Developmental abnormalities in chicken embryos after injection proteolysis inhibitors from Ascaris suum

• Autorzy: Blaszkowska J
• Języki publikacji: EN

Abstrakty

EN

It bas been found that trypsin and α-chymotrypsin inhibitors isolated from Ascaris suum act embryotoxically and teratogenically on White Leghorn clucken embryos. Mortatity rate for the chicken embryos on day 15 of incubation was 45.0 ± 3.5% after injection of trypsin inbibitor and 44.0 ± 3.5% after adminstration α-chymotrypsin inbibitor. Gross examination of surviving embryos and their dissection revealed pathological changes (abdominal dropsy, umbilical hernia, subcutaneous oedema, hemoperitoneum, hemopericardium), symptoms indicating retardation in growth (lack of down, retarded ossification of long bones, decreased mean body weight) as well as malformations (schistocelia, micrognathia, cyclopia, crossed beak, cranial deformities) after injection of inhibitors from Ascaris. The highest incidence of embryos with pathological changes and malformations was found after administralion of α-chymotrypsin inbibitor. The most commonly occurring abnormality was schistocelia (21.4 ± 3.88%). Growth malformations were not found in the control groups. The trypsin and α-chymotrypsin inhibitors present in Ascaris homogenate have a significant disturbing effect on the development of the chicken embryo.

Słowa kluczowe

EN

Ascaris suum; teratogen; proteolysis inhibitor; developmental abnormality; inhibitor; chicken embryo; injection

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 2002
• Tom: 48
• Numer: 1
• Opis fizyczny: p.55-64,fig.,ref.

