Application of anti-stress products in aquaculture: influence of propiscin on the effectiveness of an anti-Yersinia ruckeri vaccine in rainbow trout Oncorhynchus mykiss [Wall.]

• Autorzy: Siwicki A K , Morand M , Kazun K , Keck N , Glabski E , Malaczewska J
• Języki publikacji: EN

Abstrakty

EN

Inducing protection against bacterial diseases in fish culture through the application of specific vaccines is one of the most important prevention methods. However, some immunization techniques actually used under hatchery conditions are not as effective as they should be. Therefore, current research is focusing on how to improve the potency and efficacy of the antigens and how to optimally activate the immune response. In our preliminary study, we determined the influence of the anaesthetic Propiscin (IFI, Poland) on the effectiveness of a vaccine applied by intraperitoneal injection or immersion to rainbow trout (Oncorhynchus mykiss). The fish were vaccinated via injection or immersion with an anti-Yersinia ruckeri vaccine (Sanofi, France) under Propiscin-induced anaesthesia or without it. On day 21 after vaccination, all the fish groups were challenged with live Y. ruckeri bacteria (0.2 ml of bacteria at 1 x 10⁶ ml⁻¹ ). Control groups (only anaesthetized and injected PBS or immersed) were infected but not immunized by vaccine. The blood and pronephros were separated before vaccination (10 fish), 21 days after vaccination (10 fish) and 21 days after the challenge (10 fish). ELISPOT assays for the quantification of total immunoglobulin secreting cell (ISC) and specific antibody secreting cell levels (ASC) were used. The total Ig levels in the serum were also measured by spectrophotometric assay and the titers of anti-Y. ruckeri antibody were measured by the micro-agglutination method. Mortalities were collected and tabulated and the presence of pathogen was confirmed by isolation from fish kidneys. The results of this study showed that Propiscin applied at vaccination time increased the nonspecific and specific immune response and protective effectiveness of vaccine after the challenge with pathogenic bacteria. The highest level of total ISC and specific ASC in fish vaccinated by immersion and injections under anaesthesia were observed. The highest total Ig levels and titers of specific antibody in fish vaccinated under anaesthesia were also observed. Cumulative mortality rates were lowest in the fish vaccinated by injection (5%) and immersion (10%) under anaesthesia as compared with fish vaccinated by injection (20%) and immersion (35%) without anaesthesia. Cumulative mortality in the control, non-vaccinated group of fish was 80%.

PL

Stosowanie swoistych szczepionek jest najbardziej efektywną metodą ochrony zdrowia ryb. Jednakże przy użyciu aktualnych technik immunizacyjnych uzyskiwane efekty szczepień nie są w pełni zadowalające. Prowadzone są badania nad zwiększeniem efektywności szczepień przez stosowanie środków antystresowych, eliminujących negatywny wpływ stresu polietiologicznego na organizm ryb. Celem prezentowanych badań było określenie wpływu znieczulenia ogólnego preparatem Propiscin (IRS) na efektywność szczepionki przeciwko Yersinia ruckeri podawanej w iniekcji lub immersji pstrągom tęczowym (Oncorhynchus mykiss). Wyniki porównywano z uzyskanymi bez zastosowania znieczulenia ogólnego. W celu sprawdzenia efektywności szczepienia 21 dnia po wakcynacji ryby poddano zakażeniu żywymi bakteriami Y. ruckeri. Bezpośrednio przed i 21 dni po zakażeniu od części ryb pobierano krew i nerkę głowową. Określano ogólną ilość komórek produkujących przeciwciała (ISC), poziom komórek produkujących swoiste przeciwciała (ASC), poziom immunoglobulin (Ig) w surowicy oraz miano swoistych przeciwciał anti-Y. ruckeri. Po zakażeniu eksperymentalnym kontrolowano stan kondycyjny oraz rejestrowano liczbę śnięć. Uzyskane wyniki przedstawiono na odpowiednich wykresach (rys. 1, 2,3,4,5). Wyniki badań jednoznacznie wykazały, że u ryb poddanych szczepieniu zarówno w iniekcji jak i w immersji w pełnym znieczuleniu ogólnym stwierdzono statystycznie istotnie wyższą odpowiedź immunologiczną określaną poziomem ISC, ASC, Ig oraz mianem swoistych przeciwciał. Również obserwowano znacznie niższy procent śmiertelności po zakażeniu eksperymentalnym u ryb poddanych immunizacji w znieczuleniu ogólnym.

Słowa kluczowe

EN

Yersinia ruckeri; Oncorhynchus mykiss; vaccine; application; effectiveness; anti-stress product; rainbow trout; aquaculture; immune response

• Wydawca: -
• Czasopismo: Archives of Polish Fisheries
• Rocznik: 2002
• Tom: 10
• Numer: 2
• Opis fizyczny: p.143-152,fig.,ref.

Twórcy

autor

Siwicki A K

• The Stanislaw Sakowicz Inland Fisheries Institute in Olsztyn, Olsztyn, Poland
• Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Poland

autor

Morand M

• Laboratoire Departemental d’ Analyses, Conseil General du Jura, France

autor

Kazun K

• The Stanislaw Sakowicz Inland Fisheries Institute in Olsztyn, Olsztyn, Poland

autor

Keck N

• Laboratoire Departemental d’ Analyses, Conseil General du Jura, France

autor

Glabski E

• The Stanislaw Sakowicz Inland Fisheries Institute in Olsztyn, Olsztyn, Poland

autor

Malaczewska J

• Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Poland

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