Dojrzewanie in vitro oocytow kota domowego

• Autorzy: Grabiec A , Faundez R. , Max A. , Tischner M.

Warianty tytułu

EN

In vitro maturation of domestic cat oocytes

• Języki publikacji: PL

Abstrakty

EN

The aim of this study was to compare the efficiency of in vitro maturation in relationship to pathomorphology of uterus and oocytes quality. Morphological evaluation of the uterus helped to classify the queens into one of three groups: those with normal uteri, those with transformed uteri and pregnant queens. Cumulus-oocyte complexes were separated at recovery into three types according to pigmetation, uniformity and smoothness of ooplasm, compactness and number of layers of cumulus cells, as well as integrity of zona pellucida. Oocytes were maturated for 36 hours at 38°C in M199 medium containing cysteine and 17b oestradiol. After IVM, the meiotic status of oocytes was determined and the percentage of matured oocytes was calculated in relationship to COCs quality and uterus condition. In the group with normal uterus, oocytes of class A maturated (63.93%) better than oocytes class B and C, although the differences in maturation of oocytes class A and B were not statistically significant. The worst results of IVM were for oocytes class C. In the group of transformed uterus, 55.17% of oocytes class A maturated, while in only 7.84% of oocytes class C metaphase II had been observed. Significant differences were established in availability to IVM between oocytes class A and C as well as class B and C. In the group with pregnant uteri, the highest percentage of matured oocytes was in class A (46.27%), though there are no statistically significant differences in IVM between oocytes class A and B (28.8%), and the oocytes class C maturated in only 1.82%. Only oocytes of class A and B should be qualified for IVM procedure. Pregnant queens as well as queens with transformed uteri can also serve as oocyte donors for in vitro maturation.

Słowa kluczowe

PL

dojrzewanie in vitro; koty; oocyty; dojrzewanie oocytow; rozrod zwierzat

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2007
• Tom: 63
• Numer: 03
• Opis fizyczny: s.318-321,fot.,tab.,bibliogr.

Twórcy

autor

Grabiec A

• Szkola Glowna Gospodarstwa Wiejskiego, ul.Nowoursynowska 159C, 02-776 Warszawa

autor

Faundez R.

autor

Max A.

autor

Tischner M.

Bibliografia

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