Twórcy

autor

Blaszkowska J

• Medical University, Kosciuszki str.85, 90-436 Lodz, Poland

Bibliografia

• Abuereish G.M., Peanasky R.J. 1974. Pepsin inhibitors from Ascaris lumbricoides. Isolation, purification and some properties. Journal of Biological Chemistry 249: 1558-1565.
• Becker M.M., Harrop S.A., Dalton J.P., Kalina B.H., Mcmanus D.P., Brindley P.J. 1995. Cloning and characterization of the Schistosoma japonicum aspartic proteinase involved in hemoglobin degradation. Journal of Biological Chemistry 270: 2449-34501.
• Błaszkowska J. 1998. The effect of alpba-chymotrypsin inhibitor from Ascaris lumbricoides suum oo chick embryo. Wiadomości Parazytologiczne 44: 63-71.
• Błaszkowska J. 1999a. Disturbances of mouse pregnancy after injection of Ascaris trypsin inhibitor during early organogeoesis. Zoologica Poloniae 44: 23-36.
• Błaszkowska J. 1999b. The effect of Ascaris tegumental homogeoate aod α-chymotrypsin inhibitor isolated from the nematode on mouse pregnancy. Helminthologia 36: 225-234.
• Błaszkowska J. 2001. Disturbances of mouse pregnancy after injection of Ascaris α-chymotrypsin inhibitor during early organogenesis. Helminthologia 38: 15-21.
• Clinton W.A., Baig J.S., Robinson P. 1996. Taenia saginata oncospher excretory/secretory peptidases. Journal of Parasitology 82: 7- 10.
• Devi C.M., Raj R.K., 1996. Histopathological changes in the spleen of BALB/c mice caused by excretory-secretory proteions of Setaria digitata (Von Listow). Indian Journal of Experimental Biology 34: 32-36.
• Dawson A.A. 1976. A note oo the staining of the skeleton of cleared specimens with alizarin red S. Stain Technology 1: 123-124.
• Edrington T.S., Harvey R.B., Kubena L.F. 1995. Toxic effects of aflatoxin B1 and ochratoxin A, alone and in combination, on chicken embryos. Bulletin Environmental Contamination and Toxicology 54: 331-336.
• Gebhardt D.O. 1972. The use of chick embryo in applied teratology. Advances of Teratology 5: 97-111.
• Gimwood I., Smith J.E. 1966. Toxoplasma gondii: the role of parasite surface and secreted proteins in host cell invasion. International Journal of Parasitology 26: 169-173.
• Grasberger D.L., Clore G.M., Gronenborn A.M. 1994. High-resolution structure of Ascaris trypsin inbibitor in solution: direct evidence for a pH-induced conformational transtition in the reactive site. Structure 2: 669-678.
• Grutter M.G. 1994. Proteinase inhibitors: another new fold. Structure 2: 575-576.
• Hammerberg B.C., Dangler C., Williams J.F. 1980. Taenia taeniaeformis-chemical composition of parasite factory affecting coagulation and complement cascades. Journal of Parasitology 66: 569-576.
• Huang K., Strynadka C.I., Bernard V.B., Peanasky R.J., James N.G. 1994. The molecular structure of the complex of Ascaris chymotrypsin/elastase inhibitor with porcine elastase. Structure 2: 679-689.
• Illanes J., Blanquez M.J., Gonzuale M.E., Rojo C. 1995. Induccion experimental a malformaciooes por instilacion de etanol en huevos embrionados de Pollo (Gallus gallus). Anatomy, Histology, Embryology 24: 217-221.
• Juhasz S. 1979. Proteolytic enzymes and enzymes inhibitors in Ascaris summ. II. Protease inhibitors produced in vivo in enzyme-free medium. Acta Veterinaria Academiae Scientiarum Hungaricae 27: 89-91.
• Juhasz S., Matskasi J. 1979. Proteolytic enzymes and enzymes inbibitors in Ascaris suum. III. In vitro study of proteinase inactivation by the parasite. Acta Veterinaria Academiae Scientiarum Hungaricae 27: 93-96.
• Kadłubowski R., Błaszkowska J. 1988. Proteases and their inhibitors in the host-parasite system. VIII. The effect of Ascaris tegumental homogenate and trypsin inhibitor isolated from the nematode on the number of foetuses per litter in mice. Acta Parasitologica Polonica 33: 285-293.
• Kadłubowski R., Błaszkowska J. 1992. Dzialanie homogenatu Ascaris lumbricoides suis oraz jej inbibitora trypsyny na zarodek kury. Wiadomości Parazytologiczne 38: 31-41.
• Karnofsky D.A., Lacon C.R. 1966. The effecf of 1-ß-arabino-furano-sylcytosine on the developing chick embryo. Biochemical Pharmacology 15: 1435-1442.
• Komarek: V., Lemez L. 1986. Anatomia avium domestcarum et embryologia galli. Bratislavia, Priroda, 1-288.
• Kozłowicz S. 1974. Zmiany patomorfologiczne w trzustce w przebiegu glistnicy jelita u świni domowej (Sus domestica). Wiadomości Parazytologiczne 22: 524-533.
• Loutz-Ostertag I. 1966. Action de l'extrait de Toxoplasma (toxoplasmine) sur le developpment embryonnaire du poluet. Archives d. Anatomie Macroscopique 55: 363-386.
• Martzen M.R., Geise G.L., Hogan R.J., Peanasky R.J. 1985. Ascaris summ: localization of the host proteases and parasite proteinase inbibitors in cross section using immunochemical and fluorescent probe technique. Experimental Parasitology 60: 139-149.
• Mclaughlin J., Morliac I. P. 1963. The injection of chemicals into the yolk sac of fertile eggs prior to incubation as a toxicity test. Toxicology and Applied Pharmacology 5: 760-771.
• Peters P.W. 1977. Duble staining of fetal skeletons for cartilage and bone, In: Neubert, D., Merker, H.J. and Kwasigroch, T. E (Eds) Methods in Prenatal Toxicology. Stuttgart, Georg Thieme Publisher, 153-154.
• Rola F.H., Pudles J. 1966. Studies on the chymotryptic inhibitor from Ascaris lumbricoides suum, purification and properties. Archives of Biochemistry and Biophysics 13: 134-142.
• Romanoff A.L 1960. The Avian Embryo. New-York, The Macmillan Company, pp. 1-450.
• Rouyan G.S., Meisel-Mikolajczyk F., Rumin W. 1994. The toxicity of antigenes extracted from strains of Bacteroides vulgatus from different origin to chick embryos. Acta Microbiologica Polonica 43: 97-103.
• Verrett N., Scot W.F. 1980. Toxicity and teratogenicity of food additive chemicals in the developing chicken embryo. Toxicology and Applied Pharmacology 56: 265-273.
• Wilson J.G. 1975. Reproduction and teratogenesis: current method and suggested improvements. Journal of the Association Analytical Chemistry 58: 657-667.