Wplyw stresu transportowego na stopien wrazliwosci leukocytow na dzialanie leukotoksyny Mannheimia haemolytica

• Autorzy: Urban-Chmiel R , Puchalski A. , Wernicki A.

Warianty tytułu

EN

Influence of transport stress on susceptibility of bovine leukocytes on cytotoxic effect of M.haemolytica leukotoxin

• Języki publikacji: PL

Abstrakty

EN

The purpose of the study was estimating the viability and susceptibility effect of leukocytes isolated from cattle before and after the transportation in vitro on M. haemolytica leukotoxin (Lkt) cytotoxicity. 40 Simentaler heifers that were transported by truck a distance of 1700 km for 72 hours were used in the experiment. The material for the study was the blood (40 samples) collected on heparin directly before and after transportation. In relation to leukocytes the examination of susceptibility on cytotoxic effect of Lkt has been carried out with the use of MTT (microtitration assay) and the viability of leukocytes after 1, 2, 3 and 6 hour of incubation. The results obtained in the cell viability test did not show statistically significant differences (P≥0.05) in 1st and 2nd hour of incubation in leukocytes obtained from heifers before and after transportation. After the 1st hour of incubation the percentage of leukocyte viability was on a very high level and showed 87% in both groups of animals. The significantly lower cell viability values in comparison to leukocytes isolated from animals before the transportation was observed in the transported heifers from 3rd to 6th hour of incubation. The analysis of the results obtained by MTT test indicated statistically significant differences in the susceptibility of leukocytes for cytotoxic activity of Lkt. The average values of toxic activity of Lkt in relation to leukocytes isolated before and after transportation was 79% and 92% respectively. The lytic activity of Lkt for 50% of the cell population referred as 1 unit (1U) was observed in Lkt concentration 15 mg/ml (leukocytes before transportation) and 7.5 mg/ml (after the transportation). The increase of susceptibility of leukocytes isolated after transportation on the cytotoxic effect of leukotoxin suggest the significant influence of transporting stress on the increase of respiratory diseases caused by M. haemolytica strains.

Słowa kluczowe

PL

Mannheimia haemolytica; transport; leukocyty; leukotoksyna; bydlo; cytotoksycznosc; stres transportowy

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 09
• Opis fizyczny: s.1045-1047,tab.,rys.,bibliogr.

Twórcy

autor

Urban-Chmiel R

• Akademia Rolnicza, ul.Akademicka 12, 20-033 Lublin

autor

Puchalski A.

autor

Wernicki A.

