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2006 | 15 | 1 |

Tytuł artykułu

Spontanoeously hypertensive rats are resistant to hypercholesterolaemia-idnuced atherosclerosis

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The objective of the present study was to re-evaluate critically the potential of spontaneously hypertensive rats (SHR) as models for diet-induced hypercholesterolaemia and the hypercholesterolaemia-induced atherosclerosis. Normotensive Wistar-Kyoto rats (WKY) were used as control animals. Twelve-months-old SHR (n=22) and WKY (n=17) male rats, were randomly divided into two groups each and fed one of the following diets for 12 consecutive weeks: the control diet i.e. the basal AIN-93M diet and the hypercholesterolaemic diet (g/100g: cholesterol 1, cholic acid 0.5 and butter 20). The hypercholesterolaemic diet increased highly significantly (P<0.001) serum total cholesterol concentrations in both WKY (control vs high-cholesterol) and SHR (control vs high cholesterol) rats (2.59±0.20 vs 6.54±0.33 and 2.52±0.20 vs 6.64±0.89 mmol/L, respectively). The same was true for LDL-cholesterol (0.75±0.09 vs 4.22±0.26 and 0.84±0.05 vs 4.62±0.69 mmol/L, respectively). HDL-cholesterol concentrations were also moderately increased in cholesterol-fed rats (1.46±0.10 vs 1.95±0.09 and 1.24±0.08 vs 1.53±0.17 mmol/L, respectively) whereas triglicerydes were unaffected. As regards lipid profile, the only effect of animal strain was that noted for SHR rats in which HDL cholesterol concentrations were significantly (P<0.05) lower than in WKY rats (1.39±0.10 vs 1.69±0.09 mmol/L, respectively). Serum concentrations of MCP-1 were elevated in both WKY (control vs high-cholesterol) and SHR rats (control vs high cholesterol, 41.22±7.98 vs 156.10±20.66 and 84.88±49.40 vs 181.65±38.40 pg/mL, respectively) though only in WKY rats this increase reached statistical significance. Either in WKY or SHR rats, the hypercholesterolaemic diet had no significant effect on endothelium-dependent nor endotheliumindependent vasodilation in aorta induced by acetylcholine or SNAP, respectively. Histological examination of proximal aortas from WKY (control vs high-cholesterol) and SHR (control vs highcholesterol) rats did not show any structural changes, indicative of atherosclerotic plaque formation. We conclude that diet induced hypercholesterolaemia does not lead to progression of atherosclerosis in SHR rats. Hence, hypertensive rats, fed hypercholesterolaemic diet, are not appropriate models for human hypercholesterolaemia and atherosclerosis.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

15

Numer

1

Opis fizyczny

p.103-114,fig.,ref.

Twórcy

  • Agricultural University of Krakow, Balicka 122, 30-149 Krakow, Poland
autor
autor
autor
autor

