Applying liquid chromatography with fluorescence detection to determine gentamicin

• Autorzy: Kowalczuk D , Pietras R. , Paw B. , Czerkies A.
• Języki publikacji: EN

Abstrakty

EN

A simple and sensitive procedure for the determination of gentamicin is presented, based on reaction with o-phthaldialdehyde (OPA) in combination with N-acetylcysteine (NAC) in a basic medium. The use of N-acetylcysteine instead of liquid mercaptans for the formation of isoindole derivatives is a good solution for avoiding the unpleasant odour of thiol compounds. Determination of the resulting fluorescent derivatives was carried out by reversed-phase chromatography at λex=328 nm and λem=423. Stability of OPA/NAC/gentamicin-conjugate was improved by the addition of hydroxypropyl-β-cyclodextrin. The calibration graph was linear (r = 0.9997) over the range 0.4-12.8 μg mL-1. The precision (RSD%) of the method varied from 1.5 to 8.5. The method was satisfactorily applied to the determination of gentamicin in pharmaceutical preparations with a mean recovery of 100.02% for injections and 88.94% for drops.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2010
• Tom: 19
• Numer: 3
• Opis fizyczny: p.587-591,fig.,ref.

Twórcy

autor

Kowalczuk D

• Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland

autor

Pietras R.

autor

Paw B.

autor

Czerkies A.

Bibliografia

• 1. AL-AMOUD A., CLARK B., CHRYSTYN H. Determination of gentamicin in urine samples after inhalation by reversed-phase high-performance liquid chromatography using pre-column derivatisation with o-phthalaldehyde. J Chromatogr B Analyt Technol Biomed Life Sci. 769, 89, 2002.
• 2. ARCELLONI C., COMUZZI B., VAIANI R., PARONI R. Quantification of gentamicin in Mueller-Hinton agar by high-performance liquid chromatography. J Chromatogr B Biomed Sci Appl. 753, 151, 2001.
• 3. FERNANDEZ-RAMOS J., GARCIA-CAMPANA A., ALES-BARRERO F., BOSQUE-SENDRA, J. Determination of gentamicin in pharmaceutical formulations using peroxy oxalate chemiluminescent detection in flow-injection analysis. Talanta. 69, 763, 2006.
• 4. KIM B., LEE S., LEE H., OK J. Reversed-phase liquid chromatographic method for the analysis of aminoglycoside antibiotics using pre-column derivatization with phenylisocyanate. Biomed Chromatogr. 17, 396, 2003.
• 5. LECAROZ C., CAMPANERO M., GAMAZO C., BLANCO-PRIETO M. Determination of gentamicin in different matrices by a new sensitive high-performance liquid chromatography-mass spectrometric method. J Antimicrob Chemother. 58, 557, 2006.
• 6. POSYNIAK A., ZMUDZKI J., NIEDZIELSKA J. Sample preparation for residue determination of gentamicin an d' neomycin by liquid chromatography. J Chromatogr A. 914, 59, 2001.
• 7. STEAD D.A., RICHARDS R. Sensitive fluorimetric determination of gentamicin sulfate in biological matrices using solid-phase extraction, pre-column derivatization with 9-fluorenylmethyl chloroformate and reversed-phase high-performance liquid chromatography. J Chromatogr B Biomed Appl. 675, 295, 1996.
• 8. VOGEL R., DEFILLIPO K., REIF V. Determination of isepamicin sulfate and related compounds by high performance liquid chromatography using evaporative light scattering detection. J Pharm Biomed Anal. 24, 405, 2001.
• 9. YANG H., ZHU Q., QU H., CHEN X., DING M., XU J. Flow injection fluorescence immunoassay for gentamicin using sol-gel-derived mesoporous biomaterial. Anal Biochem. 308, 71, 2002