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1998 | 20 | 4 |

Tytuł artykułu

Effect of plant growth regulators and different nitrogen sources on NADP-isocitrate dehydrogenase activity of radish cotyledons

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
NADP-isocitrate dehydrogenase (NADP-ICDH) activity of radish (Raphanus sativus L.) seedlings pretreated with various plant growth regulators: KiN, GA, and ABA and different nitrogen sources viz. KNO₃, NH₄Cl and NH₄NO₃ in light and dark was investigated. ICDH activity was significantly higher in light than in dark; addition of different nitrogen sources reduced it to a greater extent in NO₃ supplementation. Among hormonal treatment only KiN showed slight promotion with KNO₃ and NH₄NO₃. On the other hand in light KNO₃ and/or NH₄NO₃ promoted ICDH activity and among hormones, KiN significantly promoted the activity in KNO₃ and NH₄NO₃ supplemented seedlings while ABA was effective in NH₄CL. It is suggested that in non-photosynthetic tissues, NADP-ICDH provides both reductant and carbon skeleton for glutamate synthesis.

Wydawca

-

Rocznik

Tom

20

Numer

4

Opis fizyczny

p.353-357,fig.

Twórcy

autor
  • Saurashtra, Rajkot 360 005, India
autor
autor

Bibliografia

  • Attucci S., Rivoal J., Brouquisse R., Carde J.P., Pradet A. and Raymond P. 1994. Characterization of a mitochondrial NADP-dependent isocitrate dehydrogenase in axes of germinating sunflower seeds. Plant Sci. 102:49–59.
  • Chanda S.V. and Singh Y.D. 1996. Biochemical analysis of developing wheat grains. J. Agron. Crop Sci. 176:131–139.
  • Chen R.D. and Gadal P. 1990. Do the mitochondria provide the 2-oxoglutarate needed for glutamate synthesis in higher plant chloroplasts? Plant Physiol. Biochem. 28:141–145.
  • Chen R.D., Le Marechal P., Vidal J., Jacquot J.P. and Gadal P. 1988. Purification and comparative properties of the cytosolic isocitrate dehydrogenase (NADP) from pea (Pisum sativum) roots and green leaves. Eur. J. Biochem. 175:565–572.
  • Chen R.D., Bismuth E., Champigny M.L. and Gadal P. 1989. Chromatographic and immunological evidence that chloroplastic and cytosolic pea (Pisum sativum L.) NADP-isocitrate dehydrogenases are distinct isoenzymes. Planta 178:153–163.
  • Doddema H. and Telkamp G.P. 1979. Uptake of nitrate by mutants of Arabidopsis thaliana, disturbed in uptake or reduction of nitrate II kinetics. Physiol. Plant. 45:332–338.
  • Fieuw S., Muller-Robert B., Galvez S. and Willmitzer L. 1995. Cloning and expression analysis of the cytosolic NADP⁺-dependent isocitrate dehydrogenase from potato. Plant Physiol. 107:905–913.
  • Hanning I. and Heldt H.W. 1993. On the function of mitochondrial metabolism during photosynthesis in spinach (Spinacia oleracea) leaves: Partitioning between respiration and export of redox equivalents and precursor for nitrate assimilation products. Plant Physiol. 103:1147–1154.
  • Joy K.W. 1988. Ammonia, glutamine and asparagine: a carbon-nitrogen interface. Can. J. Bot. 66:2103–2109.
  • Miflin B.J. and Lea P.J. 1980. Ammonia assimilation. In The Biochemistry of Plants A Comprehensive Treatise Vol.5 Amino Acids and Derivatives Stumpf P.K. and Conn E.E. eds. Academic Press, New York, 169–202.
  • Osmani S.A. and Scrutton M.C. 1985. The subcellular localization and regulation properties of pyruvate carboxylase from Rhizopus arrhizus. Eur. J. Biochem. 147: 119–128.
  • Preiss J., Robinson N., Spilatro S. and Ncnamara K. 1985. Starch biosynthesis and its regulation. In Regulation of carbon partitioning in photosynthetic tissue. Heldt R.L. and Preiss J. eds. Waverly Press Baltimore M.D. 140–172.
  • Turpin D.H., Elrifi I.R., Birch D.G., Weger H.G. and Holmes J.J. 1988. Interactions between photosynthesis, respiration and nitrogen assimilation in microalgae. Can. J. Bot. 66:2083–2097.
  • Turpin D.H., Vanlerberghe G.C., Amory A.M. and Guy R.D. 1991. The inorganic carbon requirements for nitrogen assimilation. Can. J. Bot. 69:1139–1145.
  • Weger H.H. and Turpin D.H. 1989. Mitochondrial respiration can support NO₃ and NO₂/− reduction during photosynthesis:Interactions between photosynthesis, respiration and N assimilation in the N-limited green algae Selenastrum minutum. Plant Physiol. 89:409–415.
  • Woo K.C., Boyle F.A., Flugge U.T. and Heldt H.W. 1987. ¹⁵N-ammonia assimilation, 2-oxoglutarate transport, and glutamate export in spinach chloroplasts in the presence of decarboxylates in the light. Plant Physiol. 85:621–625.
  • Yamazaki R.K. and Tolbert N. 1970. Enzymatic characterization of leaf peroxisomes. J. Biol. Chem. 245:5137–5144.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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