Identification of RAPD markers and their use for molecular mapping in pea [Pisum sativum L.]

• Autorzy: Cheghamirza K , Koveza O. , Konovalov F. , Gostimsky S.
• Języki publikacji: EN

Abstrakty

EN

The RAPD method (Random Amplified Polymorphic DNA) was used for identifying and mapping new molecular markers in pea. RAPD analysis of various cultivars and lines of pea was carried out using 10-mer random primers. The presence of multiple polymorphism between cultivars and lines was revealed; at least one fragment for any given primer was present in the DNA of one form of pea and absent in the DNA of another line or cultivar. To detect molecular markers linked to the genes of chi-15, xa-18 and also to the 12 morphological markers of the L-1238 line, the F2 populations (Chi-15 x L-1238), (Vio x L-1238), (Xa-18 x L-1238), (L-111 x Chi-15) and (L-84 x Xa-18) were studied via bulked segregant analysis. DNA molecular analysis of F1 hybrids revealed the presence of parental polymorphic fragments in all of the populations. The study of the F2 plants showed that the obtained fragments are inherited as Mendelian factors. 13 RAPD-markers linked to genes of A/a (flower color), I/i (seed color), Gp/gp (pod color), R/r (seed form), S/s (seeds linkage), and also to genes of Chi-15/chi-15 (leaf color) and Xa-18/xa-18 (leaf color) were discovered. The study of individual plant DNA from the F2 populations allowed us to determine the genetic distances between genes and the RAPD markers linked to them.

Słowa kluczowe

EN

random amplified polymorphic DNA; F2 plant; plant genetics; genetic mapping; pea; molecular marker; higher plant; Pisum sativum; mapping; plant breeding

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2002
• Tom: 07
• Numer: 2B
• Opis fizyczny: p.649-655,fig.

Twórcy

autor

Cheghamirza K

• Moscow State University, Vorobjevy Gory, Moscow, 119899, Russia

autor

Koveza O.

autor

Konovalov F.

autor

Gostimsky S.

Bibliografia

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