Abnormal meiosis in tetraploid genotypes of Brachiaria brizantha [Poaceae] induced by colchicine: its implications for breeding

• Autorzy: Mendez-Bonato A B , Ferrari Felisimo M , Souza Kaneshima A M , Pessim C , Calisto V , Suely Pagliarini M , Borges do Valle C
• Języki publikacji: EN

Abstrakty

EN

Meiotic behavior was analyzed in 6 progenies from 3 artificially induced tetraploid (2n = 4x = 36) sexual genotypes (C31, C41, and C48) of the normally apomictic Brachiaria brizantha (Hochst, ex A. Rich.) Stapf., syn. Urochloa brizantha (Hochst, ex A. Rich.) R. Webster. These are key plants to allow intraspecific hybridization of this important forage species, widely used for pastures in the tropics. The percentage of abnormal cells among the plants ranged from 39.8% to 63.2%. In the single plant derived from C48, only the common meiotic abnormalities typical of polyploids were observed, while in plants derived from C31 and C41, a distinct behavior was found. In the majority of cells of those plants, the chromosomes remained scattered in the cytoplasm in the first division, without forming a metaphase plate. This abnormality blocked chromosome movements at anaphase I. Several micronuclei of various sizes were formed and, after the occurrence of an irregular first cytokinesis, the meiocytes progressed normally to the second division, generating polyads with unbalanced microspores. Pollen viability was not correlated with meiotic abnormalities. The importance of these findings to the Brachiaria breeding program is discussed. The sexual progeny of C48 seems most suitable as female parents to be used in intra- and interspecific hybridization.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2009
• Tom: 50
• Numer: 2
• Opis fizyczny: p.83-87,fig.,ref.

Twórcy

autor

Mendez-Bonato A B

• Department of Cell Biology and Genetics, State University of Maringa, 87020-900 Maringa-Parana, Brazil

autor

Ferrari Felisimo M

• Department of Cell Biology and Genetics, State University of Maringa, 87020-900 Maringa-Parana, Brazil

autor

Souza Kaneshima A M

• Department of Cell Biology and Genetics, State University of Maringa, 87020-900 Maringa-Parana, Brazil

autor

Pessim C

• Department of Cell Biology and Genetics, State University of Maringa, 87020-900 Maringa-Parana, Brazil

autor

Calisto V

• Department of Cell Biology and Genetics, State University of Maringa, 87020-900 Maringa-Parana, Brazil

autor

Suely Pagliarini M

• Department of Cell Biology and Genetics, State University of Maringa, 87020-900 Maringa-Parana, Brazil