Bibliografia

  • Cappelli-Bigazzi M., Rubattu S., Battaglia C., Russo R., Enea I., Ambrosio G., Chiariello M., Volpe M., 1997. Effects of high-cholesterol and atherogenic diets on vascular relaxation in spontaneously hypertensive rats. Amer. J. Physiol. 273, H647-H654
  • Cheng J.B., Shibata S., 1981. Vascular relaxation in the spontaneously hypertensive rat. J. Cardiovasc. Pharmacol. 3, 1126-1140
  • Chłopicki S., Gryglewski R.J., 2004. Endothelial dysfunction in diabetes - a risk factor of atherothrombosis (in Polish). In: M. Tendera, K. Kawecka-Jaszcz, D. Czarnecka (Editors). Diabetes and Heart. Via Media, Gdańsk, pp. 1-19
  • Friedewald W.T., Levy R.I., Fredrickson D.S., 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative centrifuge. Clin. Chem. 18, 499-502
  • Guzik T.J., Adamek-Guzik T., Jopek A., Korbut R., 2004. Pathological mechanisms of vascular endothelial dysfunction in atherosclerosis (in Polish). Czynniki Ryzyka 1-2, 8-22
  • Hansson G.K., 2005. Inflammation, atherosclerosis and coronary artery disease. N. Engl. J. Med. 352, 1685-1695
  • Herrera M.D., Perez-Guerrero C., Marhuenda E., Ruiz-Gutierrez V., 2001. Effects of dietary oleicrich oils (virgin olive and high-oleic-acid sunflower) on vascular reactivity in Wistar-Kyoto and spontaneously hypertensive rats. Brit. J. Nutr. 86, 349-357
  • Hilpert K.F., Kris-Etherton P.M., West S.G., 2005. Lipid response to a low-fat diet with or without soy is modified by C-reactive protein status in moderately hypercholesterolemic adults. J. Nutr. 135, 1075-1079
  • Jawień J., Nastalek P., Korbut R., 2004. Mouse models of experimental atherosclerosis. J. Physiol. Pharmacol. 55, 503-517
  • Kataoka C., Egashira K., Inoue S., Takemoto M., Ni W., Koyanagi M., Kitamoto S., Usui M., Kaibuchi K., Shimokawa H., Takeshita A., 2002. Important role of Rho-kinase in the pathogenesis of cardiovascular inflammation and remodeling induced by long-term blockade of nitric oxide synthesis in rats. Hypertension 39, 245-250
  • Kitamoto S., Egashira K., 2004. Endothelial dysfunction and coronary atherosclerosis. Curr. Drug Targets Cardiovasc. Haematol. Disord. 4, 13-22
  • Moghadasian M.H., 2002. Experimental atherosclerosis: a historical overview. Life Sci. 70, 855- 865
  • Muller H., Lindman A.S., Brantsaeter A.L., Pedersen J.I., 2003. The serum LDL/HDL cholesterol ratio is influenced more favorably by exchanging saturated with unsaturated fat than by reducing saturated fat in the diet of women. J. Nutr. 133, 78-83
  • Pisulewski P.M., Kostogrys R.B., Lorkowska B., Bartuś M., Chłopicki S., 2005. Critical evaluation of normotensive rats as models for hypercholesterolaemia-induced atherosclerosis. J. Anim. Feed Sci. 14, 333-345
  • Reeves P.G., Nielsen F.H., Fahey G., 1993. AIN-93 purified diets for laboratory rodents: final report of the Amrican Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76 rodent diet. J. Nutr. 123, 1939-1951
  • Ross R., 1999. Atherosclerosis-an inflammatory disease. N. Engl. J. Med. 340, 115-126
  • Shirasaki Y., Kolm P., Nickols G.A., Lee T.J., 1988. Endothelial regulation of cyclic GMP and vascular responses in hypertension. J. Pharmacol. Exp. Ther. 245, 53-58
  • Sunano S., Osugi S., Shimamura K., 1989. Blood pressure and impairment of endothelium-dependent relaxation in spontaneously hypertensive rats. Experientia 45, 705-708
  • Vasconen T., Mervaala E., Krogerus L., Karppanen H., 2002. Suplementation of plant sterols and minerals benefits obese Zucker rats fed an atherogenic diet. J. Nutr. 132, 231-237
  • Yuan Y.V., Kitts D.D., Godin D.V., 1998. Variations in dietary fat and cholesterol intakes modify antioxidant status of SHR and WKY rats. J. Nutr. 128, 1620-1630
  • Zhao G., Etherton T.D., Martin K.R., West S.G., Gillies P.J., Kris-Etherton P.M., 2004. Dietary alphalinolenic acid reduces inflammatory and lipid cardiovascular risk factors in hypercholesterolemic men and women. J. Nutr. 134, 2991-2997
  • Zhang R., Ma J., Xia M., Zhu H., Ling W.H., 2004. Mild hypercholesterolemia induced by feeding rats diets rich in methionine or deficient in folate promotes early atherosclerotic inflammatory process. J. Nutr. 134, 825-830

Typ dokumentu

Bibliografia

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