Was reduced pollen viability in Viola tricolor L. the result of heavy metal pollution or rather the tests applied?

• Autorzy: Slomka A , Kawalec P. , Kellner K. , Jedrzejczyk-Korycinska M. , Rostanski A. , Kuta E.
• Języki publikacji: EN

Abstrakty

EN

We used different tests to assess the effect of high soil concentrations of heavy metals on pollen viability in plants from metallicolous (MET) and nonmetallicolous (NONMET) populations. The frequency of viable pollen depended on the test applied: MET plants showed no significant reduction of pollen viability by acetocarmine, Alexander, MTT and X-Gal dye testing, but a drastic reduction of pollen viability in MET flowers (MET 56% vs 72% NONMET) by the FDA test. There was no correlation between pollen viability estimated in histochemical tests and pollen germination in vitro or in vivo. We discuss the terminology used to describe pollen viability as determined by histochemical tests.

Słowa kluczowe

EN

polluted environment; wild pansy; Viola tricolor; pollen viability; viability test; viability loss; heavy metal; heavy metal pollution; pollen germination; metalliferous soil; acetocarmine test; FDA test; MTT test; X-Gal test

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2010
• Tom: 52
• Numer: 1
• Opis fizyczny: p.123-127,fig.,ref.

Twórcy

autor

Slomka A

• Jagiellonian University, Grodzka 52, 31-044 Krakow, Poland

autor

Kawalec P.

autor

Kellner K.

autor

Jedrzejczyk-Korycinska M.

autor

Rostanski A.

autor

Kuta E.

