Wplyw zwiekszonej zawartosci ozonu w powietrzu na wybrane wskazniki biochemiczne, hematologiczne i immunologiczne u szczurow w warunkach podawania w paszy zroznicowanych dawek witaminy E i C. IV. Wplyw zwiekszonej zawa...

• Autorzy: Zielinski H , Jakubowski K.
• Języki publikacji: PL

Abstrakty

PL

Badania przeprowadzono na 180 szczurach szczepu Wistar/Hannower w trzech oddziel­nych doświadczeniach E, E+C i C. W każdym z doświadczeń zwierzęta dzielono na sześć grup w zależności od sposobu ekspozycji i dodatku witaminy E i С w paszy standardowej. Zwierzęta z grup kontrolnych oraz grup II żywiono paszą standardową dla gryzoni „Murigran", natomiast z grup III-VI, w zależności od doświadczenia, paszą wzbogaconą witaminą E, E+C lub C. Zwierzęta z grup II - VI eksponowano na 1.0 ± 0.4 mg O3/ m3 powietrza przez okres 5 tygodni, 8 godz. dziennie. Po zakończeniu doświadczeń w osoczu krwi oznaczono poziom fluorescencji frakcji lipidowej i wodnej produktów peroksydacji lipidów, poziom dialdehydu malonowego i witaminy С oraz określono zawartość białka całkowitego. Wyniki poddano analizie statystycznej. Wykazano celowość żywienia szczurów paszą wzbogacaną witaminą E i С w warunkach narażenia ich na wielokrotnie powtarzane ekspozycje na powietrze o zwiększonej koncentracji ozonu.

EN

The studies, divided into three experiments designed as E, E+C and С were carried out with one hundred and eighty male Wistar/Hannover rats. In each experiment the animals were divided into six groups depending on the mode of exposition and additional amounts of vitamin E and С in standard food. The animals from control groups and groups II were fed with a commercial folder „Murigran". The rats from groups III—VI were fed with a commercial fodder supplemented with multiple amounts of vitamin E, E+C and C. The animals from groups II-VI were exposed to ozone ( 1.0 ± 0.4 mg/m3 ) with duration of 5 weeks, eight hours a day. After completing the experiments the blood from the rats was collected. The level of fluorescence of lipid and water soluble lipid peroxidation products, content of malondialdehyde, vitamin С and total proteins in blood serum were determined by the spectrophotometric methods. Data were analyzed according to statistics. The studies showed disadvantagous influence of ozone on rats' organisms and it was visible in increase of lipid peroxidation processes. The studies also showed that moderate increases in vitamin E and С intake to supplement the average daily diet for rats might be beneficial when animals are exposed to the increased concentration of ozone in the air.

Słowa kluczowe

PL

zwierzeta doswiadczalne; szczury; zywienie zwierzat; pasze; wskazniki biochemiczne; wskazniki hematologiczne; wskazniki immunologiczne; osocze; zawartosc witamin; witamina C; witamina E; zanieczyszczenia powietrza; ozon

EN

experimental animal; rat; animal feeding; feed; biochemical index; hematological index; immunological index; plasma; vitamin content; vitamin C; vitamin E; air contaminant; ozone

• Wydawca: -
• Czasopismo: Acta Academiae Agriculturae ac Technicae Olstenensis. Veterinaria
• Rocznik: 1997
• Tom: 25
• Opis fizyczny: s.65-75,tab.,bibliogr.

Twórcy

autor

Zielinski H

• Instytut Rozrodu Zwierzat i Badan Zywnosci PAN, Olsztyn

autor

Jakubowski K.

Bibliografia

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