PCR in the diagnosis of bovine leukaemia virus infection

• Autorzy: Kubis P , Rulka J. , Kur J. , Dabrowski S.
• Języki publikacji: EN

Abstrakty

EN

Whole blood samples from 73 animals from a dairy herd infected with BLV were used for detection of proviral DNA by means of PCR technique. At the beginning 24 cows were examined with primer set ZM1/ZM2. Seven out of 13 ELISA-positive animals –were PCR-positive too and 3 were PCR-positive among 11 serologically negative. Due to unsatisfactory results the rest of the samples were tested with a second primer set ZM2/ZM3. In this study 20 cows out of 25 ELISA-positive were PCR-positive and 8 out of 24 seronegative cows were PCR-positive. The findings of seronegative PCR-positive and seropositive PCR-negative cattle indicate that further work is needed. We plan to use the nested PCR because this technique seems to be more specific and sensitive.

Słowa kluczowe

EN

cow; B lymphocyte; infection; virus; cattle; diagnosis; ELISA test; proviral DNA; dairy herd; bovine leukemia virus; polymerase chain reaction; blood sample

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 1996
• Tom: 40
• Numer: 2
• Opis fizyczny: p.85-89,fig.

Twórcy

autor

Kubis P

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Rulka J.

autor

Kur J.

autor

Dabrowski S.

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