Partial characterization of human choriocarcinoma cell line JAR cells in regard to oxidative stress

• Autorzy: Hallmann A. , Klimek J. , Masaoka M. , Kaminski M. , Kedzior J. , Majczak A. , Niemczyk E. , Wozniak M. , Trzonkowski P. , Wakabayashi T.
• Języki publikacji: EN

Abstrakty

EN

Characterization of free radical-induced cell injury processes of placenta cells is of vital importance for clinical medicine for the maintenance of intrauterine fetal life. The present study has analyzed cell injury processes in cells of the choriocarcinoma cell line JAR treated with menadione, an anticancer drug, and H2O2 in comparison to osteosarcoma 143B cells using electron microscopic and flow cytometric tech­niques. Flow cytometry on JAR cells exposed to 100 ^M menadione and dou­ble-stained with Annexin V and propidium iodide (PI) detected apoptotic cells reach­ing the maximum after 4 h of incubation with a rapid decrease thereafter. Viable cells became decreased to 46% of the control after 2 h of incubation, reaching 5% af­ter 4 h. Cells stainable with both Annexin V and PI began to increase distinctly after 2 h of incubation, reaching 55% after 4 h. Electron microscopy showed that cells stainable with both dyes specified above had condensed nuclei and swollen cyto­plasm, suggesting that they were undergoing a switch of the cell death mode from apoptosis to necrosis. On the other hand, 90% of 143B cells remained intact after 4 h of menadione treatment although the intracellular levels of superoxide were always higher than those of JAR cells treated with the drug. In contrast, JAR cells were more resistant than 143B cells to H2O2-induced cytotoxicity. These results may suggest that cytotoxicity of menadione cannot be explained simply by oxygen free radicals generated from the drug. The resistance of JAR cells to oxygen free radical-induced cytotoxicity may be advantageous for intrauterine fetal life.

Słowa kluczowe

EN

cell line; man; menadione; JAR cell; oxidative stress; flow cytometry; placenta; choriocarcinoma

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2004
• Tom: 51
• Numer: 4
• Opis fizyczny: p.1023-1038,fig.,ref.

Twórcy

autor

Hallmann A.

• Medical University of Gdansk, Debinki 1, 80-210 Gdansk, Poland

autor

Klimek J.

autor

Masaoka M.

autor

Kaminski M.

autor

Kedzior J.

autor

Majczak A.

autor

Niemczyk E.

autor

Wozniak M.

autor

Trzonkowski P.

autor

Wakabayashi T.

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