autor

Borges do Valle C

• Embrapa Beef Cattle, Campo Grande MS, Brazil

Bibliografia

• [ABIEC] Associaçăo Brasileira das Indústrias Exportadoras de Carne, 2008. http/www.abiec.com.br/institutional.asp. Cited on March 11, 2008.
• Adamowski EV, Pagliarini MS, Valle CB, 2008. Meiotic behavior in three interspecific three way hybrids between Brachiaria ruziziensis and B. brizantha (Poaceae: Paniceae). J Genet 87: 33-38.
• Araujo ACG, Nóbrega JM, Pozzobon MT, Carneiro VTC, 2005. Evidence of sexuality in induced tetraploids of Brachiaria brizantha (Poaceae). Euphytica 144: 39-50.
• Ferguson JE, Crowder LV, 1974. Cytology and breeding behaviour of Brachiaria ruziziensis. Crop Sci 14: 893-895.
• Fuzinatto VA, Pagliarini MS, Valle CB, 2007. Microsporogenesis in sexual Brachiaria hybrids (Poaceae). Genet Mol Biol 6: 1107-1117.
• Gobbe J, Swenne A, Louant BP, 1981. Diploďdes naturels et autotétraplod’des induits chez Brachiaria ruziziensis Germain et Evrard: critčres d’identification. Agron Trop 36: 339-346.
• Keller-Grein G, Maass BL, Hanson J, 1996. Natural variation in Brachiaria and existing germplasm collections. In: Miles JW, Maass BL, Valle CB, eds. Brachiaria: biology, agronomy, and improvement. Colombia: CIAT/Embrapa: 17—42.
• Mendes-Bonato AB, Junqueira Filho RG, Pagliarini MS, Valle CB, Penteado MIO, 2002a. Unusual cytological patterns of microsporogenesis in Brachiaria decumbens: abnormalities in spindle and defective cytokinesis causing precocious cellularization. Cell Biol Int 26: 641-646.
• Mendes-Bonato AB, Pagliarini MS, Forli F, Valle CB, Penteado MIO, 2002b. Chromosome number and microsporogenesis in Brachiaria brizantha (Gramineae). Euphytica 125: 419—425.
• Mendes-Bonato AB, Pagliarini MS, Risso-Pascotto C, Valle CB, 2006. Chromosome number and meiotic behavior in Brachiaria jubata (Gramineae). J Genet 85: 83-87.
• Miles JW, Valle CB, 1991. Assessment of reproductive behavior of interspecific Brachiaria hybrids. Apomixis Newslett 3: 9-10.
• Miles JW, Valle CB, Rao IM, Euclides VPB, 2004. Brachiaria grasses. In: Sollenberger L. editor. Warm-Season (C4) Grasses Madison: ASA, CSSA, SSSA: 745-760.
• Pagliarini MS, Risso-Pascotto C, Souza- Kaneshima AM, Valle CB, 2008. Analysis of meiotic behavior in selecting potential genitors among diploid and artificially induced tetraploid accessions of Brachiaria ruziziensis (Poaceae). Euphytica 164: 181-187.
• Penteado MIO, Santos ACM, Rodrigues IF, Valle CB, Seixas MAC, Esteves A, 2000. Determinaçăo de poliploidia e avaliaçăo da quantidade de DNA total em diferentes espécies de Brachiaria. Boletim de Pesquisa No. 11. Campo Grande-MS, Embrapa Gado de Corte: 19.
• Pinheiro AA, Pozzobon MT, Valle CB, Penteado MIO, Carneiro VTC, 2000. Duplication of the chromosome number of diploid Brachiaria brizantha plants using colchicine. Plant Cell Rep 19: 274-278.
• Risso-Pascotto C, Pagliarini MS, Valle CB, 2005a. Multiple spindles and cellularization during microsporogenesis in an artificially induced tetraploid accession of Brachiaria ruziziensis (Gramineae). Plant Cell Rep 23: 522-527.
• Risso-Pascotto C, Pagliarini MS, Valle CB, 2005b. Meiotic behavior in interspecific hybrids between Brachiaria ruziziensis and Brachiaria brizantha (Poaceae). Euphytica 145: 155-159.
• Risso-Pascotto C, Pagliarini MS, Valle CB, 2006. Microsporogenesis in Brachiaria dictyoneura (Fig. & De Not.) Stapf (Poaceae: Paniceae). Genet Mol Res 5: 837-845.
• Swenne A, Louant BP, Dujardin M, 1981. Induction par la colchicine de formes autotétraplod’des chez Brachiaria ruziziensis Germain et Evrard (Graminée). Agron Trop 36: 134-141.
• Taschetto OM, Pagliarini MS, 1993. Description of a new type of meiotic abnormality in maize (Zea mays L.). Maydica 8: 47-50.
• Utsunomiya KS, Pagliarini MS, Valle CB, 2005. Microsporogenesis in tetraploid accessions of Brachiaria nigropedata (Ficalho & Hiern) Stapf (Gramineae). Biocell 29: 295-301.
• Valle CB, Savidan YH, 1996. Genetics, cytogenetics, and reproductive biology of Brachiaria. In: Miles JW, Maass BL and Valle CB, eds. Brachiaria: biology, agronomy, and improvement, Colombia: CIAT/Embrapa: 147-163.
• Young BA, Sherwood RT, Bashaw EC, 1979. Cleared-pistil and thick-sectioning techniques for detecting aposporous apomixis in grasses. Can J Bot 57: 1668-1672.