Development of a multiplex PCR [m-PCR] test for rapid identification of genes encoding heat-labile [LTI] and heat-stable [STI and STII] toxins of enterotoxigenic Escherichia coli [ETEC] with internal control of amplification

• Autorzy: Weiner M , Osek J.
• Języki publikacji: EN

Abstrakty

EN

A multiplex PCR system was developed for specific identification of genes encoding heat-labile (LTI) and heat-stable (STI and STII) toxins of enterotoxigenic Escherichia coli (ETEC) strains. In addition, primers specific for the E. coli gene coding for 16S rRNA were used as an internal control of the DNA amplification. The specificity of the method was validated by single PCR tests performed with reference to E. coli strains as well as pig-isolated bacteria and 100% correlation was observed. The developed multiplex PCR allowed rapid and specific identification of enterotoxin-positive E. coli and may be used as a sensitive and specific method for a direct determination of ETEC and to differentiate them from other E. coli isolates.

Słowa kluczowe

EN

gene coding; gene encoding; Escherichia coli; polymerase chain reaction; entero toxin; DNA amplification; identification

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2004
• Tom: 53
• Numer: 1
• Opis fizyczny: p.7-10,fig.,ref.

Twórcy

autor

Weiner M

• National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland

autor

Osek J.

Bibliografia

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