Structural and functional changes of bovine carbonic anhydrase as a consequence of temperature

• Autorzy: Sarraf N. S. , Saboury A.A. , Ranjbar B. , Moosavi-Movahedi A.A.
• Języki publikacji: EN

Abstrakty

EN

The temperature dependence of the activity and structure of the enzyme carbonic anhydrase was studied. The Arrhenius plot shows a jump which is seen usually in proteins with more than one subunit or with one subunit but more than one domain. Since carbonic anhydrase has only one subunit with one domain, the fine conformational changes of the protein motifs could only be detected through circular dichroism polarimetry. It seems that the jump in Arrhenius plot is a result of some slight structural changes in the secondary and tertiary structures of the enzyme.

Słowa kluczowe

EN

temperature; functional change; enzyme carbonic anhydrase; bovine carbonic anhydrase; structural change; circular dichroism; Arrhenius plot; activation energy

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2004
• Tom: 51
• Numer: 3
• Opis fizyczny: p.665-671,fig.,ref.

Twórcy

autor

Sarraf N. S.

• University of Tehran, Tehran, Iran

autor

Saboury A.A.

autor

Ranjbar B.

autor

Moosavi-Movahedi A.A.

Bibliografia

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