Bibliografia

• 1. Ackerman M. R., Brogden K. A.: Response of the ruminant respiratory tract to Mannheimia (Pasteurella) haemolytica. Microb. Infect. 2000, 2, 1079-1088.
• 2. Ambagala T. C., Ambagala A. P., Srikumaran S.: The leukotoxin of P. haemolytica bind to beta (2) integrins on bovine leukocytes. Microb. Lett. 1999, 179, 161-167.
• 3. Baluyut C. S., Simonson R. R., Bemrick W. J., Maheswaran S. K.: Interaction of Pasteurella haemolytica with bovine neutrophils: Identyfication and partial characterization of a cytotoxin. Am. J. Vet. Res. 1981, 42, 1920-1926.
• 4. Clarke L. B., Moore D. R., Blalock J. E.: Adrenocorticotropic hormone stimulates a transient calcium uptake in rat lymphocytes. Endocrinol. 1994, 135, 1780-1786.
• 5. Clinkebeard D. K., Upton L. M.: Lysis of bovine plateles by P. haemolytica leukotoxin. Am. J. Vet. Res. 1991, 52, 453-457.
• 6. Clinkenbeard K. D., Clarke C. R., Morton R. J., Panciera R. J., Confer A. W., Mosier D. A.: Role of P. haemolytica leukotoxin in virulence and immunity in shipping fever pneumonia. Comp. Food Anim. Pract. 1992, 14, 1249-1263.
• 7. Cox E., Mast J., MacHugh N., Schwenger B., Goddeeris B.: Expression of β2 integrins on blood leukocyte adhesion deficiency. Vet. Immunol. Immunopathol. 1997, 58, 249, 263.
• 8. Deshpande M. S., Ambagala T. C., Ambagala A. P. N., Kehrli M. E., Srikumaran S.: Bovine CD18 is necessary and sufficient to mediate Mannheimia (Pasteurella) haemolytica leukotoxin - induced cytolysis. Infect. Immun. 2002, 70, 5058-5064.
• 9. Frank G. H.: When Pasteurella haemolytica colonizes the nasal passages of cattle. Vet. Med. 1988, 83, 1060-1064.
• 10. Fukushima T., Ichinose M., Shingai R., Sawada M.: Adrenocorticotropic hormone activates an outward current in cultured mouse peritoneal macrophages. J. Neuroimmunol. 2001, 113, 231-235.
• 11. Gahmberg C., Valmu L., Fagerholm S., Kotovuori P., Ihanus E., Tian L., Morikowa-Pessa T.: Leukocyte integrins and inflammation. Cell. Mol. Life Sci. 1998, 54, 549-555.
• 12.Gresham N. C., Confer A. W., Bush J.: Serum and colostrum antibody to Pasteurella haemolytica species in dairy cattle. Am. J. Vet. Res. 1984, 45, 2227-2234.
• 13. Gibbs H. A., Allen M. J., Wiseman A., Selman J. E.: Pneumonic pasteurellosis in housed, weaned, single suckled calves. Vet. Rec. 1983, 22, 87-92.
• 14. Jeyaseelan S., Kannan M., Hsuan S., Singh A., Walseth T., Maheswaran S.: Pasteurella (Mannheimia) haemolytica leukotoxin-induced cytolysis of bovine leukocytes: role of arachidonic acid and its regulation. Microb. Pathog. 2001, 30, 59-69.
• 15. Knowles T. G.: A review of the road transport of cattle. Vet. Rec. 1995, 144, 197-201.
• 16. Lan H. C., Reddy P. G., Chambers M. A., Walker G., Srivastava K. K., Ferguson J. A.: Effect of stress on interleukin-2 receptor expression by bovine mononuclear leukocytes. Vet. Immun. Immunopath. 1995, 49, 241-249.
• 17. Li J., Clinkenbeard K. D., Ritchey J. W.: Bovine CD18 identified as a species specific receptor for P. haemolytica leukotoxin. Vet. Microb. 1999, 67, 91-97.
• 18. Leite F., Sylte M., Brien S., Schulz R., Peek S., van Reeth K., Czuprynski C.: Effect of experimental infection of cattle with bovine herpesvirus (BHV-1) on the ex vivo interaction of bovine leukocytes with Mannheimia (Pasteurella) haemolytica leukotoxin. Vet. Immun. Immunopath. 2002, 84, 97-110.
• 19. Mackenzie A., Drennan M., Rowan T., Dixon J., Carter S.: Effect of transportation and weaning on humoral immune response of calves. Res. Vet. Sci. 1997, 63, 227-230.
• 20. Meyer D., Coles E., Rich L.: Leukocytic tests and disorders. Vet. Lab. Med. 1992, 47 27-42.
• 21. Palme R., Robia Ch., Messmann S., Hofer J., Möstl E.: Measurement of faecal cortisol metabolites in ruminants: a non-invasive parameter of adrenocortical function. Wien. Tierärztl. Mschr. 1999, 86, 237-241.
• 22. Roth J., Kaeberle M., Hsu W.: Effects of ACTH administration on bovine polymorphonuclear leukocyte function and lymphocyte blastogenesis. Am. J. Vet. Res. 1982, 43, 412-416.
• 23. Shewen P. E., Wilkie B.: Cytotoxin of Pasteurella haemolytica acting on bovine leukocytes. Infect. Immun. 1982, 35, 91-94.
• 24. Santoro M. G.: Heat Shock Factors and the control of the stress response. Biochem. Pharmacol. 2000, 59, 55-63.
• 25. Sun Y., Clinkenbeard K. D.: Serum-free culture of P. haemolytica optimized for leukotoxin production. Am. J. Vet. Res. 1998, 59, 851-855.
• 26. Vega M. V., Maheswaran S. K., Leininger J. R., Ames T. R.: Adaptation of a colorimetric microtitration assay for quantifying Pasteurella haemolytica A1 leukotoxin and antileukotoxin. Am. J. Vet. Res. 1987, 48, 1559-1564.
• 27. Wernicki A., Urban-Chmiel R., Mikucki P., Puchalski A., Kankofer M.: The influence of transport stress on the humoral immunological response of calves induced by Mannheimia haemolytica antigen. Polish J. Vet. Sci. 2003a, 6, 41-45.
• 28. Wernicki A., Urban-Chmiel R., Mikucki P., Puchalski A., Kankofer M.: Wpływ stresu transportowego na wybrane wskaźniki odporności komórkowej u bydła. Medycyna Wet. 2003b, 59, 608-611.
• 29. Yoo H., Rajagopal B., Maheswaran S., Ames T.: Purified P. haemolytica leukotoxin induces expression of inflammatory cytokines from bovine alveolar macrophages. Microb Path. 1995, 18, 237-252.
• 30. Zavy M., Juniewicz P., Phillips W., VonTungeln D.: Effect of initial resistant, weaning and transport stress on baseline and ACTH-stimulated cortisol responses in beef calves of different genotypes. Am. J. Vet. Res. 1992, 53, 555-557.
• 31. Rozporządzenie Ministra Infrastruktury z dnia 6 października 2003 r. w sprawie szczegółowych warunków i sposobu transportu zwierząt (Dz. U. Nr 185, poz. 1809).
• 32. Ustawa Prezydenta RP z dnia 6 czerwca 2002 r. o zmianie ustawy o ochronie zwierząt (Dz. U. Nr 135).