Wplyw skrobi opornej RS4 na zawartosc wapnia w osoczu krwi i kosci udowej oraz na jego absorpcje i retencje pozorna u szczurow doswiadczalnych

• Autorzy: Grajeta H. , Prescha A. , Biernat J.

Warianty tytułu

EN

Effect of resistant starch RS4 on calcium content in blood plasma and femoral bone, and on its apparent absorption and retention in experimental rats

• Języki publikacji: PL

Abstrakty

PL

Badano wpływ skrobi opornej RS4 na zawartość wapnia w osoczu krwi i kości udowej oraz na jego absorpcję i retencję pozorną u szczurów doświadczalnych. Stwierdzono, że zastosowana w dietach skrobia RS4 nie miała wpływu na stężenie wapnia w osoczu krwi szczurów, natomiast zawartość tego pierwiastka w kości udowej oraz jego absorpcja i retencja pozorna były statystycznie istotnie wyższe u szczurów karmionych dietami z dodatkiem tej skrobi niż u szczurów karmionych dietami bez skrobi.

EN

The aim of this study was to investigate the effect of resistant starch RS4 (chemically-modified potato starch) on calcium content in blood plasma and the femoral bone, and on its apparent absorption and retention in experimental rats. The study was carried out on Wistar rats, which were divided into 6 groups (n=10) and fed the following diets: Group 1 – control diet without resistant starch and with 0,8% calcium ((K + 0,8% Ca); Group 2 – diet with 3% resistant starch and 0,8% calcium (RS4 + 0,8% Ca); Group 3 – control diet without resistant starch and with 1,3% calcium (K+ 1,3% Ca); and Group 4 – diet with 3% resistant starch and 1,3% calcium (RS4 + 1,3% Ca). One week before the end of the experiment, 3 rats from each group were housed individually in metabolic cages. After 3 days of adaptation, the rats’ urine and feces were collected during the last three days. At the end of the experiment, the rats were anesthetized with bioketane and then blood was taken by heart puncture and the femoral bone was prepared. Hemoglobin content was determined in the blood by the cyanmethemoglobin method and also the hematocrite value after centrifugation. Calcium content was measured in the blood serum using Biosystems test kit Arsenazo III. Calcium contents in the diets, feces, urine and femoral bone were determined by atomic emission spectroscopy method. The average calcium content in the blood serum of the rats fed diets RS4 + 0,8% Ca and RS4 + 1,3% Ca did not differ significantly from the serum calcium content of the animals fed control diets K+ 0,8% Ca and K+ 1,3% Ca. The average calcium contents in the femoral bone of the rats fed diets RS4 + 0,8% Ca and RS4 + 1,3% Ca amounted to 203,3 mg/g and 210,0 mg/g, respectively, and were significantly higher than the rats fed control diets K+ 0,8% Ca and K+ 1,3% Ca (153,8 mg/g and 179,0 mg/g, respectively). The apparent calcium absorption in the rats fed diets K+ 0,8% Ca and RS4 + 0,8% Ca was similar, but in the animals fed diet RS4 + 1,3% Ca was significantly higher (128,2 mg/day) than in the rats fed control diet K+ 1,3% Ca (94,1 mg/day). The apparent calcium retention in the rats fed diet RS4 + 1,3,% Ca was also significantly higher (124,4 mg/day) than the rats fed control diet K+ 1,3% Ca (91,3 mg/day). In conclusion, it was observed that the diets with RS4 have a beneficial effect on calcium content in the femoral bone, as well as calcium apparent absorption and retention in the test rats, but only in the case of a diet with higher amounts of this element (1,3%).

Słowa kluczowe

PL

skrobia oporna; procesy fizjologiczne; osocze; kosc udowa; wapn; zawartosc wapnia; absorpcja wapnia; retencja pozorna; zwierzeta laboratoryjne; szczury; absorpcja pozorna; dieta

EN

resistant starch; physiological process; plasma; femur; calcium; calcium content; calcium absorption; apparent retention; laboratory animal; rat; apparent absorption; diet

• Wydawca: -
• Czasopismo: Bromatologia i Chemia Toksykologiczna
• Rocznik: 2007
• Tom: 40
• Numer: 1
• Opis fizyczny: s.99-105,tab.,bibliogr.

Twórcy

autor

Grajeta H.

• Akademia Medyczna we Wroclawiu, pl.Nankiera 1, 50-140 Wroclaw

autor

Prescha A.

autor

Biernat J.