PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
1998 | 50 | 3-4 |

Tytuł artykułu

Ocena przydatnosci wybranych systemow komercyjnych do identyfikowania ziarenkowcow z rodzaju Staphylococcus

Warianty tytułu

Języki publikacji

PL

Abstrakty

PL
Porównano 4 komercyjne systemy służące do identyfikacji gronkowców zarówno koagulazo-ujemnych, jak i koagulazo-dodatnich. Stwierdzono, że najbardziej przydatnym z ocenianych systemów był zestaw ID 32 STAPH.
EN
The aim of the study was the assessment of the usefulness of commercially available systems for rapid identification of staphylococci. API STAPH (bioMerieux, France), ID 32 STAPH (bioMerieux, France), GPL (HTL, Poland) and Staph-Zym (Rosco, Denmark). The identifications were carried out according to producer's instruction. The material for the study comprised 76 staphylococcal strains, coagulase-positive and coagulase-negative. The strains were isolated from throat, nasal, wound, bone slivers, pus and blood of inpatients and from throat and nasal swabs of outpatients. Besides that, for the study staphylococcal strains were obtained from the American Collection of Typical Cultures (ATCC) and from the Polish Collection of Microorganisms (PCM). All tested strains were identified on the basis of classic biochemical tests. In the light of obtained results it is concluded that the commercial system most suitable for identification of staphylococci was ID 32 STAPH (bioMerieux), which has a wide spectrum of species identifiable with it and the highest percent (95%) of correctly identified species. The least suitable system was the GPL 15 (HTL, Poland).

Wydawca

-

Rocznik

Tom

50

Numer

3-4

Opis fizyczny

s.161-169,rys.,tab.,bibliogr.

Twórcy

  • Panstwowy Zaklad Higieny, 00-791 Warszawa, ul.Chocimska 24
autor

Bibliografia

  • 1.Bergey’s Manual of Determinative Bacteriology (JG Holt, R Krieg, PHA Sneath, JT Staley, ST Williams eds) Wydanie 9, Williams & Wilkins, Baltimore, 1994; 532-51.
  • 2. Herchline TE, Ayers LW. Occurence of Staphylococcus lugdunensis in consecutive clinical cultures and relationship of isolation to infection. J Clin Microbiol 1991; 29: 419-21.
  • 3. Ieven M, Verhoeven J, Pattyn SR, Goossenes H. Rapid and economical method for species identification of clinically significant coagulase-negative Staphylococci. J Clin Microbiol 1995; 33: 1060-63.
  • 4. Jeljaszewicz J, Cybulska J, Dziarski R i inni. Ziarenkowce Gram-dodatnie: biologia, rozpoz¬nanie i różnicowanie, Wydanie metodyczne PZH Warszawa 1978.
  • 5. Kamath U, Singer C, Isenberg HD. Clinical significance of Staphylococcus warneri bacteremia. J Clin Microbiol 1992; 30: 261-64
  • 6. Kloos WE, Schleifer KH. Simplified scheme for routine identification of human Staphylococ¬cus species. J Clin Microbiol 1975; 1: 82-88.
  • 7. Kloos WE, Bannerman TL. Update on clinical significance of coagulase-negative Staphylo¬cocci. Clin Microbiol Rev 1994; 7: 117-40.
  • 8. Kloos WE, Bannerman TL. Staphylococcus and Micrococcus. W: Manual of Clinical Micro¬biology, (PR Murray, EJ Baron, MA Pfaller, FC Tenover, RH Yolken eds.) Wydanie 6-te. ASM Washington D.C. 1995, 282-98.
  • 9. Mulder JG. A simple and inexpensive method for the identification of Staphylococcus epidermidis and Staphylococcus hominis. Eur J Cli Microbiol Infect Dis 1995; 14: 1052-56.
  • 10. Pratt-Rippin K, Pezzlo M. Identification of commonly isolated aerobic Gram-positive bacteria. W: Clinical Microbiology Procedures Handbook, ed. H D Isenberg, 1994, 1.20.1.-11.
  • 11. Renneberg J, Rieneck K, Gutschik E. Evaluation of Staph-Zym system for identification of coagulase-negative Staphylococci. J Clin Microbiol 1995; 33: 1150-53.
  • 12. Rupp ME, Archer GL. Coagulase-negative Staphylococci: pathogens associated with medical progress. Clin Infect Dis 1994; 19: 232-45.
  • 13. Vermont CL, Hartwig NG, Fleer A i inni. Persistance of clones of coagulase-negative Stap¬hylococci among premature neonates in neonatal intensive care units: two-ceter study of bacterial genotyping and patient risk factors. J Clin Microbiol 1998; 369: 2485-90.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-ed2e5bd7-1ee6-455a-a57a-94a953842e40
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.