The effect of triethyllead on the motile activity of Walker 256 carcinosarcoma cells

• Autorzy: Sroka J , Kaminski R. , Michalik M. , Madeja Z. , Przestalski S. , Korohoda W.
• Języki publikacji: EN

Abstrakty

EN

The effect of triethyllead (TriEL) on the morphology and motile activity of Walker 256 carcinosarcoma cells was investigated. It was found that both 2 and 5 μM TriEL affected the cellular motility in a dose- and time- dependent manner. Initially, 2 μM TriEL caused the formation of blebs instead of lamellipodia at the front of some cells and stimulated the migration of Walker cells, but after 2 hours of 2 μM TriEL treatment, a reduction of cellular motility was observed. In the presence of 5 μM TriEL, Walker 256 carcinosarcoma cells rounded up, and their rate of movement was reduced. Moreover, the treatment of Walker carcinosarcoma cells with TriEL caused the disruption of microtubules and affected the F-actin distribution at both concentrations. At a concentration of 2 μM TriEL, the actin staining intensity was greatest in the tail of front-tail polarised blebbing cells and the actin layer was very thin at the leading edge. The control cells showed linear cortical F-actin distribution and somewhat less intense cytoplasmic staining at the same TriEL concentration. Cells treated with 5 μM TriEL showed an under-membrane pattern of actin distribution.

Słowa kluczowe

EN

microtubule; Walker 256 carcinosarcoma cell; motile activity; cell migration; F-actin; triethyllead

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2004
• Tom: 09
• Numer: 1
• Opis fizyczny: p.15-30,fig.,ref.

Twórcy

autor

Sroka J

• Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland

autor

Kaminski R.

autor

Michalik M.

autor

Madeja Z.

autor

Przestalski S.

autor

Korohoda W.

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