Evaluation of an ELISA for the diagnosis of sarcocystosis in water buffaloes

• Autorzy: Ghorbanpoor M , Hamidinejat H. , Nabavi L. , Khadjeh G.H. , Jalali M.R.
• Języki publikacji: EN

Abstrakty

EN

To investigate the occurrence of sarcocystosis in water buffalo (Bubalus bubalis) in Ahvaz, the Khuzestan province, Iran, and to evaluate an ELISA for the diagnosis of sarcocystosis, serum and oesophagus samples were collected from the 300 water buffaloes, aged 0.5-7 years, and then slaughtered at the Ahvaz abattoir. The oesophagus samples were examined for sarcocysts under microscopic examination, using the digestion method. One hundred and seventy-one (57%) animals were found to be positive for Sarcocystis bradyzoites. One hundred and sixty-three (54.3%) serum samples were positive for sarcocystis antibodies in the ELISA. The prevalence of sarcocystosis was statistically age related, with significantly higher rates in adult buffaloes than young animals (P<0.05). The prevalence did not differ significantly in relation to the gender (P>0.05). The Mc Nemar test revealed a high correlation (94%) between the digestion method and ELISA. The ELISA, with the use of antigens from S. fusiformis bradyzoites, as presented in this study, can be adapted to detect antibodies to Sarcocystis sp., with an acceptable specificity and sensitivity.

Słowa kluczowe

EN

Sarcocystis; diagnosis; ELISA test; zoonotic disease; animal disease; Iran; sarcocystosis prevalence; water buffalo; sarcocystosis; domestic animal

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2007
• Tom: 51
• Numer: 2
• Opis fizyczny: p.229-231,ref.

Twórcy

autor

Ghorbanpoor M

• Shahid Chamran University, 61355-145 Ahvaz, Iran

autor

Hamidinejat H.

autor

Nabavi L.

autor

Khadjeh G.H.

autor

Jalali M.R.

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