Chemical characterisation of methanolic-water extracts from the fruit of acclimated Lycium chinense Mill.

• Autorzy: Noculak-Palczewska A , Matkowski A. , Gasiorowski K. , Tabaka H. , Oszmianski J. , Lamer-Zarawska E.
• Języki publikacji: EN

Abstrakty

EN

Methanolic-water extracts of wolfberry fruit (Lycii Fructus) harvested from plants cultivated in Poland have been analysed chemically to reveal compounds responsible for the biological activity of the crude drug. The potential for the scavenging of free radicals has been tested by means of a DPPH spectrophotometric assay. In the extracts several compounds with potential antioxidant properties have been detected: L-ascorbic acid, tocopherols (a, ß and y), ß-carotene, as well as phenolic acids (gallic, protocatechic, caffeic, chlorogenic, neochlorogenic). A considerable free-radical scavenging activity was confirmed by a DPPH assay, with EC50 of the extract being 24.08 mg/l.

PL

W celu identyfikacji składników biologicznie czynnych analizowano ekstrakty metanolowo-wodne owoców kolcowoju chińskiego (Lycii Fructus), zebrane z roślin aklimatyzowanych w Polsce. Zmiatanie wolnych rodników oszacowano za pomocą kolorymetrycznego testu DPPH. W ekstraktach stwierdzono związki o potencjalnym działaniu antyoksydacyjnym: kwas L-askorbinowy, tokoferole (a, ß i y), ß-karoten oraz kwasy fenolowe: galusowy, protokatechowy, kawowy, chlorogenowy oraz neochlorogenowy. Stwierdzono także dużą aktywność przeciwwolnorodnikową badanych ekstraktów, czego dowodzi EC50 w wysokości 24,08 mg/1.

Słowa kluczowe

EN

plant acclimatization; Chinese wolfberry; chemical compound; vitamin; Lycium chinensis; methanolic-water extract; polyphenol; fruit

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2004
• Tom: 50
• Numer: 1
• Opis fizyczny: p.47-53,fig.,ref.

Twórcy

autor

Noculak-Palczewska A

• Wroclaw Medical University, Jana Kochanowskiego 10, 51-601 Wroclaw, Poland

autor

Matkowski A.

autor

Gasiorowski K.

autor

Tabaka H.

autor

Oszmianski J.

autor

Lamer-Zarawska E.

Bibliografia

• 1. Brand-Williams W, Cuvelier ME, Berset C, Use of a free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 1995; 28:25.
• 2. Cao GW, Yang WG, Du P, Observation of the effects of LAK/II-2 therapy combining with Lycium barbarům polysaccharides in the treatment of 75 cancer patients. Chinese Journal of Oncology 1994; 16:428.
• 3. Cao GW, Influence of Lycium barbarům polysaccharides and interleukin-2 in vivo on the induction of two kinds of LAK cells from aged mice in vitro. Chinese Journal of Microbiology and Immunology 1992; 12:390.
• 4. Chin YW, Lim SW, Kim SH, Shin DY, Suh YG, Kim YB, Kim YC, KimJ, Hepatoprotective pyrrole derivatives of Lycium chinense fruits. Bioorg Med Chem Lett 2003; 13:79.
• 5. Ching LS, Mohamed S, Alpha-tocopherol content in 62 edible tropical plants. J Agric Food Chem 2001; 49:3101.
• 6. Gąsiorowski K, Tabaka H, Działo E, Noculak-Palczewska A, Lamer-Zarawska E, In vitro immunomodulatory activity of methanolic-water extract from Chinese wolfberry fruits (Lycium chinense Mill.). Herba Polonica 2000; 49.
• 7. Han SH, Lee HH, Lee IS, Moon YH, Woo ER, A new phenolic amide from Lycium chinense Miller. Arch Pharm Res 2002; 25:433.
• 8. Huang Y, Tan A, Shen Y, Lu J, Scavenging effect of total flavonoids of Lycium barbarům L. on active oxygen radicals and inhibitory effects on heat output from L1210 cells. Wei Sheng Yanjiu 1998; 27:109.
• 9. Ko RJ, New EnglJ Med 1998; 339:847.
• 10. Maeda Y, Ochi S, Matsui T, Matsubarba S, Liquid chromatographic determination of L-ascorbic acid in candies and soft drinks. J Assoc Anal Chem 1988; 71:502.
• 11. Shin YG, Clio KH, Kim JM, Park MK, Park JH, Determination of betaine in Lycium chinense fruits by liquid chromatography-electrospray ionization mass spectrometry. J Chromatogr A 1999; 857:331.
• 12. Tang W, Eisenbrand G, Chinese drugs of plant origin. Chemistry, pharmacology and use in traditional and modern medicine. Springer Verlag, Berlin 1992.
• 13. Wang YS, Pharmacology and Application of Chinese Materia Medica. People’s Health Publisher, Beijing 1983.
• 14. Wang BK, Xing ST, Zhou JH, Effect of Lycium barbarům polysaccharides on the immune response of the T, CTL and NK cells in normal and cyclophosphamide-treated mice. Chin J Pharmacol Toxicol 1990; 4:39.
• 15. Zhang X, Xie X, Experimental research on the role of Lycium barbarům polysaccharide in antiperoxidation. China Journal of Chinese Materia Medica 1993; 18:110.
• 16. Yin J, Guo LG, Modern Research and Clinical Applications of Chinese Materia Medica. Academic Publishers, Beijing 1993.
• 17. Zhu YP, Chinese Materia Medica, Chemistry, Pharmacology and Applications. Harwood Academic Publishers, Amsterdam 1998.