Bibliografia

• BISKUP A, and IZMAIŁOW R. 2004. Endosperm development in seed of Echium vulgare L. (Boraginaceae) from polluted sites.Acta Biologica Cracoviensia Series Botanica 46: 29–44.
• CZAPIK R [ed]. 2002. Embryological and cytological variability of plants in polluted environment. Polish BotanicalStudies, vol. 15. Polish Academy of Sciences, W. SzaferInstitute of Botany, Krakow.
• DAFNI A. 1992. Pollination Ecology: A Practical Approach (practical approach series). Oxford University Press,USA.
• DAFNI A, and FIRMAGE D. 2000. Pollen viability and longevity: practical, ecological and evolutionary implications. PlantSystematics and Evolution 222(1–4): 113–132.
• DECHAMPS C, LEFEBVRE C, NORT N, and MEERTS P. 2007. Reaction norms of life history traits in response to zinc in Thlaspi caerulescens from metalliferous and nonmetalliferoussites. New Phytologist 173(1): 191–198.
• DENG J, LIAO B, YE M, DENG D, LAN CH, and SHU W. 2007. The effect of heavy metal pollution on genetic diversity inzinc/cadmium hyperaccumulator Sedum alfredii populations.Plant and Soil 297(1–2): 83–92.
• ERNST WHO, VERKLEIJ JAC, and SCHAT H. 1992. Metal tolerance in plants. Acta Botanica Neerlandica 41(3): 229–248.
• HOEKSTRA FA, and BRUINSMA J. 1975. Respiration and vitality of binucleate and trinucleate pollen. Physiologia Plantarum34: 221–225.
• IZMAIŁOW R. 2000. Reproduction of Viccia cracca L. in the polluted environment of the Legnica – Głogow copper basin(Poland). Acta Biologica Cracoviensia Series Botanica42(2): 125–133.
• IZMAIŁOW R, and BISKUP A. 2003. Reproduction of Echium vulgare L. (Boraginaceae) at contaminated sites. ActaBiologica Cracoviensia Series Botanica 45(1): 69–75.
• KELLY JK, RASCH A, and KALISZ S. 2002. A method to estimate pollen viability from pollen size variation. AmericanJournal of Botany 89(6): 1021–1023.
• KHATUN S, and FLOWERS TJ. 1995. The estimation of pollen viability in rice. Journal of Experimental Botany 46(1):151–154.
• KLEIN M. 2000. Pollen studies in plant breeding and selection. Botanical Guidebooks 24: 171–193.
• KŁOSOWSKA K, IZMAIŁOW R, and MUSZYŃSKA E. 2009. Wpływ warunków skażonego siedliska poboczy szlaków komunikacyjnychna procesy embriologiczne u wybranychgatunków roślin (Lotus corniculatus L. i Lepidium ruderaleL.). Journal of Elementology 14, suppl.: 47–48.
• KOŚCIŃSKA-PAJĄK M. 2000. Microspores and pollen grain in triploid Chondrilla juncea L. from unpolluted and pollutedareas. Acta Biologica Cracoviensia Series Botanica42(2): 135–140.
• LANKINEN A. 2001. In vitro pollen competitive ability in Viola tricolor: temperature and pollen donor effects. Oecologia128: 492–498.
• LANKINEN A. 2008. Root competition influences pollen competitive ability in Viola tricolor: effects of presence of a competitorbeyond resource availability? Journal of Ecology96(4): 756–765.
• MALAYERI BE, CHEHREGANI A, MOHSENZADE F, and GOLMOHAMMADI R. 2005. Effect of heavy metals on the developmental stagesof ovule and embryonic sac in Euphorbia cheiradenia.Pakistan Journal of Biological Science 8(4): 622–625.
• MELSER C, and KLINKHAMER PG. 2001. Selective seed abortion increases offspring survival in Cynoglossum officinale(Boraginaceae). American Journal of Botany 88(6):1033–1040.
• MIČIETA K, and MURIN G. 1996. Microspore analysis for genotoxicity of a polluted environment. Environmental andExperimental Botany 36(1): 21–27.
• NEPI M, and FRANCHI GG. 2000. Cytochemistry of mature angiosperm pollen. Plant Systematics and Evolution 222:45–62.
• OSTROLUCKA MG. 1989. Differentiation of male reproductive organs and oak fertility in urban environment. Biologia(Bratislava) 44(9): 793–799.
• OSTROLUCKA MG, Bolvansky M, and Tokar F. 1995. Vitality of pine pollen (Pinus sylvestris L. and Pinus nigra Arnold) onsites with different ecological conditions. Biologia(Bratislava) 50: 47–51.
• RODRIGUEZ-RIANO T, and DAFNI A. 2000. A new procedure to asses pollen viability. Sexual Plant Reproduction 12(4): 241–244.
• SHIVANNA KR, and RANGASWAMY NS. 1992. Pollen Biology. A Laboratory Manual. Springer Verlag, Berlin, Heidelberg,New York, London, Paris, Tokyo, Hong Kong, Barcelona,Budapest.
• SINGH RJ. 2003. Plant Cytogenetics. 2nd ed. CRC Press, Boca Raton.
• SIUTA A, BOżEK M, JĘDRZEJCZYK M, ROSTAŃSKI A, and KUTA E. 2005. Is the blue zinc violet (Viola guestphalica Nauenb.)a taxon of hybrid origin? – Evidence from embryology. ActaBiologica Cracoviensia Series Botanica 47(1): 237–245.
• SKOGSMYR I, and LANKINEN A. 2000. Potential selection for female choice in Viola tricolor. Evolutionary Ecology Research 2:1–15.
• SŁOMKA A, LIBIK-KONIECZNY M, KUTA E, and MISZALSKI Z. 2008. Metalliferous and non-metalliferous populations of Violatricolor represent similar mode of antioxidative response.Journal of Plant Physiology 165: 1610–1619.
• STACE CA. 1991. Plant Taxonomy and Biosystematics. 2-end ed. Cambridge University Press, Cambridge.
• STUESSY TF. 2009. Plant Taxonomy. The Systematic Evaluation of Comparative Data. Columbia UniversityPress, New York.
• SUN K, CIU Y, and HAUSER BA. 2005. Environmental stress alters genes expression and induces ovule abortion: reactive oxygenspecies appear as ovule commit to abort. Planta 222:632–642.
• TAYLOR LP, and HEPLER PK. 1997. Pollen germination and tube growth. Annual Review of Plant Physiology and PlantMolecular Biology 48: 461–491.
• VIžINTIN L, and BOHANEC B. 2004. In vitro manipulation of Cucumber (Cucumis sativus L.) pollen and microspores:isolation procedures, viability tests, germination, maturation. Acta Biologica Cracoviensia Series Botanica 46:177–183.
• WALKER JW, and DOYLE JA. 1975. The bases of angiosperm phylogeny: Palynology. Annals of the Missouri Botanical Garden 62: 664–723.
• WĘDZONY M. 1996. Mikroskopia Fluorescencyjna dla Botaników. Polska Akademia Nauk, Zakład FizjologiiRoślin im. Franciszka Gorskiego, Kraków.
• WIERZBICKA M, and PANUFNIK D. 1998. The adaptation of Silene vulgaris to the growth on a calamine waste heap (SPoland). Environmental Pollution 101: 415–426.
• YOUSEFI N, CHEHREGANI A, MALAYERI B, LORESTANI B, and CHERAGHI M. 2009. Effect of heavy metals on the developmentalstages of ovule and seed proteins in Chenopodium botrysL. (Chenopodiaceae). Biological Trace Element Research.Doi 10.1007/212011-009-8386-x