Cuticle fluorescence during embryogenesis of triticale [xTriticosecale Wittm.]

• Autorzy: Szczuka E. , Szozda A. , Wedzony M. , Gielwanowska I. , Bednara J.
• Języki publikacji: EN

Abstrakty

EN

A cuticle visualized by auramine O fluorescence on the surface of developing triticale (xTriticosecale Wittm.) embryos was investigated. The localization of the cuticle was studied on zygotic and somatic embryos derived from androgenic culture. In triticale zygotic embryogenesis, a fluorescing cuticle layer appears on the globular embryo and persists during successive stages of development. The cuticle is especially thick on the tip of the coleoptile, the epiblast, the hypoblast and shoot apex surfaces adjacent to the endosperm. During maturation of the embryos there is no fluorescing cuticular layer on the scutellum surface. The fluorescing cuticle covers the whole surface of the mature embryos. In a similar way, the fluorescing cuticle invests whole globular and older somatic triticale embryos, while its layer is not always continuous at later stages. The cuticle persists also on the surface of plantlets growing from somatic embryos. In this paper the influence of the cuticle on embryo polarization, nutrition and morphogenesis is discussed.

Słowa kluczowe

EN

electron microscopy; cuticle; triticale; Triticosecale; zygotic embryogenesis; morphogenesis; anther culture; embryo polarization; angiosperm; fluorescence; androgenic culture; somatic embryogenesis; embryo

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 1999
• Tom: 41
• Opis fizyczny: p.169-175,fig.

Twórcy

autor

Szczuka E.

• Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland

autor

Szozda A.

autor

Wedzony M.

autor

Gielwanowska I.

autor

Bednara J.

Bibliografia

• Chamberlin MA, Horner HT, and Palmer GR. 1993. Nutrition of ovule, embryo sac and young embryo in soybean: an anatomical and autoradiographic study. Canadian Journal of Botany 71: 1153-1168.
• Chu CC, Hill RD. 1988. An improved anther culture method for obtaining higher frequency of pollen embryoids in Triticum aestivum L. Plant Science 55: 175-181.
• D‘Alascio-Deschamps R. 1978. Etude ultrastructurale et cytochimique de la jonction albumen - embryon pour divers stades du development. Societatis Botanicorum Francae, Actualites Botanicorum 1/2: 273-277.
• Fry SC. 1990. Roles of the primary cell wall in morphogenesis. In: Nijkamp HJJ, van der Plas LHW and van Aartrijk J [eds.], Progress in plant cellular and molecular biology, 504-513. Kluwer Academic Press, Dordrecht, The Netherlands.
• Heslop-Harrison Y. 1977. The pollen-stigma interaction: pollen tube penetration in Crocus. Annals of Botany 41: 913-922.
• Lukowitz W, Mayer U, and Jürgens G. 1996. Cytokinesis in the Arabidopsis embryo involves the syntaxin-related KNOLLE gene product. Cell 84: 61-71.
• Newcomb W. 1973. The development of the embryo sac of sunflower Helianthus annuus after fertilization. Canadian Journal of Botany 51: 879-890.
• Rodkiewicz B, Szczuka E. 1994. Chlorophyll and cutin in early embryogenesis in Capsella, Arabidopsis, and Stellaria investigated by fluorescence microscopy. Sexual Plant Reproduction 7: 287-289.
• Szczuka E, Rodkiewicz B. 1996. A cutin fluorescence pattern in developing embryos of some Angiosperms. Acta Societatis Botanicorum Poloniae 65 (1-2): 155-160.
• Szczuka E, Fyk B, and Rodkiewicz B. 1995. Cutin and chlorophyll fluorescence in developing embryos of some Angiosperms. Platinum Jubilee Volumen. Journal Indian Botanicorum Societatis. 74A: 273-282.
• Timmers ACJ. 1993. Imaging of polarity during zygotic and somatic embryogenesis of carrot (Daucus carota L.). PhD Thesis, Wagennigen University, The Netherlands.
• Yakovlev MC, Tshaban JA. 1979. Ultrastructural aspects in embryogenesis of Reseda lutea. In: Yakovlev MC [ed.], Aktualnye voprosy embriologii pokrytosemennykh, 96-104. Nauka, Leningrad.
• Yeung EC, Zee SY and Ye XL. 1996. Embryology of Cymbidium sinense: embryo development. Annals of Botany 78:105-110.