization of maize streak virus: description of strains; symptoms. Plant Pathol. 37: 74-87.
• Reynaud B., Peterschmitt M. 1992. A study of the mode of transmission of maize streak virus by Cicadulina mbila using an enzyme - linked immunosorbent assay. Ann. Appl. Biol. 121: 85-94.
• Rose D.J.W. 1962. Insect vectors of maize streak. Zool. Soc. South Africa News Bull. 3, p. 11.
• Rose D.J.W. 1978. Epidemology of maize streak disease. Ann. Rev. Ent. 23: 259-282.
• Rossell H.W., Thottappilly G. 1985. Virus Diseases of Important Food Crops in Tropical Africa. IITA, Ibadan, Nigeria, 61 pp.
• Sneath P.H.., Sokal R.R. 1973. Numerical Taxonomy: The Principles and Practice of Numerical Classification. W.H. Freeman & Co. San Franscisco, USA, 573 pp.
• Soto P.E., Buddenhagen I.W., Asnani V.L. 1982. Development of streak virus-resistant maize populations through improved challenge and selection methods. Ann. Appl. Biol. 100: 539-546.
• Storey H.H. 1925. The transmission of streak virus of maize by the leafhopper Balclutha mbila Naude. Ann. Appl. Biol.,12:422M39.
• Storey H.H. 1928. Transmission studies of maize streak disease. Ann. Appl. Biol. 15:429-436.
• Storey H.H., McClean A.P.D. 1930. The transmission of streak disease between maize, sugarcane and wild grasses. Ann. Appl. Biol. 17: 691-719.
• Thottappilly G., Bosque-Perez N.A., Rossel H.W. 1993. Viruses and virus diseases of maize in Tropical Africa. Plant Path. 42: 494-509.