Signal transduction in confluent C3H 10T1-2 cells. The role of focal adhesion kinase

• Autorzy: Miloszewska J. , Trembacz H. , Janik P.
• Języki publikacji: EN

Abstrakty

EN

The activities of extracellular signal-regulated kinases (ERK1/ERK2) is required for proliferation of several types of cells. The performed analysis showed stimulation of ERK's by fetal calf serum (FCS) or fibronectin in the C3H 10T1/2 cell cultures at logarith­mic phase of growth. The ERKs activity was not stimulated in confluent cells. This could not be accounted for a partial down regulation of ERK since its level was stable in both types of cells regardless of their density and kind of stimulation. Searching for ERK up­stream elements we studied the integrin receptor gene transcript by RT-PCR and focal ad­hesion kinase (FAK) by Western blotting and phosphorylation assays. It was found that FCS and fibronectin stimulated phosphorylating activity of FAK in the cells at the logarithmic phase of growth, but were inefficient in the confluent cells. RT-PCR showed the presence of α5 and β1 integrin transcripts, and p125FAK was at the same level regardless of the type of stimulation. These data indicate that the ability of FAK to be activated plays an important role in ERK regulation and, in consequence in proliferation and growth inhibition during confluence.

Słowa kluczowe

EN

extracellular signal-regulated kinase; proliferation; cell; focal adhesion kinase; signal transduction; contact inhibition; integrin; fibronectin; growth factor

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 1
• Opis fizyczny: p.175-181,fig.

Twórcy

autor

Miloszewska J.

• The Maria Curie-Sklodowska Memorial Cancer Center and Institute of Oncology, W.K.Roentgena 5, 02-781 Warsaw, Poland

autor

Trembacz H.

autor

Janik P.

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